{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T03:27:19Z","timestamp":1761017239683,"version":"3.41.2"},"reference-count":50,"publisher":"Portland Press Ltd.","issue":"6","license":[{"start":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T00:00:00Z","timestamp":1564099200000},"content-version":"vor","delay-in-days":261,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,12,21]]},"abstract":"<jats:p>Objectives: Acute coronary syndrome (ACS) is the major cause of mortality worldwide and caused mainly by atherosclerosis of coronary arteries. Apolipoprotein B100 (ApoB100) is a major component of low-density lipoprotein (LDL) and its oxidation can trigger inflammation in vascular endothelial cells leading to atherosclerosis. The association between antibodies to ApoB100-derived antigens and atherosclerotic diseases has been studied in recent years, but the findings appear to be controversial. The present study developed an ELISA in-house with ApoB100-derived peptide antigens to circulating anti-ApoB100 IgG antibodies in patients with ACS. Methods: Fifteen ApoB100-derived peptide antigens (Ag1\u2013Ag15) were designed to develop an in-house ELISA for the detection of circulating anti-ApoB100 IgG levels in 350 patients with ACS and 201 control subjects amongst a Chinese population. Binary logistic regression was applied to examine the differences in anti-ApoB IgG levels between the patient group and the control group with adjustment for a number of confounding factors; the correlation between anti-ApoB100 IgG levels and clinical characteristics was also tested. Results: Patients with ACS had significantly higher levels of plasma IgG for Ag1 (adjusted P&amp;lt;0.001) and Ag10 antigens (adjusted P&amp;lt;0.001). There was no significant increase in the levels of IgG to the other 13 antigens in these ACS patients. In the control group, anti-Ag10 IgG levels were positively correlated with age, high-density lipoprotein (HDL), and ApoA levels (P\u22640.001 for all) and negatively correlated with blood triglyceride (TG) (P=0.008); in the patient group, anti-Ag10 IgG levels were positively correlated with LDL (P=0.003), and negatively correlated with ApoA (P=0.048) and systolic blood pressure (SBP) (P=0.036). The area under ROC (receiver operator characteristic) curve (AUC) was 0.612 (95% confidence interval (CI): 0.560\u20130.664; P&amp;lt;0.001) in anti-Ag1 IgG assay and 0.621 (95% CI: 0.569\u20130.672; P&amp;lt;0.001) in anti-Ag10 IgG assay. Conclusion: Circulating IgG for ApoB100-derived peptide antigens may be a useful biomarker of ACS, although anti-ApoB IgG levels were not associated with the coronary artery plaque burden characterized by the coronary Gensini score.<\/jats:p>","DOI":"10.1042\/bsr20180450","type":"journal-article","created":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T15:33:16Z","timestamp":1537543996000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Associations between circulating IgG antibodies to Apolipoprotein B100-derived peptide antigens and acute coronary syndrome in a Chinese Han population"],"prefix":"10.1042","volume":"38","author":[{"given":"Weina","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Medicine, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan East Road, Shenyang 110032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan East Road, Shenyang 110032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunan","family":"Han","sequence":"additional","affiliation":[{"name":"Division of Public Health Sciences, Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan East Road, Shenyang 110032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingming","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan East Road, Shenyang 110032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian\u00a0L.","family":"Megson","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Cardiovascular Science, University of the Highlands and Islands, Centre for Health Science, Inverness IV2 3JH, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Cardiovascular Science, University of the Highlands and Islands, Centre for Health Science, Inverness IV2 3JH, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4546-2607","authenticated-orcid":false,"given":"Yuanzhe","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The Fourth Affiliated Hospital of China Medical University, No. 4 Chongshan East Road, Shenyang 110032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2018,11,7]]},"reference":[{"key":"2021111619200004600_B1","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.ijcard.2018.05.134","article-title":"Predictive value of the age, creatinine, and ejection fraction (ACEF) score in patients with acute coronary syndromes","volume":"270","author":"Stahli","year":"2018","journal-title":"Int. J. Cardiol."