{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T11:03:32Z","timestamp":1788001412645,"version":"build-2784847793"},"reference-count":90,"publisher":"Portland Press Ltd.","issue":"3","license":[{"start":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:00:00Z","timestamp":1567987200000},"content-version":"vor","delay-in-days":192,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,3,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>It is difficult to determinate the cause of death from exposure to fatal hypothermia and hyperthermia in forensic casework. Here, we present a state-of-the-art study that employs Fourier-transform infrared (FTIR) spectroscopy to investigate the hypothalamus tissues of fatal hypothermic, fatal hyperthermic and normothermic rats to determine forensically significant biomarkers related to fatal hypothermia and hyperthermia. Our results revealed that the spectral variations in the lipid, protein, carbohydrate and nucleic acid components are highly different for hypothalamuses after exposure to fatal hypothermic, fatal hyperthermic and normothermic conditions. In comparison with the normothermia group, the fatal hypothermia and hyperthermia groups contained higher total lipid amounts but were lower in unsaturated lipids. Additionally, their cell membranes were found to have less motional freedom. Among these three groups, the fatal hyperthermia group contained the lowest total proteins and carbohydrates and the highest aggregated and dysfunctional proteins, while the fatal hypothermia group contained the highest level of nucleic acids. In conclusion, this study demonstrates that FTIR spectroscopy has the potential to become a reliable method for the biochemical characterization of fatal hypothermia and hyperthermia hypothalamus tissues, and this could be used as a postmortem diagnostic feature in fatal hypothermia and hyperthermia deaths.<\/jats:p>","DOI":"10.1042\/bsr20181633","type":"journal-article","created":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T11:44:13Z","timestamp":1551440653000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Biochemical detection of fatal hypothermia and hyperthermia in affected rat hypothalamus tissues by Fourier transform infrared spectroscopy"],"prefix":"10.1042","volume":"39","author":[{"given":"Hancheng","family":"Lin","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China"},{"name":"Department of Forensic Pathology, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaifei","family":"Deng","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"Luo","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiran","family":"Sun","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengdong","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijiu","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Forensic Pathology, Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9344-9215","authenticated-orcid":false,"given":"Ping","family":"Huang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2019,3,15]]},"reference":[{"key":"2021111619313990700_B1","doi-asserted-by":"publisher","first-page":"603","DOI":"10.4065\/78.5.603","article-title":"Skin blood flow in adult human thermoregulation: how it works, when it does not, and why","volume":"78","author":"Charkoudian","year":"2003","journal-title":"Mayo Clin. Proc."},{"key":"2021111619313990700_B2","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1007\/s00414-012-0722-6","article-title":"Hypothalamus tissues of fatal hypothermic, fatal hyperthermic and normothermic rats","volume":"127","author":"Palmiere","year":"2013","journal-title":"Int. J. Legal Med."},{"key":"2021111619313990700_B3","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1007\/s00414-014-0977-1","article-title":"Postmortem diagnosis of hypothermia","volume":"128","author":"Palmiere","year":"2014","journal-title":"Int. J. Legal Med."