{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T11:05:47Z","timestamp":1777892747787,"version":"3.51.4"},"reference-count":30,"publisher":"Portland Press Ltd.","issue":"4","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":359,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,8,31]]},"abstract":"<jats:p>The study\u2019s aim was to image severe alcoholic hepatitis (SAH) using 111In-labelled leucocytes with two objectives in mind: firstly for non-invasive diagnosis and secondly to provide a platform for experimental therapies aiming to inhibit intrahepatic neutrophil migration. 111In-leucocyte scintigraphy was performed 30 min and 24 h post-injection in 19 patients with SAH, 14 abstinent patients with alcohol-related cirrhosis and 11 normal controls. Eleven with SAH and seven with cirrhosis also had 99mTc-nanocolloid scintigraphy. Change in hepatic 111In radioactivity was expressed as decay-corrected 24 h:30 min count ratio and, in SAH, compared with histological grading of steatohepatitis and expression of granulocyte marker, CD15. Hepatic microautoradiography on biopsy specimens obtained 24 h post-injection of 111In-leucocytes was performed in one patient. Median 24 h:30 min hepatic 111In activity ratio was higher in SAH (2.5 (interquartile range (IQR): 1.7\u20134.0) compared with cirrhotics and normal controls (1.0 (0.8\u20131.1) and 0.8 (0.7\u20130.9) respectively, P&amp;lt;0.0001). In SAH, it correlated with CD15 expression (r = 0.62, P=0.023) and was higher in marked compared with mild\/moderate steatohepatitis (4.0 (3.0\u20134.6) compared with 1.8 (1.5\u20132.6), P=0.006). Hepatic-to-splenic 99mTc count rate ratio was reduced in SAH (0.5 (0.4\u20131.4)) compared with cirrhotics (2.3( 0.6\u20133.0)) and three historic normal controls (4.2 (3.8\u20135.0); P=0.003), consistent with impaired hepatic reticuloendothelial function. Scintigraphic findings in SAH included prominent lung radioactivity at 30 min, likely the result of neutrophil primimg. Microautoradiography demonstrated cell-associated 111In in areas of parenchymal neutrophil infiltration. In conclusion, 111In-leucocyte scintigraphy can non-invasively diagnose SAH and could provide a platform for evaluation of novel treatments aiming to inhibit intrahepatic neutrophil migration.<\/jats:p>","DOI":"10.1042\/bsr20180466","type":"journal-article","created":{"date-parts":[[2018,4,26]],"date-time":"2018-04-26T17:00:16Z","timestamp":1524762016000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<i>In vivo<\/i> imaging of hepatic neutrophil migration in severe alcoholic hepatitis with 111In-radiolabelled leucocytes"],"prefix":"10.1042","volume":"38","author":[{"given":"Jonathan\u00a0R.","family":"Potts","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Brighton and Sussex University Hospitals NHS Trust, Brighton, U.K."},{"name":"Department of Medicine, Brighton and Sussex Medical School, Brighton, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neda","family":"Farahi","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Cambridge School of Clinical Medicine, Cambridge, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark\u00a0R.","family":"Howard","sequence":"additional","affiliation":[{"name":"Department of Histopathology, Brighton and Sussex University Hospitals NHS Trust, Brighton, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark\u00a0R.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Histopathology, Brighton and Sussex University Hospitals NHS Trust, Brighton, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Heard","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, Cambridge University Hospitals NHS Foundation Trust, Cambridge, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arun\u00a0N.","family":"Shankar","sequence":"additional","affiliation":[{"name":"Department of Hepatology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graeme\u00a0J.","family":"Alexander","sequence":"additional","affiliation":[{"name":"Department of Hepatology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edwin\u00a0R.","family":"Chilvers","sequence":"additional","affiliation":[{"name":"Department of Histopathology, Brighton and Sussex University Hospitals NHS Trust, Brighton, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumita","family":"Verma","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Brighton and Sussex University Hospitals NHS Trust, Brighton, U.K."},{"name":"Department of Medicine, Brighton and Sussex Medical School, Brighton, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.\u00a0Michael","family":"Peters","sequence":"additional","affiliation":[{"name":"Division of Clinical and Laboratory Investigation, Brighton and Sussex Medical School, Brighton, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2018,7,31]]},"reference":[{"key":"2021111618593751400_B1","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1016\/S0140-6736(12)60244-X","article-title":"Projections of alcohol-related deaths in England and Wales\u2013tragic toll or potential prize?","volume":"379","author":"Sheron","year":"2012","journal-title":"Lancet"},{"key":"2021111618593751400_B2","doi-asserted-by":"publisher","first-page":"685","DOI":"10.7326\/0003-4819-110-9-685","article-title":"Methylprednisolone therapy in patients with severe alcoholic hepatitis. 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