{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T09:07:35Z","timestamp":1778663255075,"version":"3.51.4"},"reference-count":49,"publisher":"Portland Press Ltd.","issue":"5","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":309,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,10,31]]},"abstract":"<jats:p>We aim to investigate the function and mechanism of GanDouLing combinated with Penicillamine on cerebrovascular injury in Wilson\u2019s disease (WD). ELISA was performed to analyze the expression of vascular injury factors. Pathological changes of cerebral vessels were observed by HE stain. Immunohistochemistry assays were performed to analyze the expression of ICAM-1, VCAM-1, and GRP78. Western blotting was measured to analyze the expression of caspase-3, caspase-12, PERK, eIF2\u03b1, and CHOP. Apoptosis was detected with TUNEL assay. The expression of vascular injury factors and ICAM-1, VCAM-1 was significantly increased by WD and markedly decreased in GanDouLing-Penicillamine group. The expression of caspase-3, caspase-12, PERK, eIF2\u03b1, and CHOP were obviously expressed in Wilson group, GanDouLing-Penicillamine suppressed apoptosis and endoplasmic reticulum (ER) stress. Our findings suggested that GanDouLing-Penicillamine improved cerebrovascular injury through PERK\/eIF2\u03b1\/CHOP ER stress pathway in the mouse model of WD.<\/jats:p>","DOI":"10.1042\/bsr20180800","type":"journal-article","created":{"date-parts":[[2018,9,4]],"date-time":"2018-09-04T13:46:03Z","timestamp":1536068763000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["GanDouLing combined with Penicillamine improves cerebrovascular injury via PERK\/eIF2\u03b1\/CHOP endoplasmic reticulum stress pathway in the mouse model of Wilson\u2019s disease"],"prefix":"10.1042","volume":"38","author":[{"given":"Yonghua","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Neurology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, China"},{"name":"Graduate Institute, Anhui University of Chinese Medicine, Hefei, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shijian","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Neurology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ni","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neurology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenming","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Neurology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2018,9,19]]},"reference":[{"key":"2021111619143974800_B1","doi-asserted-by":"publisher","first-page":"1997","DOI":"10.1093\/brain\/awp193","article-title":"Progressive lenticular degeneration: a familial nervous disease associated with cirrhosis of the liver, by S. 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