{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T16:02:18Z","timestamp":1792684938837,"version":"4.5.0"},"reference-count":28,"publisher":"Portland Press Ltd.","issue":"2","license":[{"start":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:00:00Z","timestamp":1567987200000},"content-version":"vor","delay-in-days":220,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,2,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Soybean is the most important genetically modified (GM) oilseed worldwide. Regulations relating to the approval of biotech soybean varieties and product labeling demand accurate and reliable detection techniques to screen for GM soya. High-quality extracted DNA is essential for DNA-based monitoring methods. Thus, four widely used protocols (SDS, CTAB, DP305, and DNeasy Plant Mini Kit) were compared in the present study to explore the most efficient DNA extraction method for raw soya matrix. The SDS-based method showed the highest applicability. Then crucial factors influencing DNA yield and purity, such as SDS lysis buffer component concentrations and organic compounds used to isolate DNA, were further investigated to improve the DNA obtained from raw soybean seeds, which accounts for the innovation of this work. As a result, lysis buffer (2% SDS (w\/v), 150 mM NaCl, 50 mM Tris\/HCl, 50 mM EDTA, pH 8.0) and organic reagents including chloroform\/isoamyl alcohol (24:1, v\/v) (C: I), isopropanol, and ethanol corresponding to the extraction and first and second precipitation procedures, respectively, were used in the optimized SDS method. The optimized method was verified by extracting approximately 2020\u20132444 ng DNA\/mg soybean with A260\/280 ratios of 1.862\u20131.954 from five biotech and non-biotech soybean varieties. Only 0.5 mg of soya was required to obtain enough DNA for PCR amplification using the optimized SDS-based method. These results indicate that the screening protocol in the present study achieves the highest suitability and efficiency for DNA isolation from raw soya seed flour.<\/jats:p>","DOI":"10.1042\/bsr20182271","type":"journal-article","created":{"date-parts":[[2019,1,15]],"date-time":"2019-01-15T16:57:27Z","timestamp":1547571447000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":96,"title":["A modified SDS-based DNA extraction method from raw soybean"],"prefix":"10.1042","volume":"39","author":[{"given":"Yimiao","family":"Xia","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fusheng","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Du","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanhao","family":"Bu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Xin","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boye","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2019,2,5]]},"reference":[{"key":"2021111619292760900_B1","article-title":"Oilseeds: World Markets and Trade","author":"Foreign Agricultural Service and United States Department of Agriculture","year":"2018"},{"key":"2021111619292760900_B2","article-title":"Global Status of Commercialized Biotech\/GM Crops in 2017: Biotech Crop Adoption Surges as Economic Benefits Accumulate in 22 Years","volume-title":"ISAAA Brief No. 53","author":"ISAAA","year":"2017"},{"key":"2021111619292760900_B3","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1016\/j.foodcont.2015.06.052","article-title":"Monitoring the prevalence of genetically modified (GM) soybean in Turkish food and feed products","volume":"59","author":"Turkec","year":"2016","journal-title":"Food Control"},{"key":"2021111619292760900_B4","first-page":"24","article-title":"Regulation (EC) No 1830\/2003 of the European Parliament","volume":"L268","author":"The Commission of the European Communities","year":"2003","journal-title":"Off. J. Eur. Union."},{"key":"2021111619292760900_B5","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1016\/j.foodres.2009.10.003","article-title":"Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques","volume":"43","author":"Costa","year":"2010","journal-title":"Food Res. Int."},{"key":"2021111619292760900_B6","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1016\/j.foodchem.2017.08.022","article-title":"DNA-based techniques for authentication of processed food and food supplements","volume":"240","author":"Lo","year":"2018","journal-title":"Food Chem."},{"key":"2021111619292760900_B7","doi-asserted-by":"publisher","first-page":"1183","DOI":"10.1016\/j.foodcont.2008.01.004","article-title":"Comparative study of DNA extraction methods for soybean derived food products","volume":"19","author":"Mafra","year":"2008","journal-title":"Food Control"},{"key":"2021111619292760900_B8","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.foodcont.2011.09.001","article-title":"Assessment of DNA extraction methods for PCR testing of discontinued or unapproved biotech events in single seeds of canola, flax and soybean","volume":"24","author":"Demekea","year":"2012","journal-title":"Food Control"},{"key":"2021111619292760900_B9","doi-asserted-by":"publisher","first-page":"3803","DOI":"10.5504\/BBEQ.2013.0026","article-title":"A modified CTAB method for DNA extraction from soybean and meat products","volume":"27","author":"Stefanova","year":"2013","journal-title":"Biotechnol. Biotec. Eq."},{"key":"2021111619292760900_B10","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1270\/jsbbs.53.277","article-title":"Rapid DNA extraction method from soybean seeds","volume":"53","author":"Kamiya","year":"2003","journal-title":"Breed Sci."