{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T19:25:28Z","timestamp":1777145128993,"version":"3.51.4"},"reference-count":36,"publisher":"Portland Press Ltd.","issue":"1","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":561,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,2,28]]},"abstract":"<jats:p>MicroRNAs (miRNAs) play an essential role in articular cartilage development and growth. However, the exact mechanisms involved in this process remain unknown. In the present study, we investigated the biological functions of miR-27b during hypertrophic differentiation of rat articular chondrocytes. Based on in situ hybridization and immunohistochemistry, we report that miR-27b expression is reduced in the hypertrophic zone of articular cartilage, but expression of peroxisome proliferator-activated receptor \u03b3 (Ppar\u03b3) is increased. Dual-luciferase reporter gene assay and Western blot analysis demonstrated that Ppar\u03b32 is a target of miR-27b. Overexpression of miR-27b inhibited expression of Ppar\u03b32, as well as type X collagen (Col10a1) and matrix metalloproteinase 13 (Mmp13), while significantly promoting the expression of Sex-determining Region-box 9 (Sox9) and type II collagen (Col2a1) at both the mRNA and protein levels. Rosiglitazone, a Ppar\u03b3 agonist, suppressed Col2a1 expression, while promoting expression of runt-related transcription factor 2 (Runx2) and Col10a1 in a concentration-dependent manner. siRNA-mediated knockdown of Ppar\u03b32 caused an increase in protein levels of Col2a1. The present study demonstrates that miR-27b regulates chondrocyte hypertrophy in part by targetting Ppar\u03b32, and that miR-27b may have important therapeutic implications in cartilage diseases.<\/jats:p>","DOI":"10.1042\/bsr20171109","type":"journal-article","created":{"date-parts":[[2017,11,30]],"date-time":"2017-11-30T01:10:17Z","timestamp":1512004217000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["<i>miR-27b<\/i> promotes type II collagen expression by targetting peroxisome proliferator-activated receptor-\u03b32 during rat articular chondrocyte differentiation"],"prefix":"10.1042","volume":"38","author":[{"given":"Jinying","family":"Xu","sequence":"first","affiliation":[{"name":"The Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130000, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Lv","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130000, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Regeneration Medicine, School of Pharmaceutical Science of Jilin University, Changchun 130000, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, The First Hospital of Jilin University, Changchun 130000, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongjie","family":"Sun","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130000, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangyang","family":"Zheng","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130000, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zechuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Clinical Medicine College, Jilin University, Changchun 130000, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianglan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology, China-Japan Union Hospital, Jilin University, Changchun 130000, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulin","family":"Li","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130000, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangfan","family":"Chi","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130000, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2018,1,10]]},"reference":[{"key":"2021111618493033900_B1","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1038\/nature01657","article-title":"Developmental regulation of the growth plate","volume":"423","author":"Kronenberg","year":"2003","journal-title":"Nature"},{"key":"2021111618493033900_B2","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1146\/annurev.genom.9.081307.164437","article-title":"Transcriptional control of skeletogenesis","volume":"9","author":"Karsenty","year":"2008","journal-title":"Annu. 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