},{"key":"2021111619313990700_B4","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1007\/s00414-012-0738-y","article-title":"Postmortem biochemical investigations in hypothermia fatalities","volume":"127","author":"Palmiere","year":"2013","journal-title":"Int. J. Legal Med."},{"key":"2021111619313990700_B5","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1007\/s12024-010-9142-4","article-title":"Hypothermia","volume":"6","author":"Turk","year":"2010","journal-title":"Forensic Sci. Med. Pathol."},{"key":"2021111619313990700_B6","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1007\/s12024-009-9073-0","article-title":"Tsokos, M. (ed.): Forensic Pathology Reviews, Vol 5","volume":"5","author":"Seidl","year":"2009","journal-title":"Forensic Sci. Med. Pathol."},{"key":"2021111619313990700_B7","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1080\/20961790.2017.1330804","article-title":"Microscopic examination of pituitary glands in cases of fatal accidental hypothermia","volume":"2","author":"Doberentz","year":"2017","journal-title":"Forensic Sci. Res."},{"key":"2021111619313990700_B8","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.forsciint.2013.10.028","article-title":"Free fatty acids as markers of death from hypothermia","volume":"234","author":"Ba\u0144ka","year":"2014","journal-title":"Forensic Sci. Int."},{"key":"2021111619313990700_B9","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1007\/s00414-014-1138-2","article-title":"Victims of lethal hypothermia have decreased levels of thrombomodulin in myocardium and urine","volume":"129","author":"Pakanen","year":"2015","journal-title":"Int. J. Legal Med."},{"key":"2021111619313990700_B10","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1016\/j.legalmed.2015.09.005","article-title":"An objective approach using three indexes for determining fatal hypothermia due to cold exposure; statistical analysis of oxyhemoglobin saturation data","volume":"17","author":"Yajima","year":"2015","journal-title":"Leg. Med."},{"key":"2021111619313990700_B11","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.forsciint.2012.12.007","article-title":"Biochemical markers of fatal hypothermia","volume":"226","author":"Palmiere","year":"2013","journal-title":"Forensic Sci. Int."},{"key":"2021111619313990700_B12","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.forsciint.2013.03.003","article-title":"Glucocorticosteroids as markers of death from hypothermia","volume":"229","author":"Ba\u0144ka","year":"2013","journal-title":"Forensic Sci. Int."},{"key":"2021111619313990700_B13","doi-asserted-by":"publisher","first-page":"504","DOI":"10.3109\/10253890.2014.953477","article-title":"Changes in cardiac thrombomodulin and heat shock transcription factor 1 expression and peripheral thrombomodulin and catecholamines during hypothermia in rats","volume":"17","author":"Kaija","year":"2014","journal-title":"Stress"},{"key":"2021111619313990700_B14","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.jflm.2017.07.002","article-title":"Endocan concentrations in postmortem serum, vitreous humor and urine in victims of lethal hypothermia","volume":"50","author":"Descloux","year":"2017","journal-title":"J. Forensic Legal Med."},{"key":"2021111619313990700_B15","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1007\/s12024-017-9884-3","article-title":"Expression of Hsp27 and Hsp70 and vacuolization in the pituitary glands in cases of fatal hypothermia","volume":"13","author":"Doberentz","year":"2017","journal-title":"Forensic Sci. Med. Pathol."},{"key":"2021111619313990700_B16","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1007\/s12024-016-9764-2","article-title":"Catecholamines and their O -methylated metabolites in vitreous humor in hypothermia cases","volume":"12","author":"Hervet","year":"2016","journal-title":"Forensic Sci. Med. Pathol."