{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T14:15:16Z","timestamp":1781187316727,"version":"3.54.1"},"reference-count":39,"publisher":"Portland Press Ltd.","issue":"1","license":[{"start":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T00:00:00Z","timestamp":1563408000000},"content-version":"vor","delay-in-days":198,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,1,31]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Amoebiasis caused by the protozoan parasite Entamoeba histolytica remains a public health problem in developing countries, making the identification of new anti-amoebic compounds a continuing priority. Previously, we have shown that lactoferrin (Lf) and several Lf-derived peptides exhibit in vitro anti-amoebic activity independently of their iron-binding activity. Here, we evaluated the amoebicidal effect of synthetic Lf-derived peptides Lfcin-B, Lfcin 17-30, and Lfampin, analyzed the mechanism of death induced by the peptides and determined their therapeutic effects on murine intestinal amoebiasis. MTT assays in trophozoite cultures of E. histolytica exposed to each peptide (1\u20131000 \u03bcM) showed that Lfampin is far more amoebicidal than Lfcins. Lfampin killed 80% of trophozoites at doses higher than 100 \u03bcM in 24 h, and FACs analysis using Annexin V\/propidium iodide showed that death occurred mainly by necrosis. In contrast, Lfcin-B and Lfcin 17-30 appeared to have no significant effect on amoebic viability. FACs and confocal microscopy analysis using FITC-labeled peptides showed that all three peptides are internalized by the amoeba mainly using receptor (PI3K signaling) and actin-dependent pathways but independent of clathrin. Docking studies identified cholesterol in the amoeba\u2019s plasma membrane as a possible target of Lfampin. Oral treatment of intracecally infected mice with the abovementioned peptides at 10 mg\/kg for 4 days showed that Lfampin resolved 100% of the cases of intestinal amoebiasis, whereas Lfcin 17-30 and Lfcin-B were effective in resolving infection in 80 and 70% of cases, respectively. These data show that although synthetic bovine Lf-derived peptides exhibit varying amoebicidal potentials in vitro, they do resolve murine intestinal amoebiasis efficiently, suggesting that they may be useful as a therapeutic treatment.<\/jats:p>","DOI":"10.1042\/bsr20180850","type":"journal-article","created":{"date-parts":[[2018,11,15]],"date-time":"2018-11-15T09:56:15Z","timestamp":1542275775000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Synthetic bovine lactoferrin peptide Lfampin kills<i>Entamoeba histolytica<\/i>trophozoites by necrosis and resolves amoebic intracecal infection in mice"],"prefix":"10.1042","volume":"39","author":[{"given":"C\u00e9sar","family":"D\u00edaz-God\u00ednez","sequence":"first","affiliation":[{"name":"Department of Immunology, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, 04510, M\u00e9xico, D.F., M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ximena","family":"Gonz\u00e1lez-Galindo","sequence":"additional","affiliation":[{"name":"Department of Immunology, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, 04510, M\u00e9xico, D.F., M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thuluz","family":"Meza-Menchaca","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00f3mica Humana, Facultad de Medicina, Universidad Veracruzana, Calle M\u00e9dicos y Odont\u00f3logos S\/N., Col. Unidad del Bosque, 91010, Xalapa, Veracruz, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal\u00a0J.","family":"Bobes","sequence":"additional","affiliation":[{"name":"Department of Immunology, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, 04510, M\u00e9xico, D.F., M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireya","family":"de la Garza","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology, Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN, Av. IPN 2508, San Pedro Zacatenco, 07360 M\u00e9xico, D.F., M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidia","family":"Le\u00f3n-Sicairos","sequence":"additional","affiliation":[{"name":"CIASaP, Facultad de Medicina, Universidad Aut\u00f3noma de Sinaloa, Cedros y Sauces, Fracc. Fresnos Culiac\u00e1n, 80246, Sinaloa, M\u00e9xico"},{"name":"Departamento f Research, Hospital Pedi\u00e1trico de Sinaloa, Boulevard Constituci\u00f3n S\/N, Col. Jorge Almada, Culiacan, 80200, Sinaloa, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan P.","family":"Laclette","sequence":"additional","affiliation":[{"name":"Department of Immunology, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, 04510, M\u00e9xico, D.F., M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1055-5774","authenticated-orcid":false,"given":"Julio\u00a0C.","family":"Carrero","sequence":"additional","affiliation":[{"name":"Department of Immunology, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, 04510, M\u00e9xico, D.F., M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2019,1,8]]},"reference":[{"key":"2021111617022240800_B1","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1093\/clinids\/8.2.228","article-title":"Problems in recognition and diagnosis of amebiasis: estimation of the global magnitude of morbidity and mortality","volume":"8","author":"Walsh","year":"1986","journal-title":"Rev. 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