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Skin samples were collected from 18 patients with psoriasis vulgaris and 9 healthy participants for RNA sequencing. Candidate genes were chosen based on degree and k-core difference of genes in the co-expression network. The relations between candidate genes and CD274 were validated by flow cytometry and real-time PCR in primary human epidermal keratinocytes. The therapeutic effect of indirubin was assessed in an imiquimod-treated mouse model. Interferon-\u03b3 (IFN-\u03b3), cyclin-dependent kinase (CDK) 1, Toll-like receptor 3 (TLR3), TLR4 and interleukin (IL)-17A were considered as candidate genes. In primary human epidermal keratinocytes, the level of CD274 was obviously increased under the stimulation of IFN-\u03b3 and CDK1 inhibitor (indirubin), independent of TLR4, TLR3 or IL-17A. Indirubin alleviated the severity of psoriatic mice in a CD274-dependent manner. Co-expression network analysis served as an effective method for the exploration of molecular mechanisms. We demonstrated for the first time that CD274 was the regulator of indirubin-mediated effect on mouse psoriasis-like skin lesion based on co-expression network analysis, contributing to the alleviation of mouse psoriasis-like skin lesion.<\/jats:p>","DOI":"10.1042\/bsr20180958","type":"journal-article","created":{"date-parts":[[2018,10,19]],"date-time":"2018-10-19T15:15:18Z","timestamp":1539962118000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Indirubin attenuates mouse psoriasis-like skin lesion in a CD274-dependent manner: an achievement of RNA sequencing"],"prefix":"10.1042","volume":"38","author":[{"given":"Xiaochun","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China"},{"name":"Department of Pharmacy, the 85th Hospital of PLA, Shanghai 200052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhua","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junhui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianguo","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiying","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Congshan","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Wuxi Maternal and Child Health-Care Hospital, Wuxi 214000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9218-4673","authenticated-orcid":false,"given":"Jinhong","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2018,11,23]]},"reference":[{"key":"2021111619244718900_B1","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1016\/S0140-6736(14)61909-7","article-title":"Psoriasis","volume":"386","author":"Boehncke","year":"2015","journal-title":"Lancet North Am. 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