},{"key":"2021111619313990700_B17","doi-asserted-by":"publisher","first-page":"1393","DOI":"10.1126\/science.aaf7537","article-title":"The TRPM2 channel is a hypothalamic heat sensor that limits fever and can drive hypothermia.","volume":"353","author":"Song","year":"2016","journal-title":"Science"},{"key":"2021111619313990700_B18","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/j.legalmed.2010.01.004","article-title":"Immunohistochemistry of catecholamines in the hypothalamic\u2013pituitary\u2013adrenal system with special regard to fatal hypothermia and hyperthermia","volume":"12","author":"Ishikawa","year":"2010","journal-title":"Leg. Med."},{"key":"2021111619313990700_B19","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1007\/s00414-009-0374-3","article-title":"Postmortem biochemistry and immunohistochemistry of chromogranin A as a stress marker with special regard to fatal hypothermia and hyperthermia","volume":"125","author":"Yoshida","year":"2011","journal-title":"Int. J. Legal Med."},{"key":"2021111619313990700_B20","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1021\/ar900274f","article-title":"New advances in the application of FTIR microscopy and spectroscopy for the characterization of artistic materials.","volume":"43","author":"Prati","year":"2010","journal-title":"Acc. Chem. Res."},{"key":"2021111619313990700_B21","doi-asserted-by":"publisher","first-page":"1444","DOI":"10.1021\/acs.analchem.7b05330","article-title":"Infrared spectroscopic imaging advances as an analytical technology for biomedical sciences","volume":"90","author":"Wrobel","year":"2017","journal-title":"Anal. Chem."},{"key":"2021111619313990700_B22","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1016\/j.tibtech.2010.07.003","article-title":"Clinical application of FTIR imaging: new reasons for hope","volume":"28","author":"Petibois","year":"2010","journal-title":"Trends Biotechnol."},{"key":"2021111619313990700_B23","doi-asserted-by":"publisher","first-page":"1803","DOI":"10.1039\/C5CS00585J","article-title":"Developing and understanding biofluid vibrational spectroscopy: a critical review","volume":"45","author":"Baker","year":"2015","journal-title":"Chem. Soc. Rev."},{"key":"2021111619313990700_B24","doi-asserted-by":"publisher","first-page":"1381","DOI":"10.1039\/C6AN01916A","article-title":"High definition infrared chemical imaging of colorectal tissue using a Spero QCL microscope.","volume":"142","author":"Bird","year":"2017","journal-title":"Analyst"},{"key":"2021111619313990700_B25","doi-asserted-by":"publisher","first-page":"3842","DOI":"10.1021\/acs.analchem.7b04505","article-title":"Au\/ZnSe-Based Surface Enhanced Infrared Absorption Spectroscopy as a Universal Platform for Bioanalysis","volume":"90","author":"Bao","year":"2018","journal-title":"Anal. Chem."},{"key":"2021111619313990700_B26","doi-asserted-by":"publisher","first-page":"3140","DOI":"10.1021\/acs.analchem.7b04318","article-title":"Multispectral atomic force microscopy-infrared nano-imaging of malaria infected red blood cells","volume":"90","author":"Perez-Guaita","year":"2018","journal-title":"Anal. Chem."},{"key":"2021111619313990700_B27","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1039\/C5CS00440C","article-title":"Spectral pre and post processing for infrared and Raman spectroscopy of biological tissues and cells.","volume":"45","author":"Byrne","year":"2016","journal-title":"Chem. Soc. Rev."},{"key":"2021111619313990700_B28","doi-asserted-by":"publisher","first-page":"1771","DOI":"10.1039\/C5CS00440C","article-title":"Using Fourier transform IR spectroscopy to analyze biological materials","volume":"9","author":"Baker","year":"2014","journal-title":"Nat. Protoc."