},{"key":"2021111619200004600_B2","first-page":"1","article-title":"Reports of cardiovascular diseases in China at 2017","volume":"33","author":"Chen weiw","year":"2017","journal-title":"China Circ. J."},{"key":"2021111619200004600_B3","doi-asserted-by":"publisher","first-page":"2427","DOI":"10.1016\/j.jacc.2012.08.969","article-title":"ACCF 2012 expert consensus document on practical clinical considerations in the interpretation of troponin elevations: a report of the American College of Cardiology Foundation task force on Clinical Expert Consensus Documents","volume":"60","author":"Newby","year":"2012","journal-title":"J. Am. Coll. Cardiol."},{"key":"2021111619200004600_B4","doi-asserted-by":"publisher","first-page":"454","DOI":"10.1590\/S1679-45082015RW3172","article-title":"Acute management of unstable angina and non-ST segment elevation myocardial infarction","volume":"13","author":"Silva","year":"2015","journal-title":"Einstein"},{"key":"2021111619200004600_B5","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/j.hlc.2013.03.001","article-title":"Pathophysiology of atherosclerosis plaque progression","volume":"22","author":"Sakakura","year":"2013","journal-title":"Heart Lung Circ."},{"key":"2021111619200004600_B6","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1111\/j.1365-2796.2008.01945.x","article-title":"Autoimmunity in atherosclerosis: a protective response losing control?","volume":"263","author":"Nilsson","year":"2008","journal-title":"J. Intern. Med."},{"key":"2021111619200004600_B7","doi-asserted-by":"publisher","first-page":"S56","DOI":"10.1007\/s10875-010-9396-3","article-title":"Natural IgM antibodies against oxidation-specific epitopes","volume":"30","author":"Binder","year":"2010","journal-title":"J. Clin. Immunol."},{"key":"2021111619200004600_B8","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1038\/ni.2001","article-title":"The immune system in atherosclerosis","volume":"12","author":"Hansson","year":"2011","journal-title":"Nat. Immunol."},{"key":"2021111619200004600_B9","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1080\/00365510701842996","article-title":"Oxidized LDL and anti-oxLDL antibody levels in peripheral atherosclerotic disease","volume":"68","author":"Andican","year":"2008","journal-title":"Scand. J. Clin. Lab. Invest."},{"key":"2021111619200004600_B10","doi-asserted-by":"crossref","first-page":"131","DOI":"10.33549\/physiolres.930043","article-title":"Antibodies against oxidized LDL\u2013theory and clinical use","volume":"50","author":"Steinerova","year":"2001","journal-title":"Physiol. Res."},{"key":"2021111619200004600_B11","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1007\/s000110300074","article-title":"Increased microvascular permeability in the hamster cheek pouch induced by oxidized low density lipoprotein (oxLDL) and some fragmented apolipoprotein B proteins","volume":"52","author":"Svensjo","year":"2003","journal-title":"Inflamm. Res."},{"key":"2021111619200004600_B12","doi-asserted-by":"publisher","first-page":"877","DOI":"10.1161\/01.ATV.0000209886.31510.7f","article-title":"Persistent high levels of plasma oxidized low-density lipoprotein after acute myocardial infarction predict stent restenosis","volume":"26","author":"Naruko","year":"2006","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"2021111619200004600_B13","first-page":"499","article-title":"Lipoprotein autoantibodies: measurement and significance","volume":"10","author":"Virella","year":"2003","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"2021111619200004600_B14","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/S0022-2143(99)90050-1","article-title":"Lipid peroxidation and atherosclerosis in type II diabetes","volume":"134","author":"Oranje","year":"1999","journal-title":"J. Lab. Clin. Med."