{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T15:28:07Z","timestamp":1772810887797,"version":"3.50.1"},"reference-count":37,"publisher":"Portland Press Ltd.","issue":"2","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":850,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,4,30]]},"abstract":"<jats:p>The present study aims to explore the correlation of human leucocyte antigen (HLA)-DQ and tumour necrosis factor (TNF)-\u03b1 gene polymorphisms with ocular myasthenia gravis (OMG) combined with thyroid-associated ophthalmopathy (TAO). From March 2009 to March 2015, 56 OMG patients complicated with TAO (OMG + TAO group), 134 patients diagnosed with OMG only (OMG group) and 236 healthy individuals (control group) were enrolled in the present study. PCR-sequence specific primer (PCR-SSP) was used for HLA-DQ genotyping and PCR-restriction fragment length polymorphism (PCR-RFLP) for TNF-\u03b1 genotyping. ELISA kit was applied to detect acetylcholine receptor antibody (AchRAb) level and chemiluminescence immunoassay (CLIA) to measure thyroid-associated antibody (T-Ab) level. Logistic regression analysis was carried out to analyse the risk factors for OMG combined with TAO. DQA1*0103 showed lower frequency in the OMG group than in the control group. DQA1*0301 showed increased and DQB1*0601 showed decreased frequency in the OMG + TAO group. DQB1*0501 showed higher frequency in the OMG and OMG + TAO groups than in the control group. Patients carrying TNF-\u03b1 -863C &amp;gt; A (CA + AA) might confront with greater risks of OMG combined with TAO. Frequency of DQA1*0103\/*0301 and DQB1*0501\/*0601, and TNF-\u03b1 -863C &amp;gt; A, -238G &amp;gt; A and -308G &amp;gt; A were associated with the levels of AchRAb and T-Ab. TNF-\u03b1 -863C &amp;gt; A (CA + AA) and high level of T-Ab were risk factors for OMG combined with TAO. Our results demonstrate that TNF-\u03b1 -863 polymorphism is possibly correlated with the risk of OMG combined with TAO.<\/jats:p>","DOI":"10.1042\/bsr20160440","type":"journal-article","created":{"date-parts":[[2017,1,24]],"date-time":"2017-01-24T21:00:25Z","timestamp":1485291625000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Correlation of\n                    <i>HLA-DQ<\/i>\n                    and\n                    <i>TNF-\u03b1<\/i>\n                    gene polymorphisms with ocular myasthenia gravis combined with thyroid-associated ophthalmopathy"],"prefix":"10.1042","volume":"37","author":[{"given":"Hong-Wei","family":"Yang","sequence":"first","affiliation":[{"name":"Geriatric Ward of Neurology, Tianjin Geriatrics Institute, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-Xue","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, The First Affiliated Hospital of Jinan University, Shipai, Guangzhou 510630, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Bao","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu-Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Lei","sequence":"additional","affiliation":[{"name":"Geriatric Ward of Neurology, Tianjin Geriatrics Institute, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao-Lin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Neurology, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2017,3,27]]},"reference":[{"key":"2021111619075746900_B1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s00005-011-0158-6","article-title":"Acetylcholine receptor-induced experimental myasthenia gravis: what have we learned from animal models after three decades?","volume":"60","author":"Baggi","year":"2012","journal-title":"Arch. Immunol. Ther. Exp. 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Neurol."},{"key":"2021111619075746900_B4","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.ophtha.2009.05.029","article-title":"Rituximab treatment of patients with severe, corticosteroid-resistant thyroid-associated ophthalmopathy","volume":"117","author":"Khanna","year":"2010","journal-title":"Ophthalmology"},{"key":"2021111619075746900_B5","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1177\/0883073808324777","article-title":"Ocular myasthenia gravis and Graves disease in a 10-year-old child","volume":"24","author":"Koves","year":"2009","journal-title":"J. Child Neurol."},{"key":"2021111619075746900_B6","first-page":"100","article-title":"Ocular myasthenia gravis coincident with thyroid ophthalmopathy","volume":"51","author":"Yaman","year":"2003","journal-title":"Neurol. 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Neuroimmunol."},{"key":"2021111619075746900_B10","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/CVI.00247-06","article-title":"Relationship between HLA polymorphisms and gamma interferon and interleukin-10 cytokine production in healthy individuals after rubella vaccination","volume":"14","author":"Ovsyannikova","year":"2007","journal-title":"Clin. Vaccine Immunol."},{"key":"2021111619075746900_B11","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/1745-6150-3-42","article-title":"A new model defines the minimal set of polymorphism in HLA-DQ and -DR that determines susceptibility and resistance to autoimmune diabetes","volume":"3","author":"Parry","year":"2008","journal-title":"Biol. Direct"},{"key":"2021111619075746900_B12","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.humimm.2006.02.039","article-title":"HLA-DQ polymorphism in Turkish patients with myasthenia gravis","volume":"67","author":"Saruhan-Direskeneli","year":"2006","journal-title":"Hum. Immunol."},{"key":"2021111619075746900_B13","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.clineuro.2012.04.001","article-title":"Association of HLA-DR\/DQ polymorphism with myasthenia gravis in Tunisian patients","volume":"115","author":"Fekih-Mrissa","year":"2013","journal-title":"Clin. Neurol. 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Clin. Endocrinol. Metab."},{"key":"2021111619075746900_B19","first-page":"43","article-title":"Reverse \u2018see-saw\u2019 relationship between Graves\u2019 disease and myasthenia gravis; clinical and immunological studies","volume":"52","author":"Sekiguchi","year":"2005","journal-title":"J. Med. Dent. Sci."},{"key":"2021111619075746900_B20","doi-asserted-by":"crossref","first-page":"237","DOI":"10.3109\/08916939309077371","article-title":"Lack of disease associated HLA-DQ restriction fragment length polymorphisms in families with autoimmune thyroid disease","volume":"14","author":"O\u2019Connor","year":"1993","journal-title":"Autoimmunity"},{"key":"2021111619075746900_B21","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1002\/mus.21748","article-title":"Comment on myasthenia gravis associated with tnf-alpha receptor blockers: a multifaceted issue","volume":"42","author":"Galassi","year":"2010","journal-title":"Muscle Nerve"},{"key":"2021111619075746900_B22","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1001\/jamaneurol.2015.0203","article-title":"Clinical features and diagnostic usefulness of antibodies to clustered acetylcholine receptors in the diagnosis of seronegative myasthenia gravis","volume":"72","author":"Rodriguez Cruz","year":"2015","journal-title":"JAMA Neurol."},{"key":"2021111619075746900_B23","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/S0002-9394(14)72787-4","article-title":"Diagnostic criteria for Graves\u2019 ophthalmopathy","volume":"119","author":"Bartley","year":"1995","journal-title":"Am. 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