},{"key":"2021111619313990700_B29","doi-asserted-by":"publisher","first-page":"1029","DOI":"10.1039\/b822130h","article-title":"Vibrational spectroscopy: a clinical tool for cancer diagnostics","volume":"134","author":"Kendall","year":"2009","journal-title":"Analyst"},{"key":"2021111619313990700_B30","doi-asserted-by":"publisher","first-page":"1208","DOI":"10.1039\/b820998g","article-title":"Differential diagnosis of cutaneous carcinomas by infrared spectral micro-imaging combined with pattern recognition","volume":"134","author":"Ly","year":"2009","journal-title":"Analyst"},{"key":"2021111619313990700_B31","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1039\/C5AN01512J","article-title":"Characterization of human breast cancer tissues by infrared imaging","volume":"141","author":"Verdonck","year":"2016","journal-title":"Analyst"},{"key":"2021111619313990700_B32","doi-asserted-by":"publisher","DOI":"10.1039\/C7AN01883E","article-title":"FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice","author":"Chrabaszcz","year":"2018","journal-title":"Analyst"},{"key":"2021111619313990700_B33","doi-asserted-by":"publisher","first-page":"179","DOI":"10.4137\/BMI.S592","article-title":"FTIR Microspectroscopy Coupled with Two-Class Discrimination Segregates Markers Responsible for Inter- and Intra-Category Variance in Exfoliative Cervical Cytology.","volume":"3","author":"Walsh","year":"2008","journal-title":"Biomark Insights"},{"key":"2021111619313990700_B34","doi-asserted-by":"publisher","first-page":"4005","DOI":"10.1039\/C3AN01022H","article-title":"Infrared spectral histopathology for cancer diagnosis: a novel approach for automated pattern recognition of colon adenocarcinoma","volume":"139","author":"Nallala","year":"2014","journal-title":"Analyst"},{"key":"2021111619313990700_B35","doi-asserted-by":"crossref","DOI":"10.1002\/jbio.201700260","article-title":"Rapid intra-operative diagnosis of kidney cancer by ATR-IR spectroscopy of tissue smears.","author":"Pucetaite","year":"2018","journal-title":"J. Biophotonics"},{"key":"2021111619313990700_B36","doi-asserted-by":"publisher","first-page":"8782","DOI":"10.1021\/acs.analchem.7b01025","article-title":"Using Infrared Spectroscopy and Multivariate Analysis to Detect Antibiotics\u2019 Resistant Escherichia coli Bacteria","volume":"89","author":"Sharaha","year":"2017","journal-title":"Anal. Chem."},{"key":"2021111619313990700_B37","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1016\/j.saa.2018.02.004","article-title":"Human papillomavirus detection using PCR and ATR-FTIR for cervical cancer screening","volume":"196","author":"Rymsza","year":"2018","journal-title":"Spectrochim. Acta, Part A"},{"key":"2021111619313990700_B38","doi-asserted-by":"publisher","first-page":"4769","DOI":"10.1039\/C4AN00989D","article-title":"Diagnosing malaria infected cells at the single cell level using focal plane array Fourier transform infrared imaging spectroscopy.","volume":"139","author":"Wood","year":"2014","journal-title":"Analyst"},{"key":"2021111619313990700_B39","doi-asserted-by":"publisher","first-page":"e201700256","DOI":"10.1002\/jbio.201700256","article-title":"Tendinopathy diagnosis and treatment monitoring using attenuated total reflectance-Fourier transform infrared spectroscopy","volume":"11","author":"Bhattacharjee","year":"2018","journal-title":"J. Biophotonics"},{"key":"2021111619313990700_B40","doi-asserted-by":"publisher","first-page":"1583","DOI":"10.1039\/C7AN01909B","article-title":"FTIR spectroscopy of chronic venous leg ulcer exudates: an approach to spectral healing marker identification","volume":"143","author":"Cerusico","year":"2018","journal-title":"Analyst"},{"key":"2021111619313990700_B41","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1039\/C7AN01747B","article-title":"Detection of A\u03b2 plaque-associated astrogliosis in Alzheimer\u2019s disease brain by spectroscopic imaging and immunohistochemistry","volume":"143","author":"Palombo","year":"2018","journal-title":"Analyst"},{"key":"2021111619313990700_B42","doi-asserted-by":"publisher","first-page":"4107","DOI":"10.1021\/acs.biochem.7b00262","article-title":"A Multimodal Spectroscopic Imaging Method To Characterize the Metal and Macromolecular Content of Proteinaceous Aggregates (\u201cAmyloid Plaques\u201d)","volume":"56","author":"Summers","year":"2017","journal-title":"Biochemistry"},{"key":"2021111619313990700_B43","doi-asserted-by":"publisher","first-page":"3991","DOI":"10.1039\/c3an00295k","article-title":"Synchrotron FTIR reveals lipid around and within amyloid plaques in transgenic mice and Alzheimer\u2019s disease brain","volume":"138","author":"Liao","year":"2013","journal-title":"Analyst"},{"key":"2021111619313990700_B44","doi-asserted-by":"publisher","first-page":"2711","DOI":"10.1021\/ac1002728","article-title":"Fourier transform infrared imaging showing reduced unsaturated lipid content in the hippocampus of a mouse model of Alzheimer\u2019s disease","volume":"82","author":"Leskovjan","year":"2010","journal-title":"Anal. Chem."