{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T18:55:42Z","timestamp":1791658542473,"version":"4.3.4"},"reference-count":211,"publisher":"Portland Press Ltd.","issue":"6","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":597,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,12,22]]},"abstract":"<jats:p>Transposons derived from Sleeping Beauty (SB), piggyBac (PB), or Tol2 typically require cotransfection of transposon DNA with a transposase either as an expression plasmid or mRNA. Consequently, this results in genomic integration of the potentially therapeutic gene into chromosomes of the desired target cells, and thus conferring stable expression. Non-viral transfection methods are typically preferred to deliver the transposon components into the target cells. However, these methods do not match the efficacy typically attained with viral vectors and are sometimes associated with cellular toxicity evoked by the DNA itself. In recent years, the overall transposition efficacy has gradually increased by codon optimization of the transposase, generation of hyperactive transposases, and\/or introduction of specific mutations in the transposon terminal repeats. Their versatility enabled the stable genetic engineering in many different primary cell types, including stem\/progenitor cells and differentiated cell types. This prompted numerous preclinical proof-of-concept studies in disease models that demonstrated the potential of DNA transposons for ex vivo and in vivo gene therapy. One of the merits of transposon systems relates to their ability to deliver relatively large therapeutic transgenes that cannot readily be accommodated in viral vectors such as full-length dystrophin cDNA. These emerging insights paved the way toward the first transposon-based phase I\/II clinical trials to treat hematologic cancer and other diseases. Though encouraging results were obtained, controlled pivotal clinical trials are needed to corroborate the efficacy and safety of transposon-based therapies.<\/jats:p>","DOI":"10.1042\/bsr20160614","type":"journal-article","created":{"date-parts":[[2017,10,31]],"date-time":"2017-10-31T20:10:49Z","timestamp":1509480649000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["Preclinical and clinical advances in transposon-based gene therapy"],"prefix":"10.1042","volume":"37","author":[{"given":"Jaitip","family":"Tipanee","sequence":"first","affiliation":[{"name":"Department of Gene Therapy and Regenerative Medicine, Free University of Brussels (VUB), Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoke\u00a0Chin","family":"Chai","sequence":"additional","affiliation":[{"name":"Department of Gene Therapy and Regenerative Medicine, Free University of Brussels (VUB), Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"VandenDriessche","sequence":"additional","affiliation":[{"name":"Department of Gene Therapy and Regenerative Medicine, Free University of Brussels (VUB), Brussels, Belgium"},{"name":"Department of Cardiovascular Sciences, Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marinee\u00a0K.","family":"Chuah","sequence":"additional","affiliation":[{"name":"Department of Gene Therapy and Regenerative Medicine, Free University of Brussels (VUB), Brussels, Belgium"},{"name":"Department of Cardiovascular Sciences, Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2017,12,5]]},"reference":[{"key":"2021111618561100900_B1","doi-asserted-by":"crossref","first-page":"737","DOI":"10.15252\/emmm.201707573","article-title":"Gene therapy for ADA-SCID, the first marketing approval of an ex vivo gene therapy in Europe: paving the road for the next generation of advanced therapy medicinal products","volume":"9","author":"Aiuti","year":"2017","journal-title":"EMBO Mol. Med."},{"key":"2021111618561100900_B2","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1056\/NEJMoa0805817","article-title":"Gene therapy for immunodeficiency due to adenosine deaminase deficiency","volume":"360","author":"Aiuti","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"2021111618561100900_B3","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1038\/nm1358","article-title":"Successful transduction of liver in hemophilia by AAV-Factor IX and limitations imposed by the host immune response","volume":"12","author":"Manno","year":"2006","journal-title":"Nat. Med."},{"key":"2021111618561100900_B4","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1126\/science.1088547","article-title":"LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1","volume":"302","author":"Hacein-Bey-Abina","year":"2003","journal-title":"Science"},{"key":"2021111618561100900_B5","doi-asserted-by":"crossref","first-page":"3132","DOI":"10.1172\/JCI35700","article-title":"Insertional oncogenesis in 4 patients after retrovirus-mediated gene therapy of SCID-X1","volume":"118","author":"Hacein-Bey-Abina","year":"2008","journal-title":"J. Clin. Invest."},{"key":"2021111618561100900_B6","doi-asserted-by":"crossref","first-page":"227ra33","DOI":"10.1126\/scitranslmed.3007280","article-title":"Gene therapy for Wiskott-Aldrich syndrome\u2013long-term efficacy and genotoxicity","volume":"6","author":"Braun","year":"2014","journal-title":"Sci. Transl. Med."},{"key":"2021111618561100900_B7","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1038\/nature09328","article-title":"Transfusion independence and HMGA2 activation after gene therapy of human beta-thalassaemia","volume":"467","author":"Cavazzana-Calvo","year":"2010","journal-title":"Nature"},{"key":"2021111618561100900_B8","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1182\/blood-2015-09-618587","article-title":"Genetic treatment of a molecular disorder: gene therapy approaches to sickle cell disease","volume":"127","author":"Hoban","year":"2016","journal-title":"Blood"},{"key":"2021111618561100900_B9","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1517\/14712598.2012.684875","article-title":"Recent developments in transposon-mediated gene therapy","volume":"12","author":"Di Matteo","year":"2012","journal-title":"Expert Opin. Biol. Ther."},{"key":"2021111618561100900_B10","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1182\/blood-2009-04-210427","article-title":"Emerging potential of transposons for gene therapy and generation of induced pluripotent stem cells","volume":"114","author":"VandenDriessche","year":"2009","journal-title":"Blood"},{"key":"2021111618561100900_B11","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/1759-8753-1-25","article-title":"The expanding universe of transposon technologies for gene and cell engineering","volume":"1","author":"Ivics","year":"2010","journal-title":"Mob. DNA"},{"key":"2021111618561100900_B12","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1089\/hum.2011.143","article-title":"Nonviral gene delivery with the sleeping beauty transposon system","volume":"22","author":"Ivics","year":"2011","journal-title":"Hum. Gene Ther."},{"key":"2021111618561100900_B13","doi-asserted-by":"crossref","first-page":"341","DOI":"10.2174\/156652311797415827","article-title":"Efficacy and safety of Sleeping Beauty transposon-mediated gene transfer in preclinical animal studies","volume":"11","author":"Hackett","year":"2011","journal-title":"Curr. Gene Ther."},{"key":"2021111618561100900_B14","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1101\/gr.5826307","article-title":"The evolutionary history of human DNA transposons: evidence for intense activity in the primate lineage","volume":"17","author":"Pace","year":"2007","journal-title":"Genome Res."},{"key":"2021111618561100900_B15","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.ymthe.2005.10.014","article-title":"RNA as a source of transposase for Sleeping Beauty-mediated gene insertion and expression in somatic cells and tissues","volume":"13","author":"Wilber","year":"2006","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B16","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1038\/sj.mt.6300160","article-title":"Messenger RNA as a source of transposase for sleeping beauty transposon-mediated correction of hereditary tyrosinemia type I","volume":"15","author":"Wilber","year":"2007","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B17","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1038\/mt.2010.47","article-title":"Comparative analysis of transposable element vector systems in human cells","volume":"18","author":"Grabundzija","year":"2010","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B18","doi-asserted-by":"crossref","first-page":"15008","DOI":"10.1073\/pnas.0606979103","article-title":"piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells","volume":"103","author":"Wu","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021111618561100900_B19","doi-asserted-by":"crossref","first-page":"6205","DOI":"10.1016\/j.febslet.2005.10.004","article-title":"Functional zinc finger\/sleeping beauty transposase chimeras exhibit attenuated overproduction inhibition","volume":"579","author":"Wilson","year":"2005","journal-title":"FEBS Lett."