},{"key":"2021111619292760900_B11","doi-asserted-by":"publisher","first-page":"1300","DOI":"10.1016\/j.foodcont.2006.08.009","article-title":"Variations of tissue DNA density and nuclear DNA content in soybean lines and their impacts on the GMO quantification","volume":"18","author":"Xu","year":"2007","journal-title":"Food Control"},{"key":"2021111619292760900_B12","volume-title":"Precipitation of DNA with Ethanol - A Laboratory Manual","author":"Green","year":"2016"},{"key":"2021111619292760900_B13","volume-title":"Isolation of High-Molecular-Weight DNA Using Organic Solvents - A Laboratory Manual","author":"Green","year":"2017"},{"key":"2021111619292760900_B14","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/BF02712670","article-title":"A plant DNA minipreparation: version II","volume":"1","author":"Dellaporta","year":"1983","journal-title":"Plant Mol. Biol. Rep."},{"key":"2021111619292760900_B15","article-title":"Foodstuffs-Methods of analysis for the detection of genetically modified organisms and derived products-nucleic acid extraction","author":"International Standard (ISO) 21571","year":"2005"},{"key":"2021111619292760900_B16","doi-asserted-by":"publisher","first-page":"4586","DOI":"10.1021\/jf300827q","article-title":"Comparison of three DNA extraction methods for feed products and four amplification methods for the 5\u2032-junction fragment of Roundup Ready soybean","volume":"60","author":"Wang","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"2021111619292760900_B17","doi-asserted-by":"publisher","first-page":"3686","DOI":"10.1007\/s12010-014-0760-2","article-title":"Influence of different processing treatments on the detectability of nucleic acid and protein targets in transgenic soybean meal","volume":"172","author":"Tian","year":"2014","journal-title":"Appl. Biochem. Biotech."},{"key":"2021111619292760900_B18","doi-asserted-by":"publisher","first-page":"1883","DOI":"10.1016\/j.foodchem.2010.12.013","article-title":"DNA extraction methods for detecting genetically modified foods: a comparative study","volume":"126","author":"Elsanhoty","year":"2011","journal-title":"Food Chem."},{"key":"2021111619292760900_B19","first-page":"123","article-title":"Three different extraction methods for detecting roundup ready soybean in processed food from the Italian market","volume":"3","author":"Milia","year":"2008","journal-title":"Int. J. Integr. Biol."},{"key":"2021111619292760900_B20","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/S0301-4622(02)00107-2","article-title":"Studies on surfactant-biopolymer interaction. I. Microcalorimetric investigation on the interaction of cetyltrimethylammonium bromide (CTAB) and sodium dodecylsulfate (SDS) with gelatin (Gn), lysozyme (Lz) and deoxyribonucleic acid (DNA)","volume":"98","author":"Chatterjee","year":"2002","journal-title":"Biophys. Chem."},{"key":"2021111619292760900_B21","first-page":"1","article-title":"A modified SDS-based DNA extraction method for high quality environmental DNA from seafloor environments","volume":"7","author":"Perumal","year":"2016","journal-title":"Front. Microbiol."},{"key":"2021111619292760900_B22","first-page":"1","article-title":"DNA extraction protocol for plants with high levels of secondary metabolites and polysaccharides without using liquid nitrogen and phenol","volume":"2012","author":"Sahu","year":"2012","journal-title":"Int. Math Scho. Notices"},{"key":"2021111619292760900_B23","doi-asserted-by":"publisher","first-page":"6160","DOI":"10.1021\/jf0704920","article-title":"Chemical composition of glyphosate-tolerant soybean 40-3-2 grown in europe remains equivalent with that of conventional soybean (Glycine max L","volume":"55","author":"Harrigan","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"2021111619292760900_B24","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1007\/BF02772108","article-title":"Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components","volume":"15","author":"Porebski","year":"1997","journal-title":"Plant Mol. Biol. Rep."},{"key":"2021111619292760900_B25","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1042\/bj0640405","article-title":"A new method for the isolation of ribonucleic acids from mammalian tissues","volume":"64","author":"Kirby","year":"1956","journal-title":"Biochem. J."},{"key":"2021111619292760900_B26","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/S0021-9258(18)74048-9","article-title":"The isolation of the components of streptoeoeeal nueleoproteins in serologieally active form","volume":"124","author":"Sevag","year":"1938","journal-title":"J. Biol. Chem."},{"key":"2021111619292760900_B27","doi-asserted-by":"publisher","first-page":"24","DOI":"10.3358\/shokueishi.42.24","article-title":"A multiplex PCR method of detecting recombinant DNAs from five lines of genetically modified maize","volume":"42","author":"Matsuoka","year":"2001","journal-title":"J. Food Hyg. Soc. Jpn."},{"key":"2021111619292760900_B28","doi-asserted-by":"publisher","first-page":"4632","DOI":"10.4238\/2012.September.14.1","article-title":"A protocol for high-quality genomic DNA extraction from legumes","volume":"11","author":"Agbagwa","year":"2012","journal-title":"Genet. Mol. Res."}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20182271\/844635\/bsr-2018-2271.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20182271\/844635\/bsr-2018-2271.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T20:21:18Z","timestamp":1637094078000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/39\/2\/BSR20182271\/110910\/A-modified-SDS-based-DNA-extraction-method-from"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2,28]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20182271","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,2]]},"published":{"date-parts":[[2019,2]]},"article-number":"BSR20182271"}}