{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T03:58:13Z","timestamp":1767153493982,"version":"build-2238731810"},"reference-count":20,"publisher":"Portland Press Ltd.","issue":"2","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":875,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,4,30]]},"abstract":"<jats:p>Oral tongue squamous cell carcinoma (OTSCC) is the most common type of oral carcinomas. However, the molecular mechanism by which OTSCC developed is not fully identified. Stromal interaction molecule 1 (STIM1) is a transmembrane protein, mainly located in the endoplasmic reticulum (ER). STIM1 is involved in several types of cancers. Here, we report that STIM1 contributes to the development of human OTSCC. We knocked down STIM1 in OTSCC cell line Tca-8113 with lentivirus-mediated shRNA and found that STIM1 knockdown repressed the proliferation of Tca-8113 cells. In addition, we also showed that STIM1 deficiency reduced colony number of Tca-8113 cells. Knockdown of STIM1 repressed cells to enter M phase of cell cycle and induced cellular apoptosis. Furthermore, we performed microarray and bioinformatics analysis and found that STIM1 was associated with p53 and MAPK pathways, which may contribute to the effects of STIM1 on cell growth, cell cycle, and apoptosis. Finally, we confirmed that STIM1 controlled the expression of MDM2, cyclin-dependent kinase 4 (CDK4), and growth arrest and DNA damage inducible \u03b1 (GADD45A) in OTSCC cells. In conclusion, we provide evidence that STIM1 contributes to the development of OTSCC partially through regulating p53 and MAPK pathways to promote cell cycle and survival.<\/jats:p>","DOI":"10.1042\/bsr20160519","type":"journal-article","created":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T20:50:34Z","timestamp":1484945434000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Stromal interaction molecule 1 regulates growth, cell cycle, and apoptosis of human tongue squamous carcinoma cells"],"prefix":"10.1042","volume":"37","author":[{"given":"Xiaobo","family":"Cui","sequence":"first","affiliation":[{"name":"Section of Inner Mongolia Medical University Affiliated Hospital Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laixiao","family":"Song","sequence":"additional","affiliation":[{"name":"Section of Inner Mongolia Medical University Affiliated Hospital Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfei","family":"Bai","sequence":"additional","affiliation":[{"name":"Section of Inner Mongolia Medical University Affiliated Hospital Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaping","family":"Wang","sequence":"additional","affiliation":[{"name":"Section of Inner Mongolia Medical University Affiliated Hospital Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boqian","family":"Wang","sequence":"additional","affiliation":[{"name":"Section of Inner Mongolia Medical University Affiliated Hospital Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Section of Inner Mongolia Medical University Affiliated Hospital Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2017,3,2]]},"reference":[{"key":"2021111619082907700_B1","doi-asserted-by":"crossref","first-page":"5567","DOI":"10.7314\/APJCP.2013.14.10.5567","article-title":"Epidemiology of oral cancer in Asia in the past decade\u2013an update (2000-2012)","volume":"14","author":"Krishna Rao","year":"2013","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"2021111619082907700_B2","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/jcmm.12650","article-title":"MicroRNA expression and its implications for diagnosis and therapy of tongue squamous cell carcinoma","volume":"20","author":"Yu","year":"2016","journal-title":"J. Cell. Mol. Med."},{"key":"2021111619082907700_B3","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1016\/j.bbamcr.2015.10.003","article-title":"STIM and calcium channel complexes in cancer","volume":"1863","author":"Jardin","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"2021111619082907700_B4","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1038\/nature04147","article-title":"STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane","volume":"437","author":"Zhang","year":"2005","journal-title":"Nature"},{"key":"2021111619082907700_B5","doi-asserted-by":"crossref","first-page":"4040","DOI":"10.1073\/pnas.0510050103","article-title":"STIM1 has a plasma membrane role in the activation of store-operated Ca2+ channels","volume":"103","author":"Spassova","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021111619082907700_B6","doi-asserted-by":"crossref","first-page":"11754","DOI":"10.1038\/srep11754","article-title":"STIM1 accelerates cell senescence in a remodeled microenvironment but enhances the epithelial-to-mesenchymal transition in prostate cancer","volume":"5","author":"Xu","year":"2015","journal-title":"Sci. Rep."},{"key":"2021111619082907700_B7","doi-asserted-by":"crossref","first-page":"4808","DOI":"10.1038\/onc.2014.404","article-title":"STIM1, a direct target of microRNA-185, promotes tumor metastasis and is associated with poor prognosis in colorectal cancer","volume":"34","author":"Zhang","year":"2016","journal-title":"Oncogene"},{"key":"2021111619082907700_B8","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1007\/s12032-015-0608-9","article-title":"Stromal interaction molecule 1 (STIM1) silencing inhibits tumor growth and promotes cell cycle arrest and apoptosis in hypopharyngeal carcinoma","volume":"32","author":"Sun","year":"2015","journal-title":"Med. Oncol."