},{"key":"2021111618561100900_B20","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.omtn.2017.02.002","article-title":"Engineering of PEDF-expressing primary pigment epithelial cells by the SB transposon system delivered by pFAR4 plasmids","volume":"6","author":"Thumann","year":"2017","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"2021111618561100900_B21","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1038\/leu.2016.180","article-title":"Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors","volume":"31","author":"Monjezi","year":"2017","journal-title":"Leukemia"},{"key":"2021111618561100900_B22","doi-asserted-by":"crossref","first-page":"e74","DOI":"10.1038\/mtna.2013.1","article-title":"Efficient sleeping beauty DNA transposition from DNA minicircles","volume":"2","author":"Sharma","year":"2013","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"2021111618561100900_B23","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1007\/BF00282750","article-title":"Widespread occurrence of the Tc1 transposon family: Tc1-like transposons from teleost fish","volume":"244","author":"Radice","year":"1994","journal-title":"Mol. Gen. Genet."},{"key":"2021111618561100900_B24","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/S0092-8674(00)80436-5","article-title":"Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells","volume":"91","author":"Ivics","year":"1997","journal-title":"Cell"},{"key":"2021111618561100900_B25","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/S1525-0016(03)00099-6","article-title":"Gene transfer into genomes of human cells by the sleeping beauty transposon system","volume":"8","author":"Geurts","year":"2003","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B26","doi-asserted-by":"crossref","first-page":"e50","DOI":"10.1093\/nar\/gkm089","article-title":"Site-directed transposon integration in human cells","volume":"35","author":"Yant","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"2021111618561100900_B27","doi-asserted-by":"crossref","first-page":"e76771","DOI":"10.1371\/journal.pone.0076771","article-title":"Hybrid adeno-associated viral vectors utilizing transposase-mediated somatic integration for stable transgene expression in human cells","volume":"8","author":"Zhang","year":"2013","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B28","doi-asserted-by":"crossref","first-page":"e337","DOI":"10.1038\/mtna.2016.44","article-title":"A high-capacity adenoviral hybrid vector system utilizing the hyperactive sleeping beauty transposase SB100X for enhanced integration","volume":"5","author":"Boehme","year":"2016","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"2021111618561100900_B29","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1038\/ng.343","article-title":"Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates","volume":"41","author":"Mates","year":"2009","journal-title":"Nat. Genet."},{"key":"2021111618561100900_B30","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1093\/nar\/gkv1464","article-title":"piggyBac transposons expressing full-length human dystrophin enable genetic correction of dystrophic mesoangioblasts","volume":"44","author":"Loperfido","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"2021111618561100900_B31","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.1002\/stem.501","article-title":"Novel hyperactive transposons for genetic modification of induced pluripotent and adult stem cells: a nonviral paradigm for coaxed differentiation","volume":"28","author":"Belay","year":"2010","journal-title":"Stem Cells"},{"key":"2021111618561100900_B32","doi-asserted-by":"crossref","first-page":"11126","DOI":"10.1038\/ncomms11126","article-title":"Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering","volume":"7","author":"Voigt","year":"2016","journal-title":"Nat. Commun."},{"key":"2021111618561100900_B33","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.ymthe.2003.11.024","article-title":"Development of hyperactive sleeping beauty transposon vectors by mutational analysis","volume":"9","author":"Zayed","year":"2004","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B34","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1038\/gt.2009.144","article-title":"Extending the transposable payload limit of Sleeping Beauty (SB) using the Herpes Simplex Virus (HSV)\/SB amplicon-vector platform","volume":"17","author":"de Silva","year":"2010","journal-title":"Gene Ther."},{"key":"2021111618561100900_B35","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1128\/jvi.18.3.1040-1050.1976","article-title":"Strain selection during serial passage of Trichoplusia in nuclear polyhedrosis virus","volume":"18","author":"Potter","year":"1976","journal-title":"J. Virol."},{"key":"2021111618561100900_B36","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/0042-6822(82)90476-7","article-title":"The isolation and characterization of the MP and FP plaque variants of Galleria mellonella nuclear polyhedrosis virus","volume":"117","author":"Fraser","year":"1982","journal-title":"Virology"},{"key":"2021111618561100900_B37","doi-asserted-by":"crossref","first-page":"MDNA3","DOI":"10.1128\/microbiolspec.MDNA3-0028-2014","article-title":"piggyBac transposon","volume":"3","author":"Yusa","year":"2015","journal-title":"Microbiol. Spectr."},{"key":"2021111618561100900_B38","doi-asserted-by":"crossref","first-page":"50","DOI":"10.3109\/10409230903505596","article-title":"Integrating prokaryotes and eukaryotes: DNA transposases in light of structure","volume":"45","author":"Hickman","year":"2010","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"2021111618561100900_B39","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1038\/emboj.2008.41","article-title":"piggyBac can bypass DNA synthesis during cut and paste transposition","volume":"27","author":"Mitra","year":"2008","journal-title":"EMBO J."},{"key":"2021111618561100900_B40","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.stem.2009.07.011","article-title":"An efficient and reversible transposable system for gene delivery and lineage-specific differentiation in human embryonic stem cells","volume":"5","author":"Lacoste","year":"2009","journal-title":"Cell Stem Cell"},{"key":"2021111618561100900_B41","doi-asserted-by":"crossref","first-page":"e87","DOI":"10.1093\/nar\/gkm446","article-title":"Generation of an inducible and optimized piggyBac transposon system","volume":"35","author":"Cadinanos","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"2021111618561100900_B42","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1073\/pnas.1008322108","article-title":"A hyperactive piggyBac transposase for mammalian applications","volume":"108","author":"Yusa","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021111618561100900_B43","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.1038\/mt.2014.131","article-title":"Hyperactive piggyBac transposons for sustained and robust liver-targeted gene therapy","volume":"22","author":"Di Matteo","year":"2014","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B44","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1089\/10430340150504046","article-title":"Efficient expression of naked DNA delivered intraarterially to limb muscles of nonhuman primates","volume":"12","author":"Zhang","year":"2001","journal-title":"Hum. Gene Ther."},{"key":"2021111618561100900_B45","doi-asserted-by":"crossref","first-page":"e150","DOI":"10.1093\/nar\/gks643","article-title":"Transposon-mediated BAC transgenesis in human ES cells","volume":"40","author":"Rostovskaya","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"2021111618561100900_B46","doi-asserted-by":"crossref","first-page":"e148","DOI":"10.1093\/nar\/gkr764","article-title":"Mobilization of giant piggyBac transposons in the mouse genome","volume":"39","author":"Li","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"2021111618561100900_B47","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1096\/fj.12-205526","article-title":"Transposon-mediated transgenesis, transgenic rescue, and tissue-specific gene expression in rodents and rabbits","volume":"27","author":"Katter","year":"2013","journal-title":"FASEB J."