{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T16:47:41Z","timestamp":1768927661102,"version":"3.49.0"},"reference-count":49,"publisher":"Portland Press Ltd.","issue":"6","license":[{"start":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T00:00:00Z","timestamp":1564099200000},"content-version":"vor","delay-in-days":247,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,12,21]]},"abstract":"<jats:p>Obstructive cholestasis characterized by biliary pressure increase leading to leakage of bile back that causes liver injury. The present study aims to evaluate the effects of artemisinin in obstructive cholestasis in mice. The present study was carried out on 40 adult healthy mice that were divided into 4 groups, 10 mice each; the negative control group didn\u2019t receive any medication. The normal group was fed normally with 100 mg\/kg of artemisinin extract orally. The cholestatic group fed on 1% lithocholic acid (LCA) mixed into control diet and cholestatic group co-treated with 100 mg\/kg of artemisinin extract orally. Mice were treated for 1 month then killed at end of the experiment. A significant increase in alanine aminotransferase, aspartate aminotransferase, and total and direct bilirubin was detected in mice exposed to LCA toxicity. That increase was significantly reduced to normal values in mice co-treated with artemisinin. LCA toxicity causes multiple areas of necrosis of irregular distribution. However, artemisinin co-treatment showed normal hepatic architecture. Moreover, LCA causes down-regulation of hepatic mRNA expressions of a set of genes that are responsible for ATP binding cassette and anions permeability as ATP-binding cassette sub-family G member 8, organic anion-transporting polypeptide, and multidrug resistance-associated protein 2 genes that were ameliorated by artemisinin administration. Similarly, LCA toxicity significantly down-regulated hepatic mRNA expression of constitutive androstane receptor, OATP4, and farnesoid x receptor genes. However, artemisinin treatment showed a reasonable prevention. In conclusion, the current study strikingly revealed that artemisinin treatment can prevent severe hepatotoxicity and cholestasis that led via LCA exposure.<\/jats:p>","DOI":"10.1042\/bsr20181011","type":"journal-article","created":{"date-parts":[[2018,9,16]],"date-time":"2018-09-16T00:15:32Z","timestamp":1537056932000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Preventive effect of artemisinin extract against cholestasis induced via lithocholic acid exposure"],"prefix":"10.1042","volume":"38","author":[{"given":"Adel\u00a0Qlayel","family":"Alkhedaide","sequence":"first","affiliation":[{"name":"Medical Laboratory Department, Faculty of Applied Medical Sciences, Turabah, Taif University, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamer\u00a0Ahmed","family":"Ismail","sequence":"additional","affiliation":[{"name":"Medical Laboratory Department, Faculty of Applied Medical Sciences, Turabah, Taif University, Saudi Arabia"},{"name":"Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saad\u00a0Hmoud","family":"Alotaibi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Applied Medical Sciences, Turabah, Taif University, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2806-9136","authenticated-orcid":false,"given":"Mohamed\u00a0Abdo","family":"Nassan","sequence":"additional","affiliation":[{"name":"Medical Laboratory Department, Faculty of Applied Medical Sciences, Turabah, Taif University, Saudi Arabia"},{"name":"Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zafer Saad\u00a0Al","family":"Shehri","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Department, Al-Dawadmi College of Applied Medical Sciences, Shaqra University, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2018,11,21]]},"reference":[{"key":"2021111619215302900_B1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.cld.2007.11.010","article-title":"Mechanisms of cholestasis","volume":"12","author":"Zollner","year":"2008","journal-title":"Clin. 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