},{"key":"2021111619082907700_B9","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.cell.2011.02.013","article-title":"Hallmarks of cancer: the next generation","volume":"144","author":"Hanahan","year":"2011","journal-title":"Cell"},{"key":"2021111619082907700_B10","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/j.tcb.2013.07.006","article-title":"Evading apoptosis in cancer","volume":"23","author":"Fernald","year":"2013","journal-title":"Trends Cell Biol."},{"key":"2021111619082907700_B11","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.celrep.2015.06.033","article-title":"STIM1 mediates hypoxia-driven hepatocarcinogenesis via interaction with HIF-1","volume":"12","author":"Li","year":"2015","journal-title":"Cell Rep."},{"key":"2021111619082907700_B12","doi-asserted-by":"crossref","first-page":"4358","DOI":"10.1038\/onc.2014.366","article-title":"STIM1 overexpression promotes colorectal cancer progression, cell motility and COX-2 expression","volume":"34","author":"Wang","year":"2015","journal-title":"Oncogene"},{"key":"2021111619082907700_B13","doi-asserted-by":"crossref","first-page":"2263","DOI":"10.1016\/j.bbamcr.2014.02.012","article-title":"Orai1 and STIM1 mediate SOCE and contribute to apoptotic resistance of pancreatic adenocarcinoma","volume":"1843","author":"Kondratska","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"2021111619082907700_B14","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/j.etp.2013.04.003","article-title":"The apoptosis of non-small cell lung cancer induced by cisplatin through modulation of STIM1","volume":"65","author":"Li","year":"2013","journal-title":"Exp. Toxicol. Pathol."},{"key":"2021111619082907700_B15","doi-asserted-by":"crossref","first-page":"3496","DOI":"10.3892\/or.2016.4704","article-title":"Stromal interaction molecule 1 plays an important role in gastric cancer progression","volume":"35","author":"Xu","year":"2016","journal-title":"Oncol. Rep."},{"key":"2021111619082907700_B16","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nrclinonc.2010.41","article-title":"The molecular pathology of cancer","volume":"7","author":"Harris","year":"2010","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"2021111619082907700_B17","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1101\/gad.13.5.607","article-title":"Control of cell cycle progression by c-Jun is p53 dependent","volume":"13","author":"Schreiber","year":"1999","journal-title":"Genes Dev."},{"key":"2021111619082907700_B18","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.mrfmmm.2004.06.055","article-title":"Gadd45a, a p53- and BRCA1-regulated stress protein, in cellular response to DNA damage","volume":"569","author":"Zhan","year":"2005","journal-title":"Mutat. Res."},{"key":"2021111619082907700_B19","doi-asserted-by":"crossref","first-page":"7517","DOI":"10.1038\/sj.onc.1210557","article-title":"Base excision DNA repair defect in Gadd45a-deficient cells","volume":"26","author":"Jung","year":"2007","journal-title":"Oncogene"},{"key":"2021111619082907700_B20","doi-asserted-by":"crossref","first-page":"17030","DOI":"10.1074\/jbc.M112.411272","article-title":"Store-operated Ca2+ entry (SOCE) induced by protease-activated receptor-1 mediates STIM1 protein phosphorylation to inhibit SOCE in endothelial cells through AMP-activated protein kinase and p38beta mitogen-activated protein kinase","volume":"288","author":"Sundivakkam","year":"2013","journal-title":"J. Biol. Chem."}],"updated-by":[{"DOI":"10.1042\/bsr-2016-0519_ret","type":"retraction","label":"Retraction","source":"retraction-watch","updated":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T00:00:00Z","timestamp":1677715200000},"record-id":"43202"},{"DOI":"10.1042\/bsr-20160519_eoc","type":"expression_of_concern","label":"Expression of concern","source":"retraction-watch","updated":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T00:00:00Z","timestamp":1618790400000},"record-id":"43203"},{"DOI":"10.1042\/bsr-20160519_eoc","type":"expression_of_concern","label":"Expression of concern","source":"publisher","updated":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T00:00:00Z","timestamp":1618790400000}},{"DOI":"10.1042\/bsr-2016-0519_ret","type":"retraction","label":"Retraction","source":"publisher","updated":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T00:00:00Z","timestamp":1677715200000}}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20160519\/518587\/bsr-2016-0519.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20160519\/518587\/bsr-2016-0519.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T14:56:00Z","timestamp":1637074560000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/37\/2\/BSR20160519\/82795\/Stromal-interaction-molecule-1-regulates-growth"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,2]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,4,30]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20160519","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,2]]},"article-number":"BSR20160519"}}