},{"key":"2021111618561100900_B48","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/383030a0","article-title":"Transposable element in fish","volume":"383","author":"Koga","year":"1996","journal-title":"Nature"},{"key":"2021111618561100900_B49","doi-asserted-by":"crossref","first-page":"e169","DOI":"10.1371\/journal.pgen.0020169","article-title":"Harnessing a high cargo-capacity transposon for genetic applications in vertebrates","volume":"2","author":"Balciunas","year":"2006","journal-title":"PLoS Genet."},{"key":"2021111618561100900_B50","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/gt.2014.104","article-title":"The Tol2 transposon system mediates the genetic engineering of T-cells with CD19-specific chimeric antigen receptors for B-cell malignancies","volume":"22","author":"Tsukahara","year":"2015","journal-title":"Gene Ther."},{"key":"2021111618561100900_B51","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s13100-016-0062-z","article-title":"Active recombinant Tol2 transposase for gene transfer and gene discovery applications","volume":"7","author":"Ni","year":"2016","journal-title":"Mob. DNA"},{"key":"2021111618561100900_B52","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1534\/genetics.109.100768","article-title":"Efficient transposition of Tol2 in the mouse germline","volume":"183","author":"Keng","year":"2009","journal-title":"Genetics"},{"key":"2021111618561100900_B53","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/1472-6750-11-28","article-title":"Genome-wide target profiling of piggyBac and Tol2 in HEK 293: pros and cons for gene discovery and gene therapy","volume":"11","author":"Meir","year":"2011","journal-title":"BMC Biotechnol."},{"key":"2021111618561100900_B54","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1534\/genetics.106.060244","article-title":"Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition","volume":"174","author":"Urasaki","year":"2006","journal-title":"Genetics"},{"key":"2021111618561100900_B55","first-page":"E47","article-title":"Stable somatic gene expression in mouse lungs following electroporation-mediated Tol2 transposon delivery","volume":"61","author":"Muliawan","year":"2015","journal-title":"Kobe J. Med. Sci."},{"key":"2021111618561100900_B56","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.jbiotec.2015.11.024","article-title":"Transgenesis of Tol2-mediated seamlessly constructed BAC mammary gland expression vectors in Mus musculus","volume":"218","author":"Yang","year":"2016","journal-title":"J. Biotechnol."},{"key":"2021111618561100900_B57","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.ygeno.2010.02.006","article-title":"A simple and highly efficient transgenesis method in mice with the Tol2 transposon system and cytoplasmic microinjection","volume":"95","author":"Sumiyama","year":"2010","journal-title":"Genomics"},{"key":"2021111618561100900_B58","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1186\/1471-2164-10-477","article-title":"Transposon-mediated BAC transgenesis in zebrafish and mice","volume":"10","author":"Suster","year":"2009","journal-title":"BMC Genomics"},{"key":"2021111618561100900_B59","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1002\/dvg.20234","article-title":"Tol2 transposon-mediated transgenesis in Xenopus tropicalis","volume":"44","author":"Hamlet","year":"2006","journal-title":"Genesis"},{"key":"2021111618561100900_B60","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/j.ydbio.2007.01.043","article-title":"Stable integration and conditional expression of electroporated transgenes in chicken embryos","volume":"305","author":"Sato","year":"2007","journal-title":"Dev. Biol."},{"key":"2021111618561100900_B61","doi-asserted-by":"crossref","first-page":"19827","DOI":"10.1073\/pnas.0810380105","article-title":"Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish","volume":"105","author":"Urasaki","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021111618561100900_B62","doi-asserted-by":"crossref","first-page":"1998","DOI":"10.1038\/nprot.2011.416","article-title":"Transposon-mediated BAC transgenesis in zebrafish","volume":"6","author":"Suster","year":"2011","journal-title":"Nat. Protoc."},{"key":"2021111618561100900_B63","doi-asserted-by":"crossref","first-page":"e112712","DOI":"10.1371\/journal.pone.0112712","article-title":"Genomic analysis of Sleeping Beauty transposon integration in human somatic cells","volume":"9","author":"Turchiano","year":"2014","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B64","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1007\/s10540-005-2793-9","article-title":"Counterselection and co-delivery of transposon and transposase functions for Sleeping Beauty-mediated transposition in cultured mammalian cells","volume":"24","author":"Converse","year":"2004","journal-title":"Biosci. Rep."},{"key":"2021111618561100900_B65","doi-asserted-by":"crossref","first-page":"5004","DOI":"10.1073\/pnas.1218620110","article-title":"Simple piggyBac transposon-based mammalian cell expression system for inducible protein production","volume":"110","author":"Li","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021111618561100900_B66","doi-asserted-by":"crossref","first-page":"e77222","DOI":"10.1371\/journal.pone.0077222","article-title":"A novel piggyBac transposon inducible expression system identifies a role for AKT signalling in primordial germ cell migration","volume":"8","author":"Glover","year":"2013","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B67","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/1472-6750-13-75","article-title":"Exogenous mRNA delivery and bioavailability in gene transfer mediated by piggyBac transposition","volume":"13","author":"Bire","year":"2013","journal-title":"BMC Biotechnol."},{"key":"2021111618561100900_B68","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.scr.2014.08.007","article-title":"A PiggyBac-mediated approach for muscle gene transfer or cell therapy","volume":"13","author":"Ley","year":"2014","journal-title":"Stem Cell Res."},{"key":"2021111618561100900_B69","doi-asserted-by":"crossref","first-page":"406","DOI":"10.2174\/156652311797415836","article-title":"Transposon-mediated gene transfer into adult and induced pluripotent stem cells","volume":"11","author":"Belay","year":"2011","journal-title":"Curr. Gene Ther."},{"key":"2021111618561100900_B70","doi-asserted-by":"crossref","first-page":"e104957","DOI":"10.1371\/journal.pone.0104957","article-title":"Delivery of full-length factor VIII using a piggyBac transposon vector to correct a mouse model of hemophilia A","volume":"9","author":"Matsui","year":"2014","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B71","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1002\/jgm.281","article-title":"High-level expression of naked DNA delivered to rat liver via tail vein injection","volume":"4","author":"Maruyama","year":"2002","journal-title":"J. Gene Med."},{"key":"2021111618561100900_B72","doi-asserted-by":"crossref","first-page":"2594","DOI":"10.1096\/fj.06-6254fje","article-title":"Sleeping Beauty-mediated eNOS gene therapy attenuates monocrotaline-induced pulmonary hypertension in rats","volume":"20","author":"Liu","year":"2006","journal-title":"FASEB J."},{"key":"2021111618561100900_B73","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1089\/10430340260395910","article-title":"Development of catheter-based procedures for transducing the isolated rabbit liver with plasmid DNA","volume":"13","author":"Eastman","year":"2002","journal-title":"Hum. Gene Ther."},{"key":"2021111618561100900_B74","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1038\/sj.gt.3302873","article-title":"Pig liver gene therapy by noninvasive interventionist catheterism","volume":"14","author":"Alino","year":"2007","journal-title":"Gene Ther."},{"key":"2021111618561100900_B75","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1089\/hum.2017.003","article-title":"Transgene expression in dogs after liver-directed hydrodynamic delivery of Sleeping Beauty transposons using balloon catheters","volume":"28","author":"Hyland","year":"2017","journal-title":"Hum. Gene Ther."},{"key":"2021111618561100900_B76","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1089\/hum.2017.004","article-title":"Prolonged expression of secreted enzymes in dogs after liver-directed delivery of Sleeping Beauty transposons: implications for non-viral gene therapy of systemic disease","volume":"28","author":"Aronovich","year":"2017","journal-title":"Hum. Gene Ther."