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CD4+ naive T cells from the spleen and lymph nodes were collected after 24 h of co-culture with BECs. The number of Th2 and Th17 cells was elevated in the HDM and HDM+LPS groups compared with the control group; these responses were exacerbated when exposed to HDM+LPS. The number of HDM- and HDM+LPS-specific Th2\/Th17 cells in young mice was higher than old mice; however, the Th2:Th17 cell ratio was greater in young mice, whereas the Th17:Th2 cell ratio was greater in old mice. The expression of GATA-3 and RORc was increased in the HDM+LPS and HDM groups compared with the PBS group and exhibited most in HDM+LPS group. The expression of HDM+LPS-specific GATA-3 in young mice was higher, while the expression of HDM+LPS-specific RORc in old mice was higher. Murine BECs directly regulated CD4+ naive T-cell differentiation under allergen exposure.<\/jats:p>","DOI":"10.1042\/bsr20181948","type":"journal-article","created":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T17:15:17Z","timestamp":1544634917000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Bronchial epithelial cells of young and old mice directly regulate the differentiation of Th2 and Th17"],"prefix":"10.1042","volume":"39","author":[{"given":"Da","family":"Liu","sequence":"first","affiliation":[{"name":"The Second Xiangya Hospital of Central South University, Changsha, Hunan Province 410011, China"},{"name":"Department of Respiratory Medicine, Changsha central hospital, Changsha, Hunan province 410111, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Long","family":"He","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Puai Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, Wuhan, Hubei Province 430030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Emergency, Changsha Central Hospital, Changsha, Hunan Province 410004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjin","family":"Sun","sequence":"additional","affiliation":[{"name":"The Second Xiangya Hospital of Central South University, Changsha, Hunan Province 410011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lulu","family":"Qiu","sequence":"additional","affiliation":[{"name":"The Second Xiangya Hospital of Central South University, Changsha, Hunan Province 410011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Xu","sequence":"additional","affiliation":[{"name":"The Second Xiangya Hospital of Central South University, Changsha, Hunan Province 410011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aijun","family":"Jia","sequence":"additional","affiliation":[{"name":"The Second Xiangya Hospital of Central South University, Changsha, Hunan Province 410011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cong","family":"Peng","sequence":"additional","affiliation":[{"name":"The Second Xiangya Hospital of Central South University, Changsha, Hunan Province 410011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongshan","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Second Xiangya Hospital of Central South University, Changsha, Hunan Province 410011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4043-4009","authenticated-orcid":false,"given":"Xudong","family":"Xiang","sequence":"additional","affiliation":[{"name":"The Second Xiangya Hospital of Central South University, Changsha, Hunan Province 410011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2019,2,1]]},"reference":[{"key":"2021111619283228300_B1","volume-title":"Global Strategy for Asthma Management and Prevention. 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