},{"key":"2021111619313990700_B45","doi-asserted-by":"publisher","first-page":"17625","DOI":"10.1038\/srep17625","article-title":"Synchrotron FTIR micro-spectroscopy for structural analysis of Lewy bodies in the brain of Parkinson\u2019s disease patients","volume":"5","author":"Araki","year":"2015","journal-title":"Sci. Rep."},{"key":"2021111619313990700_B46","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/j.vibspec.2010.01.019","article-title":"Neural network algorithm for the early detection of Parkinson\u2019s disease from blood plasma by FTIR micro-spectroscopy","volume":"53","author":"Ahmed","year":"2010","journal-title":"Vib. Spectrosc."},{"key":"2021111619313990700_B47","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.abb.2006.12.027","article-title":"Biomolecular investigation of human substantia nigra in Parkinson\u2019s disease by synchrotron radiation Fourier transform infrared micro spectroscopy","volume":"459","author":"Szczerbowska-Boruchowska","year":"2007","journal-title":"Arch. Biochem. Biophys."},{"key":"2021111619313990700_B48","doi-asserted-by":"publisher","first-page":"1180","DOI":"10.1016\/j.neuroimage.2009.09.053","article-title":"Early detection of the chemical changes occurring during the induction and prevention of autoimmune-mediated demyelination detected by FT-IR imaging","volume":"49","author":"Heraud","year":"2010","journal-title":"NeuroImage"},{"key":"2021111619313990700_B49","doi-asserted-by":"publisher","first-page":"2339","DOI":"10.1016\/j.bbamem.2013.01.014","article-title":"FTIR spectroscopic imaging of protein aggregation in living cells","volume":"1828","author":"Miller","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"2021111619313990700_B50","doi-asserted-by":"publisher","first-page":"e1500911","DOI":"10.1126\/sciadv.1500911","article-title":"Mechanisms of murine cerebral malaria: Multimodal imaging of altered cerebral metabolism and protein oxidation at hemorrhage sites","volume":"1","author":"Hackett","year":"2015","journal-title":"Sci. Adv."},{"key":"2021111619313990700_B51","doi-asserted-by":"publisher","first-page":"10949","DOI":"10.1021\/acs.analchem.6b02588","article-title":"Concurrent Glycogen and Lactate Imaging with FTIR Spectroscopy to Spatially Localize Metabolic Parameters of the Glial Response following Brain Ischemia","volume":"88","author":"Hackett","year":"2016","journal-title":"Anal. Chem."},{"key":"2021111619313990700_B52","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.nbd.2016.03.006","article-title":"A novel multi-modal platform to image molecular and elemental alterations in ischemic stroke","volume":"91","author":"Caine","year":"2016","journal-title":"Neurobiol. Dis."},{"key":"2021111619313990700_B53","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1021\/cn500157j","article-title":"In Situ Biospectroscopic Investigation of Rapid Ischemic and Postmortem Induced Biochemical Alterations in the Rat Brain","volume":"6","author":"Hackett","year":"2015","journal-title":"ACS Chem. Neurosci."},{"key":"2021111619313990700_B54","doi-asserted-by":"publisher","first-page":"806","DOI":"10.1016\/j.freeradbiomed.2015.08.029","article-title":"Novel bio-spectroscopic imaging reveals disturbed protein homeostasis and thiol redox with protein aggregation prior to hippocampal CA1 pyramidal neuron death induced by global brain ischemia in the rat","volume":"89","author":"Hackett","year":"2015","journal-title":"Free Radical Biol. Med."