},{"key":"2021111619200004600_B15","first-page":"815","article-title":"Direct evidence for a protein recognized by a monoclonal antibody against oxidatively modified LDL in atherosclerotic lesions from a Watanabe heritable hyperlipidemic rabbit","volume":"135","author":"Boyd","year":"1989","journal-title":"Am. J. Pathol."},{"key":"2021111619200004600_B16","doi-asserted-by":"publisher","first-page":"2552","DOI":"10.1161\/01.CIR.0000143225.07377.EA","article-title":"Are anti-oxidized low-density lipoprotein antibodies pathogenic or protective?","volume":"110","author":"Shoenfeld","year":"2004","journal-title":"Circulation"},{"key":"2021111619200004600_B17","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1196\/annals.1361.077","article-title":"Systemic lupus erythematosus, atherosclerosis, and autoantibodies","volume":"1051","author":"Zampieri","year":"2005","journal-title":"Ann. N.Y. Acad. Sci."},{"key":"2021111619200004600_B18","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1177\/1479164112443374","article-title":"Adaptive immunity is related to coronary artery disease severity after acute coronary syndrome in subjects with metabolic syndrome","volume":"10","author":"Izar","year":"2013","journal-title":"Diabetes Vasc. Dis. Res."},{"key":"2021111619200004600_B19","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1186\/1476-511X-13-111","article-title":"Multivessel coronary artery disease, free fatty acids, oxidized LDL and its antibody in myocardial infarction","volume":"13","author":"Gruzdeva","year":"2014","journal-title":"Lipids Health Dis."},{"key":"2021111619200004600_B20","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1590\/S0066-782X2010005000063","article-title":"Antibodies against oxLDL and acute coronary syndrome","volume":"95","author":"Medeiros","year":"2010","journal-title":"Arq. Bras. Cardiol."},{"key":"2021111619200004600_B21","first-page":"845960","article-title":"Investigation of serum oxidized low-density lipoprotein IgG levels in patients with angiographically defined coronary artery disease","volume":"2014","author":"Moohebati","year":"2014","journal-title":"Int. J. Vasc. Med."},{"key":"2021111619200004600_B22","doi-asserted-by":"publisher","first-page":"e68039","DOI":"10.1371\/journal.pone.0068039","article-title":"Passive immunization with hypochlorite-oxLDL specific antibodies reduces plaque volume in LDL receptor-deficient mice","volume":"8","author":"van Leeuwen","year":"2013","journal-title":"PLoS ONE"},{"key":"2021111619200004600_B23","first-page":"821","article-title":"Antibodies to oxidized low density lipoprotein: epidemiological studies and potential clinical applications in cardiovascular disease","volume":"55","author":"Gounopoulos","year":"2007","journal-title":"Minerva Cardioangiol."},{"key":"2021111619200004600_B24","doi-asserted-by":"publisher","first-page":"1092","DOI":"10.1016\/j.immuni.2013.06.009","article-title":"Immune effector mechanisms implicated in atherosclerosis: from mice to humans","volume":"38","author":"Libby","year":"2013","journal-title":"Immunity"},{"key":"2021111619200004600_B25","doi-asserted-by":"publisher","first-page":"872","DOI":"10.1161\/01.ATV.0000067935.02679.B0","article-title":"Identification of immune responses against aldehyde-modified peptide sequences in apoB associated with cardiovascular disease","volume":"23","author":"Fredrikson","year":"2003","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"2021111619200004600_B26","doi-asserted-by":"crossref","first-page":"136","DOI":"10.3109\/14017431.2015.1023344","article-title":"A study of IgG antibodies to the ApoB protein in non-ST segment elevation acute coronary syndrome","volume":"49","author":"Zhang","year":"2015","journal-title":"Scand. Cardiovasc. J."