{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:44:12Z","timestamp":1777445052583,"version":"3.51.4"},"reference-count":29,"publisher":"Portland Press Ltd.","issue":"1","license":[{"start":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T00:00:00Z","timestamp":1563408000000},"content-version":"vor","delay-in-days":198,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,1,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Expression of hypoxia-inducible factor (HIF) 1\u03b1 has been observed in corneal neovascularization (CNV). Vascular endothelial growth factor (VEGF), one of the most well-known angiogenic factors in CNV, is under the regulation of HIF-1. The present study aims to investigate the synergistic effects of VEGF and HIF-1\u03b1 gene silencing on alkali burn-induced CNV in rabbits. The models of rabbits in corneal alkali burn were established. SiRNA recombinant adenovirus was used to explore the synergistic effects of VEGF and HIF-1\u03b1 gene silencing on alkali burn-induced CNV. CNV area and ultrastructure of cornea were observed. The expression of VEGF and HIF-1\u03b1 was detected. CNV was observed in rabbits following alkali burn. In addition, overexpressed VEGF and HIF-1\u03b1 was also observed in rabbits following alkali burn. Then, silencing HIF-1\u03b1 or silencing VEGF decreased area of CNV, inhibited neovascularization and improved pathological changes, while double-target interference for VEGF and HIF-1\u03b1 decreased area of CNV inhibited neovascularization, and improved pathological changes to a greater extent. Our study provides evidences emphasizing the distinct notion that VEGF and HIF-1\u03b1 play the contributory role in alkali burn-induced CNV as a result of double-target interference for VEGF and HIF-1\u03b1 inhibiting CNV in rabbits following corneal alkali burn.<\/jats:p>","DOI":"10.1042\/bsr20180552","type":"journal-article","created":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T22:45:48Z","timestamp":1540421148000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Inhibited corneal neovascularization in rabbits following corneal alkali burn by double-target interference for VEGF and HIF-1\u03b1"],"prefix":"10.1042","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0876-1384","authenticated-orcid":false,"given":"Ying-Cong","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Burns and Plastic Surgery, Shengli Oilfield Central Hospital, Dongying 257034, P.R. 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