},{"key":"2021111619313990700_B55","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1021\/acschemneuro.5b00037","article-title":"A new method to image heme-fe, total fe, and aggregated protein levels after intracerebral hemorrhage","volume":"6","author":"Hackett","year":"2015","journal-title":"ACS Chem. Neurosci."},{"key":"2021111619313990700_B56","doi-asserted-by":"publisher","first-page":"3088","DOI":"10.1016\/j.bbamem.2014.08.025","article-title":"Epileptic seizures induce structural and functional alterations on brain tissue membranes","volume":"1838","author":"Turker","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"2021111619313990700_B57","doi-asserted-by":"publisher","first-page":"111409","DOI":"10.1117\/1.JBO.18.11.111409","article-title":"Epileptic seizure-induced structural and functional changes in rat femur and tibia bone tissues: a Fourier transform infrared imaging study","volume":"18","author":"Garip","year":"2013","journal-title":"J. Biomed. Opt."},{"key":"2021111619313990700_B58","volume-title":"Seeley\u2019s Anatomy & Physiology","author":"Seeley","year":"2014","edition":"10th edn"},{"key":"2021111619313990700_B59","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.jflm.2013.10.014","article-title":"Lethal hypothermia in an animal model, not associated with basal renal epithelial vacuolization","volume":"21","author":"Zhou","year":"2014","journal-title":"J. Forensic & Legal Med."},{"key":"2021111619313990700_B60","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1080\/00450618.2016.1156157","article-title":"Preliminary study on fatal hyperthermia in rat liver tissue by Fourier transform infrared microspectroscopy","volume":"49","author":"Wang","year":"2017","journal-title":"Aust. J. Forensic Sci."},{"key":"2021111619313990700_B61","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.chemolab.2012.03.004","article-title":"Extended multiplicative signal correction in vibrational spectroscopy, a tutorial","volume":"117","author":"Afseth","year":"2012","journal-title":"Chemometrics Intell. Lab. Syst."},{"key":"2021111619313990700_B62","doi-asserted-by":"publisher","first-page":"2812","DOI":"10.1039\/C3AY41907J","article-title":"Principal component analysis","volume":"6","author":"Smilde","year":"2014","journal-title":"Anal. Methods"},{"key":"2021111619313990700_B63","doi-asserted-by":"publisher","first-page":"3668","DOI":"10.1039\/C5AN02452H","article-title":"Combining random forest and 2D correlation analysis to identify serum spectral signatures for neuro-oncology","volume":"141","author":"Smith","year":"2016","journal-title":"Analyst"},{"key":"2021111619313990700_B64","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-642-20659-7","volume-title":"Forensic Histopathology: Fundamentals and Perspectives","author":"Dettmeyer","year":"2011","edition":"1st edn"},{"key":"2021111619313990700_B65","doi-asserted-by":"publisher","first-page":"3624","DOI":"10.1016\/j.neuroimage.2011.11.033","article-title":"The application of Fourier transform infrared microspectroscopy for the study of diseased central nervous system tissue","volume":"59","author":"Caine","year":"2012","journal-title":"NeuroImage"},{"key":"2021111619313990700_B66","doi-asserted-by":"publisher","first-page":"1961","DOI":"10.1007\/s00216-006-0828-0","article-title":"Analysis of type I and IV collagens by FT-IR spectroscopy and imaging for a molecular investigation of skeletal muscle connective tissue","volume":"386","author":"Petibois","year":"2006","journal-title":"Anal. Bioanal. Chem."},{"key":"2021111619313990700_B67","doi-asserted-by":"publisher","first-page":"e201700044","DOI":"10.1002\/jbio.201700044","article-title":"A possible Fourier transform infrared-based plasma fingerprint of angiotensin-converting enzyme inhibitor-induced reversal of endothelial dysfunction in diabetic mice","volume":"11","author":"Staniszewska-Slezak","year":"2018","journal-title":"J. Biophotonics"},{"key":"2021111619313990700_B68","first-page":"1795","article-title":"Optical analysis of glioma: Fourier-transform infrared spectroscopy reveals the IDH1 mutation status","author":"Uckermann","year":"2017","journal-title":"Clin. Cancer Res."