},{"key":"2021111618561100900_B77","first-page":"2086","article-title":"Nanocapsule-delivered Sleeping Beauty mediates therapeutic Factor VIII expression in liver sinusoidal endothelial cells of hemophilia A mice","volume":"119","author":"Kren","year":"2009","journal-title":"J. Clin. Invest."},{"key":"2021111618561100900_B78","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/nnano.2017.57","article-title":"In situ programming of leukaemia-specific T cells using synthetic DNA nanocarriers","volume":"12","author":"Smith","year":"2017","journal-title":"Nat. Nanotechnol."},{"key":"2021111618561100900_B79","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.1038\/mt.2010.169","article-title":"Hyperactive sleeping beauty transposase enables persistent phenotypic correction in mice and a canine model for hemophilia B","volume":"18","author":"Hausl","year":"2010","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B80","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1038\/mt.2014.254","article-title":"Hybrid nonviral\/viral vector systems for improved piggyBac DNA transposon in vivo delivery","volume":"23","author":"Cooney","year":"2015","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B81","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1038\/nbt738","article-title":"Transposition from a gutless adeno-transposon vector stabilizes transgene expression in vivo","volume":"20","author":"Yant","year":"2002","journal-title":"Nat. Biotechnol."},{"key":"2021111618561100900_B82","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1089\/hum.2010.062","article-title":"Herpes simplex virus\/Sleeping Beauty vector-based embryonic gene transfer using the HSB5 mutant: loss of apparent transposition hyperactivity in vivo","volume":"21","author":"de Silva","year":"2010","journal-title":"Hum. Gene Ther."},{"key":"2021111618561100900_B83","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1038\/mt.2009.94","article-title":"Sleeping beauty transposition from nonintegrating lentivirus","volume":"17","author":"Vink","year":"2009","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B84","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1038\/mt.2009.10","article-title":"Hybrid lentivirus-transposon vectors with a random integration profile in human cells","volume":"17","author":"Staunstrup","year":"2009","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B85","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1038\/mt.2011.47","article-title":"Comparative genomic integration profiling of Sleeping Beauty transposons mobilized with high efficacy from integrase-defective lentiviral vectors in primary human cells","volume":"19","author":"Moldt","year":"2011","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B86","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0950-3536(98)80074-7","article-title":"Pathophysiology of sickle cell disease","volume":"11","author":"Steinberg","year":"1998","journal-title":"Baillieres Clin. Haematol."},{"key":"2021111618561100900_B87","doi-asserted-by":"crossref","first-page":"415","DOI":"10.3389\/fphys.2014.00415","article-title":"Haptoglobin, hemopexin, and related defense pathways-basic science, clinical perspectives, and drug development","volume":"5","author":"Schaer","year":"2014","journal-title":"Front. Physiol."},{"key":"2021111618561100900_B88","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/S0002-9440(10)64037-0","article-title":"Oxidative stress and induction of heme oxygenase-1 in the kidney in sickle cell disease","volume":"158","author":"Nath","year":"2001","journal-title":"Am. J. Pathol."},{"key":"2021111618561100900_B89","doi-asserted-by":"crossref","first-page":"18086","DOI":"10.1016\/S0021-9258(19)81326-1","article-title":"Mechanism of ferritin iron uptake: activity of the H-chain and deletion mapping of the ferro-oxidase site. A study of iron uptake and ferro-oxidase activity of human liver, recombinant H-chain ferritins, and of two H-chain deletion mutants","volume":"263","author":"Levi","year":"1988","journal-title":"J. Biol. Chem."},{"key":"2021111618561100900_B90","doi-asserted-by":"crossref","first-page":"2235","DOI":"10.1182\/blood.V122.21.2235.2235","article-title":"Determinants of heme-oxygenase-1 upregulation in patients with sickle cell disease","volume":"122","author":"Adisa","year":"2013","journal-title":"Blood"},{"key":"2021111618561100900_B91","doi-asserted-by":"crossref","first-page":"3649","DOI":"10.1182\/blood.V128.22.3649.3649","article-title":"Serum haptoglobin and hemopexin levels in pediatric SS and SC disease patients: biomarker of hemolysis and inflammation","volume":"128","author":"Santiago","year":"2016","journal-title":"Blood"},{"key":"2021111618561100900_B92","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1182\/blood-2009-03-210005","article-title":"Stable gene transfer and expression in cord blood-derived CD34+ hematopoietic stem and progenitor cells by a hyperactive Sleeping Beauty transposon system","volume":"114","author":"Xue","year":"2009","journal-title":"Blood"},{"key":"2021111618561100900_B93","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1089\/hum.2009.109","article-title":"Stable transgene expression in primitive human CD34+ hematopoietic stem\/progenitor cells, using the Sleeping Beauty transposon system","volume":"20","author":"Sumiyoshi","year":"2009","journal-title":"Hum. Gene Ther."},{"key":"2021111618561100900_B94","doi-asserted-by":"crossref","first-page":"e80403","DOI":"10.1371\/journal.pone.0080403","article-title":"beta-Globin sleeping beauty transposon reduces red blood cell sickling in a patient-derived CD34(+)-based in vitro model","volume":"8","author":"Sjeklocha","year":"2013","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B95","doi-asserted-by":"crossref","first-page":"e29110","DOI":"10.1371\/journal.pone.0029110","article-title":"Erythroid-specific expression of beta-globin from Sleeping Beauty-transduced human hematopoietic progenitor cells","volume":"6","author":"Sjeklocha","year":"2011","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B96","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1007\/s00109-010-0613-6","article-title":"Heme oxygenase-1 gene delivery by Sleeping Beauty inhibits vascular stasis in a murine model of sickle cell disease","volume":"88","author":"Belcher","year":"2010","journal-title":"J. Mol. Med. (Berl.)"},{"key":"2021111618561100900_B97","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2016.00063","article-title":"Hepatic overexpression of hemopexin inhibits inflammation and vascular stasis in murine models of sickle cell disease","volume":"22","author":"Vercellotti","year":"2016","journal-title":"Mol. Med."},{"key":"2021111618561100900_B98","doi-asserted-by":"crossref","first-page":"79","DOI":"10.3389\/fphar.2014.00079","article-title":"H-ferritin ferroxidase induces cytoprotective pathways and inhibits microvascular stasis in transgenic sickle mice","volume":"5","author":"Vercellotti","year":"2014","journal-title":"Front. Pharmacol."},{"key":"2021111618561100900_B99","doi-asserted-by":"crossref","first-page":"471325","DOI":"10.1155\/2012\/471325","article-title":"Glycosaminoglycan storage disorders: a review","volume":"2012","author":"Coutinho","year":"2012","journal-title":"Biochem. Res. Int."},{"key":"2021111618561100900_B100","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1517\/14712598.2010.531257","article-title":"Mesenchymal stem cells at the intersection of cell and gene therapy","volume":"10","author":"Myers","year":"2010","journal-title":"Expert Opin. Biol. Ther."},{"key":"2021111618561100900_B101","doi-asserted-by":"crossref","first-page":"e92420","DOI":"10.1371\/journal.pone.0092420","article-title":"Mesenchymal stem cells do not prevent antibody responses against human \u03b1-L-iduronidase when used to treat mucopolysaccharidosis type I","volume":"9","author":"Martin","year":"2014","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B102","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1002\/jgm.1028","article-title":"Prolonged expression of a lysosomal enzyme in mouse liver after Sleeping Beauty transposon-mediated gene delivery: implications for non-viral gene therapy of mucopolysaccharidoses","volume":"9","author":"Aronovich","year":"2007","journal-title":"J. Gene Med."