},{"key":"2021111619200004600_B27","doi-asserted-by":"publisher","first-page":"712","DOI":"10.1016\/j.fob.2015.08.006","article-title":"Detection of circulating IgG antibodies to apolipoprotein B100 in acute myocardial infarction","volume":"5","author":"Zhang","year":"2015","journal-title":"FEBS Open Bio"},{"key":"2021111619200004600_B28","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1046\/j.1365-2796.2000.00656.x","article-title":"Immune responses to oxidative neoepitopes on LDL and phospholipids modulate the development of atherosclerosis","volume":"247","author":"Palinski","year":"2000","journal-title":"J. Intern. Med."},{"key":"2021111619200004600_B29","doi-asserted-by":"publisher","first-page":"2713","DOI":"10.1016\/j.jacc.2014.09.016","article-title":"2014 AHA\/ACC guideline for the management of patients with non-st-elevation acute coronary syndromes: a report of the american college of cardiology\/american heart association task force on practice guidelines","volume":"64","author":"Amsterdam","year":"2014","journal-title":"J. Am. Coll. Cardiol."},{"key":"2021111619200004600_B30","doi-asserted-by":"publisher","first-page":"1581","DOI":"10.1016\/j.jacc.2012.08.001","article-title":"Third universal definition of myocardial infarction","volume":"60","author":"Thygesen","year":"2012","journal-title":"J. Am. Coll. Cardiol."},{"key":"2021111619200004600_B31","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1016\/S0002-9149(83)80105-2","article-title":"A more meaningful scoring system for determining the severity of coronary heart disease","volume":"51","author":"Gensini","year":"1983","journal-title":"Am. J. Cardiol."},{"key":"2021111619200004600_B32","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1186\/1471-2105-11-568","article-title":"Peptide binding predictions for HLA DR, DP and DQ molecules","volume":"11","author":"Sidnay","year":"2010","journal-title":"BMC Bioinformatics"},{"key":"2021111619200004600_B33","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1016\/j.it.2011.07.001","article-title":"How dendritic cells shape atherosclerosis","volume":"32","author":"Koltsova","year":"2011","journal-title":"Trends Immunol."},{"key":"2021111619200004600_B34","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1172\/JCI63108","article-title":"Adaptive immunity in atherogenesis: new insights and therapeutic approaches","volume":"123","author":"Lichtman","year":"2013","journal-title":"J. Clin. Invest."},{"key":"2021111619200004600_B35","doi-asserted-by":"publisher","first-page":"2311","DOI":"10.1161\/ATVBAHA.108.179697","article-title":"Oxidized low-density lipoprotein and atherosclerosis","volume":"30","author":"Steinberg","year":"2010","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"2021111619200004600_B36","first-page":"383","article-title":"Serum antibodies to oxidized low-density lipoprotein and ceroid in chronic periaortitis","volume":"114","author":"Parums","year":"1990","journal-title":"Arch. Pathol. Lab. Med."},{"key":"2021111619200004600_B37","doi-asserted-by":"publisher","first-page":"152786","DOI":"10.1155\/2013\/152786","article-title":"LOX-1, OxLDL, and atherosclerosis","volume":"2013","author":"Pirillo","year":"2013","journal-title":"Mediators Inflamm."},{"key":"2021111619200004600_B38","doi-asserted-by":"crossref","first-page":"417","DOI":"10.2459\/JCM.0b013e3283638d79","article-title":"Oxidized low-density lipoprotein antibodies in myocardial infarction patients without classical risk factors","volume":"15","author":"Gomez","year":"2013","journal-title":"J. Cardiovasc. Med. (Hagerstown)"},{"key":"2021111619200004600_B39","doi-asserted-by":"publisher","first-page":"e0143102","DOI":"10.1371\/journal.pone.0143102","article-title":"Associations of circulating oxidized LDL and conventional biomarkers of cardiovascular disease in a cross-sectional study of the navajo population","volume":"11","author":"Harmon","year":"2016","journal-title":"PLoS ONE"},{"key":"2021111619200004600_B40","doi-asserted-by":"publisher","first-page":"1250","DOI":"10.1161\/01.ATV.0000166518.96137.38","article-title":"Humoral immune response against defined oxidized low-density lipoprotein antigens reflects structure and disease activity of carotid plaques","volume":"25","author":"Goncalves","year":"2005","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"2021111619200004600_B41","doi-asserted-by":"publisher","first-page":"1426","DOI":"10.1007\/s00125-009-1377-9","article-title":"Associations