{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T01:09:38Z","timestamp":1772068178100,"version":"3.50.1"},"reference-count":20,"publisher":"Portland Press Ltd.","issue":"1","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":1270,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,2,1]]},"abstract":"<jats:p>Transmembrane protein 14A (TMEM14A) is a member of TMEMs. Alterations in TMEMs expression have been identified in several types of cancer, but the expression and function of TMEM14A in ovarian cancer is still unclear. Here, analysis on the expression data of the Cancer Genome Atlas (TCGA) ovarian serous cystadenocarcinoma (OV)\u00a0dataset demonstrated the overexpression of TMEM14A in ovarian cancer tissues compared with normal tissues, which was consistent with our real-time PCR analysis on ovarian cancer and normal tissues collected from 30 patients. In addition, TMEM14A knockdown in two ovarian cancer cell lines, A2780 and HO-8910, reduced cell proliferation, causes cell cycle arrest and suppressed cell invasion. Moreover, silencing of TMEM14A notably repressed G1\/S cell cycle transition and cell invasion via down-regulating the expression of cell cycle related proteins (Cyclin D1, Cyclin E and PCNA) and metastasis-related proteins (MMP-2 and MMP-9), respectively. TMEM14A knockdown significantly reduced the phosphorylation status of Smad2 and Smad3, downstream effectors of TGF-\u03b2 signalling. In summary, these results indicate that TMEM14A has a pro-tumorigenic effect in ovarian cancer cells, suggesting an important role of this protein in ovarian cancer oncogenesis and metastasis.<\/jats:p>","DOI":"10.1042\/bsr20150258","type":"journal-article","created":{"date-parts":[[2016,2,20]],"date-time":"2016-02-20T02:13:26Z","timestamp":1455934406000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells"],"prefix":"10.1042","volume":"36","author":[{"given":"Qingmei","family":"Zhang","sequence":"first","affiliation":[{"name":"FuJian University of Traditional Chinese Medicine, Fuzhou 350122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiufeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Longyan Hospital of Traditional Chinese Medicine, Longyan 364000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gynecology, the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, No. 602 of\u00a0817\u00a0Middle\u00a0Road,\u00a0Fuzhou 350004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingfen","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department of Gynecology, the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, No. 602 of\u00a0817\u00a0Middle\u00a0Road,\u00a0Fuzhou 350004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gynecology, the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, No. 602 of\u00a0817\u00a0Middle\u00a0Road,\u00a0Fuzhou 350004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2016,2,19]]},"reference":[{"key":"2021111618311562000_B1","doi-asserted-by":"publisher","first-page":"11","DOI":"10.3322\/caac.21166","article-title":"Cancer statistics, 2013","volume":"63","author":"Siegel","year":"2013","journal-title":"CA Cancer J. Clin."},{"key":"2021111618311562000_B2","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1038\/nrc2644","article-title":"The biology of ovarian cancer: new opportunities for translation","volume":"9","author":"Bast","year":"2009","journal-title":"Nat. Rev. Cancer"},{"key":"2021111618311562000_B3","doi-asserted-by":"publisher","first-page":"S161","DOI":"10.1016\/S0020-7292(06)60033-7","article-title":"Carcinoma of the ovary. FIGO 26th Annual Report on the Results of Treatment in Gynecological Cancer","volume":"95","author":"Heintz","year":"2006","journal-title":"Int. J. Gynaecol. Obstet."},{"key":"2021111618311562000_B4","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/j.canlet.2011.05.031","article-title":"TMEM14A inhibits N-(4-hydroxyphenyl) retinamide-induced apoptosis through the stabilization of mitochondrial membrane potential","volume":"309","author":"Jin","year":"2011","journal-title":"Cancer Lett."},{"key":"2021111618311562000_B5","first-page":"6","article-title":"The transmembrane protein TMEM7 is an IFN-\u03b1 responsive gene that suppresses cell proliferation and is down-regulated in human hepatocellular carcinoma","volume":"67","author":"Zhou","year":"2007","journal-title":"Cancer Res."},{"key":"2021111618311562000_B6","doi-asserted-by":"publisher","first-page":"11585","DOI":"10.18632\/oncotarget.3412","article-title":"TMEM16A overexpression contributes to tumor invasion and poor prognosis of human gastric cancer through TGF-beta signaling","volume":"6","author":"Liu","year":"2015","journal-title":"Oncotarget."},{"key":"2021111618311562000_B7","first-page":"305","article-title":"Involvement of TMEM22 overexpression in the growth of renal cell carcinoma cells","volume":"21","author":"Dobashi","year":"2009","journal-title":"Oncol. Rep."},{"key":"2021111618311562000_B8","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1016\/j.acthis.2011.12.006","article-title":"Abnormal accumulation of human transmembrane (TMEM)-176A and 176B proteins is associated with cancer pathology","volume":"114","author":"Cuajungco","year":"2012","journal-title":"Acta Histochem."