},{"key":"2021111618561100900_B103","doi-asserted-by":"crossref","first-page":"e78161","DOI":"10.1371\/journal.pone.0078161","article-title":"Quantitative analysis of alpha-L-iduronidase expression in immunocompetent mice treated with the Sleeping Beauty transposon system","volume":"8","author":"Aronovich","year":"2013","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B104","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1038\/mt.2009.87","article-title":"Systemic correction of storage disease in MPS I NOD\/SCID mice using the sleeping beauty transposon system","volume":"17","author":"Aronovich","year":"2009","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B105","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1038\/sj.embor.7400221","article-title":"Duchenne muscular dystrophy and dystrophin: pathogenesis and opportunities for treatment","volume":"5","author":"Nowak","year":"2004","journal-title":"EMBO Rep."},{"key":"2021111618561100900_B106","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.ceb.2007.07.001","article-title":"Dysferlin and muscle membrane repair","volume":"19","author":"Han","year":"2007","journal-title":"Curr. Opin. Cell Biol."},{"key":"2021111618561100900_B107","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1038\/ejhg.2011.70","article-title":"Muscular dystrophy with marked Dysferlin deficiency is consistently caused by primary dysferlin gene mutations","volume":"19","author":"Cacciottolo","year":"2011","journal-title":"Eur. J. Hum. Genet."},{"key":"2021111618561100900_B108","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1152\/physrev.00043.2011","article-title":"Satellite cells and the muscle stem cell niche","volume":"93","author":"Yin","year":"2013","journal-title":"Physiol. Rev."},{"key":"2021111618561100900_B109","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s13395-015-0061-7","article-title":"Pax3-induced expansion enables the genetic correction of dystrophic satellite cells","volume":"5","author":"Filareto","year":"2015","journal-title":"Skelet. Muscle"},{"key":"2021111618561100900_B110","doi-asserted-by":"crossref","first-page":"e277","DOI":"10.1038\/mtna.2015.52","article-title":"Full-length dysferlin transfer by the hyperactive Sleeping Beauty transposase restores dysferlin-deficient muscle","volume":"5","author":"Escobar","year":"2016","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"2021111618561100900_B111","doi-asserted-by":"crossref","first-page":"2773","DOI":"10.1242\/dev.129.11.2773","article-title":"The meso-angioblast: a multipotent, self-renewing cell that originates from the dorsal aorta and differentiates into most mesodermal tissues","volume":"129","author":"Minasi","year":"2002","journal-title":"Development"},{"key":"2021111618561100900_B112","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1038\/nature05282","article-title":"Mesoangioblast stem cells ameliorate muscle function in dystrophic dogs","volume":"444","author":"Sampaolesi","year":"2006","journal-title":"Nature"},{"key":"2021111618561100900_B113","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s13395-015-0055-5","article-title":"Mesoangioblast delivery of miniagrin ameliorates murine model of merosin-deficient congenital muscular dystrophy type 1A","volume":"5","author":"Domi","year":"2015","journal-title":"Skelet. Muscle"},{"key":"2021111618561100900_B114","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1089\/hum.2012.088","article-title":"Recent progress in gene therapy for hemophilia","volume":"23","author":"Chuah","year":"2012","journal-title":"Hum. Gene Ther."},{"key":"2021111618561100900_B115","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/jth.12215","article-title":"Gene therapy for hemophilia","volume":"11","author":"Chuah","year":"2013","journal-title":"J. Thromb. Haemost."},{"key":"2021111618561100900_B116","doi-asserted-by":"crossref","first-page":"2691","DOI":"10.1182\/blood-2004-09-3496","article-title":"Phenotypic correction and long-term expression of factor VIII in hemophilic mice by immunotolerization and nonviral gene transfer using the Sleeping Beauty transposon system","volume":"105","author":"Ohlfest","year":"2005","journal-title":"Blood"},{"key":"2021111618561100900_B117","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1038\/gt.2009.13","article-title":"Indoleamine 2,3-dioxygenase attenuates inhibitor development in gene-therapy-treated hemophilia A mice","volume":"16","author":"Liu","year":"2009","journal-title":"Gene Ther."},{"key":"2021111618561100900_B118","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/S1525-0016(03)00216-8","article-title":"Helper-Independent Sleeping Beauty transposon-transposase vectors for efficient nonviral gene delivery and persistent gene expression in vivo","volume":"8","author":"Mikkelsen","year":"2003","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B119","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/75568","article-title":"Somatic integration and long-term transgene expression in normal and haemophilic mice using a DNA transposon system","volume":"25","author":"Yant","year":"2000","journal-title":"Nat. Genet."},{"key":"2021111618561100900_B120","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1016\/j.ymthe.2005.11.021","article-title":"Sustained FVIII expression and phenotypic correction of hemophilia A in neonatal mice using an endothelial-targeted sleeping beauty transposon","volume":"13","author":"Liu","year":"2006","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B121","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1038\/gt.2016.23","article-title":"Ultrasound-targeted hepatic delivery of factor IX in hemophiliac mice","volume":"23","author":"Anderson","year":"2016","journal-title":"Gene Ther."},{"key":"2021111618561100900_B122","doi-asserted-by":"crossref","first-page":"14042","DOI":"10.1038\/mtm.2014.42","article-title":"piggyBac-mediated phenotypic correction of factor VIII deficiency","volume":"1","author":"Staber","year":"2014","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"2021111618561100900_B123","doi-asserted-by":"crossref","first-page":"918471","DOI":"10.1155\/2011\/918471","article-title":"Tumor evasion from T cell surveillance","volume":"2011","author":"Topfer","year":"2011","journal-title":"J. Biomed. Biotechnol."},{"key":"2021111618561100900_B124","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.cell.2017.01.016","article-title":"The principles of engineering immune cells to treat cancer","volume":"168","author":"Lim","year":"2017","journal-title":"Cell"},{"key":"2021111618561100900_B125","doi-asserted-by":"crossref","first-page":"2961","DOI":"10.1158\/0008-5472.CAN-07-5600","article-title":"Redirecting specificity of T-cell populations for CD19 using the Sleeping Beauty system","volume":"68","author":"Singh","year":"2008","journal-title":"Cancer Res."},{"key":"2021111618561100900_B126","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1038\/gt.2011.40","article-title":"The hyperactive Sleeping Beauty transposase SB100X improves the genetic modification of T cells to express a chimeric antigen receptor","volume":"18","author":"Jin","year":"2011","journal-title":"Gene Ther."},{"key":"2021111618561100900_B127","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1038\/sj.mt.6300404","article-title":"Sleeping Beauty transposon-mediated engineering of human primary T cells for therapy of CD19+ lymphoid malignancies","volume":"16","author":"Huang","year":"2008","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B128","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1038\/mt.2010.141","article-title":"Gene transfer efficiency and genome-wide integration profiling of Sleeping Beauty, Tol2, and piggyBac transposons in human primary T cells","volume":"18","author":"Huang","year":"2010","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B129","doi-asserted-by":"crossref","first-page":"e0159477","DOI":"10.1371\/journal.pone.0159477","article-title":"Redirecting specificity of T cells using the Sleeping Beauty system to express chimeric antigen receptors by mix-and-matching of VL and VH domains targeting CD123+ tumors","volume":"11","author":"Thokala","year":"2016","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B130","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s13045-016-0256-3","article-title":"Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34(+) cells of juvenile myelomonocytic leukemia","volume":"9","author":"Nakazawa","year":"2016","journal-title":"J. Hematol. Oncol."