},{"key":"2021111619313990700_B69","doi-asserted-by":"publisher","first-page":"1170","DOI":"10.1111\/php.12110","article-title":"Morphological and structural changes in lung tissue infected by paracoccidioides brasiliensis: FTIR photoacoustic spectroscopy and histological analysis","volume":"89","author":"Morato","year":"2013","journal-title":"Photochem. Photobiol."},{"key":"2021111619313990700_B70","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2105-10-213","article-title":"A comparison of random forest and its Gini importance with standard chemometric methods for the feature selection and classification of spectral data","volume":"10","author":"Menze","year":"2009","journal-title":"BMC Bioinf."},{"key":"2021111619313990700_B71","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1177\/153303460400300613","article-title":"Characteristic absorbance of nucleic acids in the Mid-IR region as possible common biomarkers for diagnosis of malignancy","volume":"3","author":"Sahu","year":"2004","journal-title":"Technol. Cancer Res. Treat."},{"key":"2021111619313990700_B72","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1038\/s41598-018-19303-3","article-title":"Relapsing-Remitting Multiple Sclerosis diagnosis from cerebrospinal fluids via Fourier transform infrared spectroscopy coupled with multivariate analysis","volume":"8","author":"Yonar","year":"2018","journal-title":"Sci. Rep."},{"key":"2021111619313990700_B73","doi-asserted-by":"publisher","first-page":"323","DOI":"10.3171\/jns.1980.53.3.0323","article-title":"Effect of transient ischemia on free fatty acids and phospholipids in the gerbil brain. Lipid peroxidation as a possible cause of postischemic injury","volume":"53","author":"Yoshida","year":"1980","journal-title":"J. Neurosurg."},{"key":"2021111619313990700_B74","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.abb.2012.02.012","article-title":"Amifostine, a radioprotectant agent, protects rat brain tissue lipids against ionizing radiation induced damage: an FTIR microspectroscopic imaging study","volume":"520","author":"Cakmak","year":"2012","journal-title":"Arch. Biochem. Biophys."},{"key":"2021111619313990700_B75","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.vibspec.2011.10.016","article-title":"Tissue acquisition and storage associated oxidation considerations for FTIR microspectroscopic imaging of polyunsaturated fatty acids","volume":"60","author":"Stitt","year":"2012","journal-title":"Vib. Spectrosc."},{"key":"2021111619313990700_B76","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1093\/clinchem\/41.12.1819","article-title":"Lipid peroxidation and antioxidants as biomarkers of tissue damage","volume":"41","author":"Gutteridge","year":"1995","journal-title":"Clin. Chem."},{"key":"2021111619313990700_B77","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.chemphyslip.2016.06.002","article-title":"Effect of cis-(Z)-flupentixol on DPPC membranes in the presence and absence of cholesterol","volume":"198","author":"Yonar","year":"2016","journal-title":"Chem. Phys. Lipids"},{"key":"2021111619313990700_B78","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.bbamem.2004.12.009","article-title":"Melatonin strongly interacts with zwitterionic model membranes\u2014evidence from Fourier transform infrared spectroscopy and differential scanning calorimetry","volume":"1668","author":"Severcan","year":"2005","journal-title":"Biochim. Biophys. Acta"},{"key":"2021111619313990700_B79","doi-asserted-by":"publisher","first-page":"1624","DOI":"10.1039\/C8AN00017D","article-title":"Deciphering the biochemical similarities and differences among mouse embryonic stem cells, somatic and cancer cells using ATR-FTIR spectroscopy","volume":"143","author":"G\u00fcler","year":"2018","journal-title":"Analyst"},{"key":"2021111619313990700_B80","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1038\/nprot.2015.024","article-title":"Obtaining information about protein secondary structures in aqueous solution using Fourier transform IR spectroscopy","volume":"10","author":"Yang","year":"2015","journal-title":"Nat. Protoc."