{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T11:59:16Z","timestamp":1772884756933,"version":"3.50.1"},"reference-count":32,"publisher":"Portland Press Ltd.","issue":"6","license":[{"start":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T00:00:00Z","timestamp":1564099200000},"content-version":"vor","delay-in-days":231,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,12,21]]},"abstract":"<jats:p>Human cytomegalovirus (HCMV) infection can cause inflammatory tissue-invasive end-organ diseases upon lytic replication. In humans, mature miR-200b-3p and -200c-3p suppress the synthesis of HCMV immediate early 2 (IE2) protein by binding to the 3\u2032-UTR of the mRNA encoded by the unique long (UL) 122-123 region in human foreskin fibroblasts and pre-transplant peripheral blood mononuclear cells stimulated with HCMV. The present study aimed to quantitate the expression of Homo sapiens (hsa)-miR-200b-3p and 200c-3p in HCMV-infected tissues. We collected 240 HCMV-infected and 154 HCMV-non-infected, formalin-fixed, paraffin-embedded tissue samples of the gastrointestinal (GI) tract and bronchi\/lungs. MiRNAs, HCMV, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were quantitated by quantitative reverse transcription-PCR (qRT-PCR) and quantitative PCR (qPCR) on the basis of standard curves generated using miRNA mimics, the HCMV strain from National Institute for Biological Standards and Control (NIBSC) 09\/162, and GAPDH control. To avoid the effect of cell counts on the qRT-PCR and qPCR results, the data were normalized to GAPDH levels. HCMV-infected tissues had significantly lower levels of 200b-3p\/GAPDH (3.03 \u00b1 1.50 compared with 3.98 \u00b1 1.08 log10 copies\/\u03bcl, P&amp;lt;0.001) and 200c-3p\/GAPDH (4.67 \u00b1 1.84 compared with 6.35 \u00b1 1.47 log10 copies\/\u03bcl, P&amp;lt;0.001) than normal tissues. The values for 200b-3p\/GAPDH (r = \u22120.51, P&amp;lt;0.001) and 200c-3p\/GAPDH (r = \u22120.54, P&amp;lt;0.001) were significantly inversely correlated with HCMV load. Low tissue levels of 200b-3p and 200c-3p in humans are associated with cytopathic inflammation due to HCMV infection.<\/jats:p>","DOI":"10.1042\/bsr20180961","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T21:44:21Z","timestamp":1540590261000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Expression of human miR-200b-3p and -200c-3p in cytomegalovirus-infected tissues"],"prefix":"10.1042","volume":"38","author":[{"given":"Kyoung\u00a0Hwa","family":"Lee","sequence":"first","affiliation":[{"name":"Division of Infectious Disease, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beom\u00a0Jin","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor\u00a0H.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Multi-Organ Transplant Program, University Health Network, Toronto, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seo\u00a0Yeon","family":"Min","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeon-Mi","family":"Hong","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong-Hyeon","family":"Jo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4278-5198","authenticated-orcid":false,"given":"Sang\u00a0Hoon","family":"Han","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2018,12,7]]},"reference":[{"key":"2021111619184550300_B1","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1111\/ajt.12103","article-title":"Cytomegalovirus in solid organ transplantation","volume":"13","author":"Razonable","year":"2013","journal-title":"Am. 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