},{"key":"2021111618561100900_B131","doi-asserted-by":"crossref","first-page":"2133","DOI":"10.1038\/mt.2011.131","article-title":"PiggyBac-mediated cancer immunotherapy using EBV-specific cytotoxic T-cells expressing HER2-specific chimeric antigen receptor","volume":"19","author":"Nakazawa","year":"2011","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B132","doi-asserted-by":"crossref","first-page":"1010428317695949","DOI":"10.1177\/1010428317695949","article-title":"Mesothelin-targeting chimeric antigen receptor-modified T cells by piggyBac transposon system suppress the growth of bile duct carcinoma","volume":"39","author":"Xu","year":"2017","journal-title":"Tumour Biol."},{"key":"2021111618561100900_B133","doi-asserted-by":"crossref","first-page":"e0133152","DOI":"10.1371\/journal.pone.0133152","article-title":"IGF1R- and ROR1-specific CAR T cells as a potential therapy for high risk sarcomas","volume":"10","author":"Huang","year":"2015","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B134","doi-asserted-by":"crossref","first-page":"e0128151","DOI":"10.1371\/journal.pone.0128151","article-title":"Sleeping Beauty transposition of chimeric antigen receptors targeting receptor tyrosine kinase-like orphan receptor-1 (ROR1) into diverse memory T-cell populations","volume":"10","author":"Deniger","year":"2015","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B135","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1158\/0008-5472.CAN-11-2778","article-title":"Genetically modified T cells targeting interleukin-11 receptor alpha-chain kill human osteosarcoma cells and induce the regression of established osteosarcoma lung metastases","volume":"72","author":"Huang","year":"2012","journal-title":"Cancer Res."},{"key":"2021111618561100900_B136","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.cell.2006.07.024","article-title":"Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors","volume":"126","author":"Takahashi","year":"2006","journal-title":"Cell"},{"key":"2021111618561100900_B137","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/j.bbrc.2014.06.014","article-title":"Non-viral reprogramming of fibroblasts into induced pluripotent stem cells by Sleeping Beauty and piggyBac transposons","volume":"450","author":"Talluri","year":"2014","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021111618561100900_B138","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1038\/nature07863","article-title":"piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells","volume":"458","author":"Woltjen","year":"2009","journal-title":"Nature"},{"key":"2021111618561100900_B139","doi-asserted-by":"crossref","first-page":"e0135403","DOI":"10.1371\/journal.pone.0135403","article-title":"Generation of na\u00efve bovine induced pluripotent stem cells using PiggyBac transposition of doxycycline-inducible transcription factors","volume":"10","author":"Kawaguchi","year":"2015","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B140","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1089\/scd.2012.0382","article-title":"Derivation and characterization of sleeping beauty transposon-mediated porcine induced pluripotent stem cells","volume":"22","author":"Kues","year":"2013","journal-title":"Stem Cells Dev."},{"key":"2021111618561100900_B141","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1089\/cell.2014.0080","article-title":"Derivation and characterization of bovine induced pluripotent stem cells by transposon-mediated reprogramming","volume":"17","author":"Talluri","year":"2015","journal-title":"Cell Reprogram."},{"key":"2021111618561100900_B142","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.diff.2013.06.002","article-title":"Generation of induced pluripotent stem cells from human foetal fibroblasts using the Sleeping Beauty transposon gene delivery system","volume":"86","author":"Davis","year":"2013","journal-title":"Differentiation"},{"key":"2021111618561100900_B143","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1093\/nar\/gks1305","article-title":"Sleeping Beauty transposon-based system for cellular reprogramming and targeted gene insertion in induced pluripotent stem cells","volume":"41","author":"Grabundzija","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"2021111618561100900_B144","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/nprot.2016.152","article-title":"Efficient, footprint-free human iPSC genome editing by consolidation of Cas9\/CRISPR and piggyBac technologies","volume":"12","author":"Wang","year":"2017","journal-title":"Nat. Protoc."},{"key":"2021111618561100900_B145","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/978-1-61779-201-4_7","article-title":"Transgene-free production of pluripotent stem cells using piggyBac transposons","volume":"767","author":"Woltjen","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"2021111618561100900_B146","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1002\/bit.25018","article-title":"Seamless correction of the sickle cell disease mutation of the HBB gene in human induced pluripotent stem cells using TALENs","volume":"111","author":"Sun","year":"2014","journal-title":"Biotechnol. Bioeng."},{"key":"2021111618561100900_B147","doi-asserted-by":"crossref","first-page":"1526","DOI":"10.1101\/gr.173427.114","article-title":"Seamless gene correction of beta-thalassemia mutations in patient-specific iPSCs using CRISPR\/Cas9 and piggyBac","volume":"24","author":"Xie","year":"2014","journal-title":"Genome Res."},{"key":"2021111618561100900_B148","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.stemcr.2017.01.022","article-title":"Reversal of phenotypic abnormalities by CRISPR\/Cas9-mediated gene correction in Huntington disease patient-derived induced pluripotent stem cells","volume":"8","author":"Xu","year":"2017","journal-title":"Stem Cell Rep."},{"key":"2021111618561100900_B149","doi-asserted-by":"crossref","first-page":"3363","DOI":"10.1172\/JCI86721","article-title":"Phase I trials using Sleeping Beauty to generate CD19-specific CAR T cells","volume":"126","author":"Kebriaei","year":"2016","journal-title":"J. Clin. Invest."},{"key":"2021111618561100900_B150","doi-asserted-by":"crossref","first-page":"e64138","DOI":"10.1371\/journal.pone.0064138","article-title":"Manufacture of clinical-grade CD19-specific T cells stably expressing chimeric antigen receptor using Sleeping Beauty system and artificial antigen presenting cells","volume":"8","author":"Singh","year":"2013","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B151","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1038\/leu.2015.247","article-title":"Chimeric antigen receptor-modified T cells derived from defined CD8+ and CD4+ subsets confer superior antitumor reactivity in vivo","volume":"30","author":"Sommermeyer","year":"2016","journal-title":"Leukemia"},{"key":"2021111618561100900_B152","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.1172\/JCI85309","article-title":"CD19 CAR\u2013T cells of defined CD4+:CD8+ composition in adult B cell ALL patients","volume":"126","author":"Turtle","year":"2016","journal-title":"J. Clin. Invest."},{"key":"2021111618561100900_B153","doi-asserted-by":"crossref","first-page":"E7788","DOI":"10.1073\/pnas.1610544113","article-title":"Tethered IL-15 augments antitumor activity and promotes a stem-cell memory subset in tumor-specific T cells","volume":"113","author":"Hurton","year":"2016","journal-title":"Proc. Natl Acad. Sci. U.S.A."},{"key":"2021111618561100900_B154"},{"key":"2021111618561100900_B155","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/S0022-2836(02)00991-9","article-title":"Common physical properties of DNA affecting target site selection of sleeping beauty and other Tc1\/mariner transposable elements","volume":"323","author":"Vigdal","year":"2002","journal-title":"J. Mol. Biol."},{"key":"2021111618561100900_B156","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1097\/CJI.0b013e3181b2914c","article-title":"Genome-wide mapping of PiggyBac transposon integrations in primary human T cells","volume":"32","author":"Galvan","year":"2009","journal-title":"J. Immunother."