{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T02:41:22Z","timestamp":1790044882256,"version":"4.0.1"},"reference-count":39,"publisher":"Portland Press Ltd.","issue":"12","license":[{"start":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T00:00:00Z","timestamp":1575244800000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,12,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Stromal cell derived factor-1 (SDF-1) and basic fibroblast growth factor (bFGF) were reported to induce the differentiation of bone marrow stem cells (BMSCs) into cells with characteristics of periodontal ligament fibroblasts. Thus SDF-1 and bFGF may play a positive role in BMSCs-mediated periodontal ligament regeneration. Here, the methylthiazolyldiphenyl tetrazolium bromide (MTT) assay was used to investigate the effect of scaffolds, SDF-1 and bFGF on BMSCs proliferation. RT-PCR and Western blot were used to evaluate gene and protein expression. Beagle dogs were used to establish an animal model of tooth reimplantation and to investigate the effects of scaffolds, BMSCs, SDF-1 and bFGF on periodontal ligament regeneration. X-ray images and micro computed tomography (micro CT) were used to assess morphological changes in replanted teeth and surrounding alveolar bone. H&amp;E staining and Masson\u2019s staining were also performed. BMSCs from Beagle dogs growth on scaffolds consisted of dense structured collagens. SDF-1 and bFGF effectively promoted the differentiation of BMSCs into fibroblasts, periodontal membrane reconstruction, and cell proliferation in vitro. SDF-1 and bFGF also stimulated the expression of type I collagen (Col I), type III collagen (Col III), CXC family chemokine receptor 4 (CXCR4), and S100 calcium binding protein A4 (S100A4), and decreased the expression of alkaline phosphatase (ALP). In our experimental Beagle dog model of tooth extraction and replantation, application of SDF-1 and bFGF significantly elevated periodontal membrane reconstruction and thus supported the survival of replanted teeth. In conclusion, the findings from the present study demonstrated that SDF-1 and bFGF enhance the process of periodontal ligament reconstruction, and provide a basis and reference for the use of stem cell tissue engineering in promoting periodontal membrane regeneration.<\/jats:p>","DOI":"10.1042\/bsr20190785","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T19:29:43Z","timestamp":1575314983000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Combination of SDF-1 and bFGF promotes bone marrow stem cell-mediated periodontal ligament regeneration"],"prefix":"10.1042","volume":"39","author":[{"given":"Mengting","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, Stomatological Center, Shenzhen People\u2019s Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, Guangdong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2053-1074","authenticated-orcid":false,"given":"Li","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Sichuan Academy of Medical Sciences and Sichuan Provincial People\u2019s Hospital, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2019,12,17]]},"reference":[{"key":"2021111619561733600_B1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2018\/5450768","article-title":"Detection, Characterization, and Clinical Application of Mesenchymal Stem Cells in Periodontal Ligament Tissue","author":"Tomokiyo","year":"2018","journal-title":"Stem Cells Int."},{"key":"2021111619561733600_B2","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/jre.12596","article-title":"Developmental pathways of periodontal tissue regeneration: developmental diversities of tooth morphogenesis do also map capacity of periodontal tissue regeneration?","volume":"54","author":"Ripamonti","year":"2019","journal-title":"J. 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