between autoantibodies against apolipoprotein B-100 peptides and vascular complications in patients with type 2 diabetes","volume":"52","author":"Fredrikson","year":"2009","journal-title":"Diabetologia"},{"key":"2021111619200004600_B42","doi-asserted-by":"publisher","first-page":"e188","DOI":"10.1016\/j.atherosclerosis.2006.12.014","article-title":"Autoantibody against the amino acid sequence 661-680 in apo B-100 is associated with decreased carotid stenosis and cardiovascular events","volume":"194","author":"Fredrikson","year":"2007","journal-title":"Atherosclerosis"},{"key":"2021111619200004600_B43","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1016\/j.atherosclerosis.2013.11.041","article-title":"Plasma autoantibodies against apolipoprotein B-100 peptide 210 in subclinical atherosclerosis","volume":"232","author":"McLeod","year":"2014","journal-title":"Atherosclerosis"},{"key":"2021111619200004600_B44","doi-asserted-by":"publisher","first-page":"e0120744","DOI":"10.1371\/journal.pone.0120744","article-title":"Circulating autoantibodies against the apolipoprotein B-100 peptides p45 and p210 in relation to the occurrence of carotid plaques in 64-year-old women","volume":"10","author":"Fagerberg","year":"2015","journal-title":"PLoS ONE"},{"key":"2021111619200004600_B45","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1016\/j.atherosclerosis.2015.01.023","article-title":"Low levels of IgG autoantibodies against the apolipoprotein B antigen p210 increases the risk of cardiovascular death after carotid endarterectomy","volume":"239","author":"Asciutto","year":"2015","journal-title":"Atherosclerosis"},{"key":"2021111619200004600_B46","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1111\/cei.12651","article-title":"Decreased levels of autoantibodies against apolipoprotein B-100 antigens are associated with cardiovascular disease in systemic lupus erythematosus","volume":"181","author":"Svenungsson","year":"2015","journal-title":"Clin. Exp. Immunol."},{"key":"2021111619200004600_B47","doi-asserted-by":"publisher","first-page":"2120","DOI":"10.1001\/jama.2011.1654","article-title":"Number of coronary heart disease risk factors and mortality in patients with first myocardial infarction","volume":"306","author":"Canto","year":"2011","journal-title":"JAMA"},{"key":"2021111619200004600_B48","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1177\/0961203311398884","article-title":"Assessment of IgG antibodies to oxidized LDL in patients with acute coronary syndrome","volume":"20","author":"Laczik","year":"2011","journal-title":"Lupus"},{"key":"2021111619200004600_B49","doi-asserted-by":"publisher","first-page":"2218","DOI":"10.1093\/eurheartj\/ehn336","article-title":"High plasma concentrations of autoantibodies against native peptide 210 of apoB-100 are related to less coronary atherosclerosis and lower risk of myocardial infarction","volume":"29","author":"Sjogren","year":"2008","journal-title":"Eur. Heart J."},{"key":"2021111619200004600_B50","doi-asserted-by":"publisher","first-page":"2209","DOI":"10.1016\/j.jacc.2014.01.061","article-title":"Distinct morphological features of ruptured culprit plaque for acute coronary events compared to those with silent rupture and thin-cap fibroatheroma: a combined optical coherence tomography and intravascular ultrasound study","volume":"63","author":"Tian","year":"2014","journal-title":"J. Am. Coll. Cardiol."}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20180450\/809287\/bsr-2018-0450.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20180450\/809287\/bsr-2018-0450.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T16:20:46Z","timestamp":1720628446000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/38\/6\/BSR20180450\/98170\/Associations-between-circulating-IgG-antibodies-to"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,7]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,12,21]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20180450","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"type":"print","value":"0144-8463"},{"type":"electronic","value":"1573-4935"}],"subject":[],"published":{"date-parts":[[2018,11,7]]},"article-number":"BSR20180450"}}