{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T15:51:22Z","timestamp":1773676282826,"version":"3.50.1"},"reference-count":33,"publisher":"Portland Press Ltd.","issue":"6","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":609,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,12,22]]},"abstract":"<jats:p>Severe acute pancreatitis (SAP) remains to be challenging for its unpredictable inflammatory progression from acute pancreatitis to SAP. Apoptosis is an important pathology of SAP. Fibrinogen-like protein 2 (FGL2) has been reported to be involved in apoptosis. The present study aimed to explore the therapeutic effect of an adenovirus-mediated artificial miRNA targetting FGL2 (Ad-FGL2-miRNA) in taurocholate-induced murine pancreatitis models. Sodium taurocholate was retrogradely injected into the biliopancreatic ducts of the C57\/BL mice to induce SAP. FGL2 expression was measured with reverse transcription-PCR, Western blotting, and immunohistochemical staining. ELISA was used to detect the activity of amylase and the concentrations of tumor necrosis factor-\u03b1 (TNF-\u03b1) and interleukin-1\u03b2 (IL-1\u03b2). In addition, the mRNA levels of TNF-\u03b1 and IL-1\u03b2 were also detected. Finally, apoptosis was assessed by terminal deoxynucleotidyl transferase mediated dUTP-biotin nick-end labeling (TUNEL) method and Western blotting. Ad-FGL2-miRNA significantly suppressed FGL2 expression and alleviated pancreatic injury. Also, Ad-FGL2-miRNA markedly inhibited a post-SAP increase in the activation of TNF-\u03b1 and IL-1\u03b2. Finally, pretreatment with Ad-FGL2-miRNA ameliorated apoptosis at the early stage of SAP by modulating cleaved caspase-3 and therefore played a protective role. These results indicated that FGL2 might be a promising target for attenuating the severity of SAP and adenovirus-mediated artificial miRNAs targetting FGL2 represented a potential therapeutic approach for the treatment of SAP.<\/jats:p>","DOI":"10.1042\/bsr20170964","type":"journal-article","created":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T15:47:27Z","timestamp":1508773647000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Adenovirus-mediated artificial miRNA targetting fibrinogen-like protein 2 attenuates the severity of acute pancreatitis in mice"],"prefix":"10.1042","volume":"37","author":[{"given":"Xiaohua","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Jinhua Hospital of Zhejiang University, Jinhua 321000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Jinhua Hospital of Zhejiang University, Jinhua 321000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanping","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Jinhua Hospital of Zhejiang University, Jinhua 321000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiayue","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Jinhua Hospital of Zhejiang University, Jinhua 321000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2017,11,23]]},"reference":[{"key":"2021111618592634300_B1","doi-asserted-by":"crossref","first-page":"e2685","DOI":"10.1038\/cddis.2017.70","article-title":"Deletion of XIAP reduces the severity of acute pancreatitis via regulation of cell death and nuclear factor-kappaB activity","volume":"8","author":"Liu","year":"2017","journal-title":"Cell Death Dis."},{"key":"2021111618592634300_B2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biopha.2016.04.048","article-title":"Ligustrazine alleviates acute pancreatitis by accelerating acinar cell apoptosis at early phase via the suppression of p38 and Erk MAPK pathways","volume":"82","author":"Chen","year":"2016","journal-title":"Biomed. 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