},{"key":"2021111618311562000_B9","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1007\/s12032-012-0324-7","article-title":"Elevated expression of MAC30 predicts lymph node metastasis and unfavorable prognosis in patients with epithelial ovarian cancer","volume":"30","author":"Yang","year":"2013","journal-title":"Med. Oncol."},{"key":"2021111618311562000_B10","doi-asserted-by":"crossref","first-page":"3124","DOI":"10.3892\/or.2015.3902","article-title":"Inhibition of TMEM45A suppresses proliferation, induces cell cycle arrest and reduces cell invasion in human ovarian cancer cells","volume":"33","author":"Guo","year":"2015","journal-title":"Oncol. Rep."},{"key":"2021111618311562000_B11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13046-014-0119-0","article-title":"Overexpression of TMEM158 contributes to ovarian carcinogenesis","volume":"34","author":"Cheng","year":"2015","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"2021111618311562000_B12","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.ygeno.2010.01.004","article-title":"Comprehensive gene expression analysis of 5\u2032-end of mRNA identified novel intronic transcripts associated with hepatocellular carcinoma","volume":"95","author":"Hodo","year":"2010","journal-title":"Genomics"},{"key":"2021111618311562000_B13","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.3892\/ijo.2013.1878","article-title":"Hypoxia inducible factor 1\u03b1-mediated LOX expression correlates with migration and invasion in epithelial ovarian cancer","volume":"42","author":"Ji","year":"2013","journal-title":"Int. J. Oncol."},{"key":"2021111618311562000_B14","doi-asserted-by":"publisher","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021111618311562000_B15","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1136\/gut.35.4.530","article-title":"Proliferating cell nuclear antigen (PCNA): a new marker to study human colonic cell proliferation","volume":"35","author":"Kubben","year":"1994","journal-title":"Gut"},{"key":"2021111618311562000_B16","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1146\/annurev.pharmtox.39.1.295","article-title":"Cyclins and cell cycle checkpoints","volume":"39","author":"Johnson","year":"1999","journal-title":"Ann. Rev. Pharmacol. Toxicol."},{"key":"2021111618311562000_B17","doi-asserted-by":"publisher","first-page":"11585","DOI":"10.18632\/oncotarget.3412","article-title":"TMEM16A overexpression contributes to tumor invasion and poor prognosis of human gastric cancer through TGF-\u03b2 signaling","volume":"6","author":"Liu","year":"2015","journal-title":"Oncotarget."},{"key":"2021111618311562000_B18","doi-asserted-by":"publisher","first-page":"958","DOI":"10.1053\/j.gastro.2009.11.005","article-title":"Experimentally derived metastasis gene expression profile predicts recurrence and death in patients with colon cancer","volume":"138","author":"Smith","year":"2010","journal-title":"Gastroenterology"},{"key":"2021111618311562000_B19","doi-asserted-by":"publisher","first-page":"799","DOI":"10.1023\/A:1006723011835","article-title":"High levels of MMP-2, MMP-9, MT1-MMP and TIMP-2 mRNA correlate with poor survival in ovarian carcinoma","volume":"17","author":"Davidson","year":"1999","journal-title":"Clin. Exp. Metastasis"},{"key":"2021111618311562000_B20","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/j.bbadis.2008.01.006","article-title":"Role of transforming growth factor-beta superfamily signaling pathways in human disease","volume":"1782","author":"Gordon","year":"2008","journal-title":"Biochim. Biophys. Acta"}],"updated-by":[{"DOI":"10.1042\/bsr-2015-0258_eoc","type":"expression_of_concern","label":"Expression of concern","source":"retraction-watch","updated":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T00:00:00Z","timestamp":1675900800000},"record-id":"44020"},{"DOI":"10.1042\/bsr-2015-0258_ret","type":"retraction","label":"Retraction","source":"retraction-watch","updated":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T00:00:00Z","timestamp":1682035200000},"record-id":"44019"},{"DOI":"10.1042\/bsr-2015-0258_ret","type":"retraction","label":"Retraction","source":"publisher","updated":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T00:00:00Z","timestamp":1682035200000}},{"DOI":"10.1042\/bsr-2015-0258_eoc","type":"expression_of_concern","label":"Expression of concern","source":"publisher","updated":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T00:00:00Z","timestamp":1675900800000}}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20150258\/477541\/bsr036e298.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20150258\/477541\/bsr036e298.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T18:58:16Z","timestamp":1637089096000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/36\/1\/e00298\/56453\/Knockdown-of-TMEM14A-expression-by-RNAi-inhibits"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2,1]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,2,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20150258","relation":{"expression_of_concern":[{"id-type":"doi","id":"10.1042\/BSR-2015-0258_EOC","asserted-by":"object"}],"retraction":[{"id-type":"doi","id":"10.1042\/BSR-2015-0258_RET","asserted-by":"object"}]},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,1]]},"article-number":"e00298"}}