},{"key":"2021111619313990700_B81","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1080\/0265673031000119006","article-title":"Basic principles of thermal dosimetry and thermal thresholds for tissue damage from hyperthermia","volume":"19","author":"Dewhirst","year":"2003","journal-title":"Int. J. Hyperthermia"},{"key":"2021111619313990700_B82","doi-asserted-by":"publisher","first-page":"1493","DOI":"10.1007\/s00249-010-0607-x","article-title":"Effects of hypericin on the structure and aggregation properties of \u03b2-amyloid peptides","volume":"39","author":"Bramanti","year":"2010","journal-title":"Eur. Biophys. J."},{"key":"2021111619313990700_B83","doi-asserted-by":"publisher","first-page":"1020","DOI":"10.1016\/j.bpj.2012.07.046","article-title":"The \u03b1 -Helix to \u03b2 -Sheet Transition in Stretched and Compressed Hydrated Fibrin Clots","volume":"103","author":"Litvinov","year":"2012","journal-title":"Biophys. J."},{"key":"2021111619313990700_B84","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1056\/NEJM199702203360803","article-title":"Treatment of Traumatic Brain Injury with Moderate Hypothermia","volume":"336","author":"Jennett","year":"1997","journal-title":"N. Engl. J. Med."},{"key":"2021111619313990700_B85","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1016\/j.mehy.2005.12.048","article-title":"Pathophysiological factors underlying heatstroke","volume":"67","author":"Yan","year":"2006","journal-title":"Med. Hypotheses"},{"key":"2021111619313990700_B86","doi-asserted-by":"publisher","first-page":"1725","DOI":"10.1152\/japplphysiol.01143.2001","article-title":"Invited review: Effects of heat and cold stress on mammalian gene expression","volume":"92","author":"Sonna","year":"2002","journal-title":"J. Appl. Physiol."},{"key":"2021111619313990700_B87","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/978-4-431-66882-4_3","article-title":"Therapeutic hypothermia in experimental models of traumatic brain injury In: Hayashi","volume-title":"Brain Hypothermia: Pathology, Pharmacology, and Treatment of Severe Brain Injury","author":"Dietrich","year":"2000"},{"key":"2021111619313990700_B88","doi-asserted-by":"publisher","first-page":"239","DOI":"10.4323\/rjlm.2010.239","article-title":"Morphological diagnosis of hyperthermia-related deaths","volume":"18","author":"Ceausu","year":"2010","journal-title":"Romanian J. Legal Med."},{"key":"2021111619313990700_B89","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1007\/s00414-017-1721-4","article-title":"Identification of pulmonary edema in forensic autopsy cases of fatal anaphylactic shock using Fourier transform infrared microspectroscopy","volume":"132","author":"Lin","year":"2018","journal-title":"Int. J. Legal Med."},{"key":"2021111619313990700_B90","doi-asserted-by":"publisher","first-page":"2708","DOI":"10.1021\/acs.analchem.7b04642","article-title":"Identification of pulmonary edema in forensic autopsy cases of sudden cardiac death using Fourier transform infrared microspectroscopy: a pilot study","volume":"90","author":"Lin","year":"2018","journal-title":"Anal. Chem."}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20181633\/841800\/bsr-2018-1633.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20181633\/841800\/bsr-2018-1633.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T15:22:35Z","timestamp":1637076155000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/39\/3\/BSR20181633\/111015\/Biochemical-detection-of-fatal-hypothermia-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3]]},"references-count":90,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3,29]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20181633","relation":{"is-referenced-by":[{"id-type":"doi","id":"10.1038\/s41598-025-00963-x","asserted-by":"object"}]},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,3]]},"published":{"date-parts":[[2019,3]]},"article-number":"BSR20181633"}}