{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T04:26:36Z","timestamp":1767155196064,"version":"build-2238731810"},"reference-count":30,"publisher":"Portland Press Ltd.","issue":"7","license":[{"start":{"date-parts":[[2019,9,19]],"date-time":"2019-09-19T00:00:00Z","timestamp":1568851200000},"content-version":"vor","delay-in-days":80,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Uterine leiomyoma is the most common benign smooth muscle tumor of uterus in women of reproductive age, with a high lifetime incidence. Nowadays, the exploration on the pharmacotherapies, such as progesterone receptor antagonist (PRA) requires more attention. Hence, the current study aimed to examine whether mifepristone, a PRA, influences the autophagy and apoptosis of uterine leiomyoma cells. Primary uterine leiomyoma cells were collected from 36 patients diagnosed with uterine leiomyoma to establish PR-M-positive (PR-M[+]) cells. The lentiviral vector overexpressing or silencing PR-M was subsequently delivered into one part of PR-M(+) cells in order to evaluate the role of PR-M in PR-M(+) cells. The results obtained revealed that cell viability was increased, while cell autophagy and apoptosis were diminished in the PR-M(+) cells treated with overexpressed PR-M, whereby the Bcl-2 level was elevated and the level of Beclin1 was reduced. An opposite trends were identified following treatment with knockdown of PR-M. Mifepristone at different concentrations (low, moderate, or high) was then applied to treat another part of the PR-M(+) cells. Mifepristone was identified to promote cell autophagy and apoptosis, decrease Bcl-2 level and increase Beclin1 level, accompanied by weakened interaction between Bcl-2 and Beclin1. Moreover, these effects of mifepristone on PR-M(+) cells were enhanced with increasing of the concentration. Taken together, the present study present evidence indicates the ability of PRA to regulate the Bcl-2\/Beclin1 axis, ultimately promoting the autophagy and apoptosis of uterine leiomyoma cells, highlighting that PRA serves as a promising therapeutic target for the treatment of uterine leiomyoma.<\/jats:p>","DOI":"10.1042\/bsr20190094","type":"journal-article","created":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T14:25:12Z","timestamp":1561991112000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Progesterone receptor antagonist provides palliative effects for uterine leiomyoma through a Bcl-2\/Beclin1-dependent mechanism"],"prefix":"10.1042","volume":"39","author":[{"given":"Lindong","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quanling","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiting","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pingping","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9618-9375","authenticated-orcid":false,"given":"Shihong","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2019,7,23]]},"reference":[{"key":"2021111619444912200_B1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.bpobgyn.2015.11.018","article-title":"The epidemiology and genetics of uterine leiomyoma","volume":"34","author":"Styer","year":"2016","journal-title":"Best Pract. Res. Clin. Obstet. Gynaecol."},{"key":"2021111619444912200_B2","doi-asserted-by":"publisher","first-page":"16043","DOI":"10.1038\/nrdp.2016.43","article-title":"Uterine fibroids","volume":"2","author":"Stewart","year":"2016","journal-title":"Nat. Rev. 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