},{"key":"2021111618561100900_B157","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1186\/1471-2164-10-418","article-title":"Genome-wide analysis of Tol2 transposon reintegration in zebrafish","volume":"10","author":"Kondrychyn","year":"2009","journal-title":"BMC Genomics"},{"key":"2021111618561100900_B158","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1038\/mt.2016.11","article-title":"Genome-wide profiling reveals remarkable parallels between insertion site selection properties of the MLV retrovirus and the piggyBac transposon in primary human CD4+ T cells","volume":"24","author":"Gogol-Doring","year":"2016","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B159","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/sj.mt.6300366","article-title":"Transcriptional activities of the Sleeping Beauty transposon and shielding its genetic cargo with insulators","volume":"16","author":"Walisko","year":"2008","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B160","doi-asserted-by":"crossref","first-page":"e85494","DOI":"10.1371\/journal.pone.0085494","article-title":"piggyBac transposon plus insulators overcome epigenetic silencing to provide for stable signaling pathway reporter cell lines","volume":"8","author":"Mossine","year":"2013","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B161","doi-asserted-by":"crossref","first-page":"e48421","DOI":"10.1371\/journal.pone.0048421","article-title":"The impact of cHS4 insulators on DNA transposon vector mobilization and silencing in retinal pigment epithelium cells","volume":"7","author":"Sharma","year":"2012","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B162","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1038\/mt.2008.224","article-title":"Shielding of sleeping beauty DNA transposon-delivered transgene cassettes by heterologous insulators in early embryonal cells","volume":"17","author":"Dalsgaard","year":"2009","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B163","doi-asserted-by":"crossref","first-page":"1636","DOI":"10.1038\/mt.2011.129","article-title":"Manipulating piggyBac transposon chromosomal integration site selection in human cells","volume":"19","author":"Kettlun","year":"2011","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B164","doi-asserted-by":"crossref","first-page":"6978","DOI":"10.1093\/nar\/gks309","article-title":"Chimeric piggyBac transposases for genomic targeting in human cells","volume":"40","author":"Owens","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"2021111618561100900_B165","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1080\/10409238.2016.1237935","article-title":"Sleeping Beauty transposition: from biology to applications","volume":"52","author":"Narayanavari","year":"2017","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"2021111618561100900_B166","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/S0168-9525(99)01777-1","article-title":"Resident aliens: the Tc1\/mariner superfamily of transposable elements","volume":"15","author":"Plasterk","year":"1999","journal-title":"Trends Genet."},{"key":"2021111618561100900_B167","doi-asserted-by":"crossref","first-page":"7884","DOI":"10.1073\/pnas.1104208108","article-title":"The catalytic domain of all eukaryotic cut-and-paste transposase superfamilies","volume":"108","author":"Yuan","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021111618561100900_B168","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/1471-2199-9-73","article-title":"Mutational analysis of highly conserved aspartate residues essential to the catalytic core of the piggyBac transposase","volume":"9","author":"Keith","year":"2008","journal-title":"BMC Mol. Biol."},{"key":"2021111618561100900_B169","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/1471-2199-9-72","article-title":"Analysis of the piggyBac transposase reveals a functional nuclear targeting signal in the 94 c-terminal residues","volume":"9","author":"Keith","year":"2008","journal-title":"BMC Mol. Biol."},{"key":"2021111618561100900_B170","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1002\/jgm.486","article-title":"Excision of Sleeping Beauty transposons: parameters and applications to gene therapy","volume":"6","author":"Liu","year":"2004","journal-title":"J. Gene Med."},{"key":"2021111618561100900_B171","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/j.1365-2583.1996.tb00048.x","article-title":"Precise excision of TTAA-specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera","volume":"5","author":"Fraser","year":"1996","journal-title":"Insect Mol. Biol."},{"key":"2021111618561100900_B172","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1002\/dvdy.20157","article-title":"Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo","volume":"231","author":"Parinov","year":"2004","journal-title":"Dev. Dyn."},{"key":"2021111618561100900_B173","doi-asserted-by":"crossref","first-page":"e36","DOI":"10.1093\/nar\/gks1035","article-title":"Genomic deletion induced by Tol2 transposon excision in zebrafish","volume":"41","author":"Huang","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"2021111618561100900_B174","first-page":"828","article-title":"piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice","volume":"6","author":"Li","year":"2013","journal-title":"Dis. Model Mech."},{"key":"2021111618561100900_B175","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1002\/dvg.20508","article-title":"Chromosomal mobilization and reintegration of Sleeping Beauty and PiggyBac transposons","volume":"47","author":"Liang","year":"2009","journal-title":"Genesis"},{"key":"2021111618561100900_B176","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1038\/nmeth795","article-title":"Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system","volume":"2","author":"Keng","year":"2005","journal-title":"Nat. Methods"},{"key":"2021111618561100900_B177","doi-asserted-by":"crossref","first-page":"9189","DOI":"10.1128\/MCB.23.24.9189-9207.2003","article-title":"Characterization of Sleeping Beauty transposition and its application to genetic screening in mice","volume":"23","author":"Horie","year":"2003","journal-title":"Mol. Cell. Biol."},{"key":"2021111618561100900_B178","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1128\/MCB.00670-12","article-title":"The piggyBac transposon displays local and distant reintegration preferences and can cause mutations at noncanonical integration sites","volume":"33","author":"Li","year":"2013","journal-title":"Mol. Cell. Biol."},{"key":"2021111618561100900_B179","doi-asserted-by":"crossref","first-page":"9290","DOI":"10.1073\/pnas.0801017105","article-title":"Chromosomal transposition of PiggyBac in mouse embryonic stem cells","volume":"105","author":"Wang","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021111618561100900_B180","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1093\/genetics\/149.1.179","article-title":"Factors affecting transposition of the Himar1 mariner transposon in vitro","volume":"149","author":"Lampe","year":"1998","journal-title":"Genetics"},{"key":"2021111618561100900_B181","doi-asserted-by":"crossref","first-page":"3153","DOI":"10.1038\/nprot.2007.471","article-title":"Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection","volume":"2","author":"Bell","year":"2007","journal-title":"Nat. Protoc."},{"key":"2021111618561100900_B182","doi-asserted-by":"crossref","first-page":"e75344","DOI":"10.1371\/journal.pone.0075344","article-title":"Integration profile and safety of an adenovirus hybrid-vector utilizing hyperactive sleeping beauty transposase for somatic integration","volume":"8","author":"Zhang","year":"2013","journal-title":"PLoS ONE"},{"key":"2021111618561100900_B183","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1038\/sj.gt.3301978","article-title":"Sustainable correction of junctional epidermolysis bullosa via transposon-mediated nonviral gene transfer","volume":"10","author":"Ortiz-Urda","year":"2003","journal-title":"Gene Ther."},{"key":"2021111618561100900_B184","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1271\/bbb.80581","article-title":"Sleeping Beauty-mediated suicide gene therapy of hepatocellular carcinoma","volume":"73","author":"Song","year":"2009","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"2021111618561100900_B185","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.jid.2016.11.038","article-title":"Correction of recessive dystrophic epidermolysis bullosa by transposon-mediated integration of COL7A1 in transplantable patient-derived primary keratinocytes","volume":"137","author":"Latella","year":"2017","journal-title":"J. Invest. Dermatol."},{"key":"2021111618561100900_B186","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.bbrc.2005.02.024","article-title":"Sleeping Beauty-mediated down-regulation of huntingtin expression by RNA interference","volume":"329","author":"Chen","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021111618561100900_B187","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1002\/jgm.1589","article-title":"Sleeping Beauty-mediated correction of Fanconi anemia type C","volume":"13","author":"Hyland","year":"2011","journal-title":"J. Gene Med."},{"key":"2021111618561100900_B188","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s13195-016-0195-9","article-title":"Targeted delivery of nerve growth factor to the cholinergic basal forebrain of Alzheimer\u2019s disease patients: application of a second-generation encapsulated cell biodelivery device","volume":"8","author":"Eyjolfsdottir","year":"2016","journal-title":"Alzheimers Res. Ther."},{"key":"2021111618561100900_B189","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1038\/gt.2011.178","article-title":"Increased encapsulated cell biodelivery of nerve growth factor in the brain by transposon-mediated gene transfer","volume":"19","author":"Fjord-Larsen","year":"2012","journal-title":"Gene Ther."},{"key":"2021111618561100900_B190","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1038\/sj.cgt.7701045","article-title":"Transposon-based interferon gamma gene transfer overcomes limitations of episomal plasmid for immunogene therapy of glioblastoma","volume":"14","author":"Wu","year":"2007","journal-title":"Cancer Gene Ther."},{"key":"2021111618561100900_B191","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1476-4598-10-14","article-title":"Inhibition of angiogenesis and suppression of colorectal cancer metastatic to the liver using the Sleeping Beauty Transposon System","volume":"10","author":"Belur","year":"2011","journal-title":"Mol. Cancer"},{"key":"2021111618561100900_B192","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1002\/hep.23060","article-title":"Long-term reduction of jaundice in Gunn rats by nonviral liver-targeted delivery of Sleeping Beauty transposon","volume":"50","author":"Wang","year":"2009","journal-title":"Hepatology"},{"key":"2021111618561100900_B193","doi-asserted-by":"crossref","first-page":"567","DOI":"10.3748\/wjg.v10.i4.567","article-title":"Insulin expression in livers of diabetic mice mediated by hydrodynamics-based administration","volume":"10","author":"He","year":"2004","journal-title":"World J. Gastroenterol."},{"key":"2021111618561100900_B194","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1038\/mt.2015.221","article-title":"Sleeping Beauty transposon vectors in liver-directed gene delivery of LDLR and VLDLR for gene therapy of familial hypercholesterolemia","volume":"24","author":"Turunen","year":"2016","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B195","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/75568","article-title":"Somatic integration and long-term transgene expression in normal and haemophilic mice using a DNA transposon system","volume":"25","author":"Yant","year":"2000","journal-title":"Nat. Genet."},{"key":"2021111618561100900_B196","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1161\/ATVBAHA.116.308680","article-title":"Long-Term prevention of congenital thrombotic thrombocytopenic purpura in ADAMTS13 knockout mice by Sleeping Beauty transposon-mediated gene therapy","volume":"37","author":"Verhenne","year":"2017","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"2021111618561100900_B197","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1006\/mthe.2002.0812","article-title":"In vivo correction of murine tyrosinemia type I by DNA-mediated transposition","volume":"6","author":"Montini","year":"2002","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B198","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1177\/030006051204000523","article-title":"Sleeping Beauty transposon system is a reliable gene delivery tool for hereditary tyrosinaemia type 1 disease gene therapy: size of the foreign gene decides the timing of stable integration into the host chromosomes","volume":"40","author":"Pan","year":"2012","journal-title":"J. Int. Med. Res."},{"key":"2021111618561100900_B199","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1038\/sj.mt.6300160","article-title":"Messenger RNA as a source of transposase for sleeping beauty transposon-mediated correction of hereditary tyrosinemia type I","volume":"15","author":"Wilber","year":"2007","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B200","doi-asserted-by":"crossref","first-page":"2384","DOI":"10.1096\/fj.06-6228fje","article-title":"Sleeping Beauty-based gene therapy with indoleamine 2,3-dioxygenase inhibits lung allograft fibrosis","volume":"20","author":"Liu","year":"2006","journal-title":"FASEB J."},{"key":"2021111618561100900_B201","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1038\/mt.2012.36","article-title":"miR-29 inhibits bleomycin-induced pulmonary fibrosis in mice","volume":"20","author":"Xiao","year":"2012","journal-title":"Mol. Ther."},{"key":"2021111618561100900_B202","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1164\/rccm.201304-0700OC","article-title":"Endothelial indoleamine 2,3-dioxygenase protects against development of pulmonary hypertension","volume":"188","author":"Xiao","year":"2013","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"2021111618561100900_B203","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1038\/nature10424","article-title":"Targeted gene correction of alpha1-antitrypsin deficiency in induced pluripotent stem cells","volume":"478","author":"Yusa","year":"2011","journal-title":"Nature"},{"key":"2021111618561100900_B204","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1089\/hum.2009.114","article-title":"piggyBac transposon\/transposase system to generate CD19-specific T cells for the treatment of B-lineage malignancies","volume":"21","author":"Manuri","year":"2010","journal-title":"Hum. Gene Ther."},{"key":"2021111618561100900_B205","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1016\/j.jcyt.2015.05.013","article-title":"Low-cost generation of Good Manufacturing Practice-grade CD19-specific chimeric antigen receptor-expressing T cells using piggyBac gene transfer and patient-derived materials","volume":"17","author":"Ramanayake","year":"2015","journal-title":"Cytotherapy"},{"key":"2021111618561100900_B206","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1016\/j.jcyt.2014.05.022","article-title":"Anti-leukemic potency of piggyBac-mediated CD19-specific T cells against refractory Philadelphia chromosome-positive acute lymphoblastic leukemia","volume":"16","author":"Saito","year":"2014","journal-title":"Cytotherapy"},{"key":"2021111618561100900_B207","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1016\/j.ajpath.2015.01.027","article-title":"Protective role of insulin-like growth factor-1 receptor in endothelial cells against unilateral ureteral obstruction-induced renal fibrosis","volume":"185","author":"Liang","year":"2015","journal-title":"Am. J. Pathol."},{"key":"2021111618561100900_B208","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1002\/path.4067","article-title":"Loss of glutathione S-transferase A4 accelerates obstruction-induced tubule damage and renal fibrosis","volume":"228","author":"Liang","year":"2012","journal-title":"J. Pathol."},{"key":"2021111618561100900_B209","doi-asserted-by":"crossref","first-page":"3511","DOI":"10.1182\/blood.V128.22.3511.3511","article-title":"Long-term expression of von Willebrand factor via Piggybac-mediated gene transfer","volume":"128","author":"Johnson","year":"2016","journal-title":"Blood"},{"key":"2021111618561100900_B210","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1186\/1471-2407-9-126","article-title":"Tumor-directed gene therapy in mice using a composite nonviral gene delivery system consisting of the piggyBac transposon and polyethylenimine","volume":"9","author":"Kang","year":"2009","journal-title":"BMC Cancer"},{"key":"2021111618561100900_B211","first-page":"897","article-title":"High-level transgene expression mediated by the piggyBac transposon enhances transgenic therapeutic effects in cervical cancer xenografts","volume":"24","author":"Kang","year":"2010","journal-title":"Oncol. Rep."}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20160614\/807611\/bsr-2016-0614c.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20160614\/807611\/bsr-2016-0614c.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T14:38:07Z","timestamp":1637073487000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/37\/6\/BSR20160614\/57599\/Preclinical-and-clinical-advances-in-transposon"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,5]]},"references-count":211,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,12,22]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20160614","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,5]]},"article-number":"BSR20160614"}}