{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:09:29Z","timestamp":1787004569605,"version":"build-2736575974"},"reference-count":43,"publisher":"Portland Press Ltd.","issue":"9","license":[{"start":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T00:00:00Z","timestamp":1569542400000},"content-version":"vor","delay-in-days":26,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Background: The liver-specific microRNA-122 (miR-122) has been demonstrated as a powerful and promising biomarker of hepatic diseases. However, the researches on the accuracy of miR122 detection in chronic viral hepatitis have been inconsistent, leading us to conduct this meta-analysis to systematically summarize the diagnostic value of circulating miR-122 in patients with hepatitis B virus (HBV) and\/or hepatitis C virus (HCV)-associated chronic viral hepatitis.<\/jats:p>\n                  <jats:p>Methods: A comprehensive literature search (updated to January 30, 2019) in PubMed, Cochrane library, EMBASE, CNKI, Wanfang, and CQVIP databases was performed to identify eligible studies. The sensitivity (SEN), specificity (SPE), positive and negative likelihood ratios (PLR and NLR), diagnostic odds ratio (DOR), and area under the curve (AUC) were pooled to explore the diagnostic performance of circulating miR-122. Subgroup and threshold effect analysis were further carried out to explore the heterogeneity.<\/jats:p>\n                  <jats:p>Results: Overall, 15 studies were finally included in this meta-analysis according to the exclusion and inclusion criteria. The pooled estimates indicated a moderately high diagnostic accuracy for circulating miR-122, with a sensitivity of 0.92 [95% confidence interval (CI), 0.86\u20130.95], a specificity of 0.84 (95% CI, 0.78\u20130.89), a PLR of 5.7 (95% CI, 4.7\u20138.1), a NLR of 0.1 (95% CI, 0.06\u20130.18), a DOR of 57 (95% CI 25-129), and an AUC of 0.93 (95% CI, 0.91\u20130.95). The subgroup analysis demonstrated that diagnostic accuracy was better for HCV-associated chronic viral hepatitis patients and non-Chinese compared with other subgroups. In addition, we found that serum might be a more promising matrix for detecting the expression of miR-122 than plasma.<\/jats:p>\n                  <jats:p>Conclusions: Our results demonstrated that circulating miR-122 have a relatively high diagnostic value for chronic viral hepatitis detection, especially in the patients with HCV-associated chronic viral hepatitis. However, further large cohort studies are still required to confirm our findings.<\/jats:p>","DOI":"10.1042\/bsr20190900","type":"journal-article","created":{"date-parts":[[2019,8,19]],"date-time":"2019-08-19T12:15:19Z","timestamp":1566216919000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Diagnostic value of circulating miRNA-122 for hepatitis B virus and\/or hepatitis C virus-associated chronic viral hepatitis"],"prefix":"10.1042","volume":"39","author":[{"given":"Xinhao","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"},{"name":"School of Laboratory Medicine, Hubei University of Chinese Medicine, Huangjia Lake West Road, Wuhan 430065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqiang","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiulong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4147-2528","authenticated-orcid":false,"given":"Changqing","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2019,9,6]]},"reference":[{"key":"2021111619494204900_B1","doi-asserted-by":"publisher","first-page":"S35","DOI":"10.1002\/hep.22882","article-title":"Recommendations for identification and public health management of persons with chronic hepatitis B virus infection","volume":"49","author":"Weinbaum","year":"2009","journal-title":"Hepatology"},{"key":"2021111619494204900_B2","volume-title":"Screening for Hepatitis B Virus Infection in Nonpregnant Adolescents and Adults: Systematic Review to Update the 2004 U.S. Preventive Services Task Force Recommendation","author":"Chou","year":"2014"},{"key":"2021111619494204900_B3","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.1016\/j.jhep.2013.12.026","article-title":"Biomarkers of liver cell death","volume":"60","author":"Eguchi","year":"2014","journal-title":"J. Hepatol."},{"key":"2021111619494204900_B4","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1016\/j.clinre.2015.12.010","article-title":"Hepatocellular carcinoma diagnosis: circulating microRNAs emerge as robust biomarkers","volume":"40","author":"Gougelet","year":"2016","journal-title":"Clin. Res. Hepatol. Gastroenterol."},{"key":"2021111619494204900_B5","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1080\/1354750X.2018.1528631","article-title":"Circulating liver-specific microRNAs as noninvasive diagnostic biomarkers of hepatic diseases in human","volume":"24","author":"Musaddaq","year":"2018","journal-title":"Biomarker"},{"key":"2021111619494204900_B6","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1038\/nrc1840","article-title":"Oncomirs \u2013 microRNAs with a role in cancer","volume":"6","author":"Esquela-Kerscher","year":"2006","journal-title":"Nat. Rev. Cancer"},{"key":"2021111619494204900_B7","doi-asserted-by":"publisher","first-page":"e3694","DOI":"10.1371\/journal.pone.0003694","article-title":"Detection of microRNA expression in human peripheral blood microvesicles","volume":"3","author":"Hunter","year":"2008","journal-title":"PLoS One"},{"key":"2021111619494204900_B8","doi-asserted-by":"publisher","first-page":"10513","DOI":"10.1073\/pnas.0804549105","article-title":"Circulating microRNAs as stable blood-based markers for cancer detection","volume":"105","author":"Mitchell","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021111619494204900_B9","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1586\/erm.10.11","article-title":"Micromarkers: miRNAs in cancer diagnosis and prognosis","volume":"10","author":"Ferracin","year":"2010","journal-title":"Expert Rev. Mol. Diagn."},{"key":"2021111619494204900_B10","doi-asserted-by":"publisher","first-page":"5003","DOI":"10.1073\/pnas.1019055108","article-title":"Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma","volume":"108","author":"Arroyo","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021111619494204900_B11","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1111\/jvh.12075","article-title":"Serum microRNA-122 kinetics in patients with chronic hepatitis C virus infection during antiviral therapy","volume":"20","author":"Koberle","year":"2013","journal-title":"J. Viral Hepat."},{"key":"2021111619494204900_B12","doi-asserted-by":"publisher","first-page":"1507","DOI":"10.1002\/jmv.23987","article-title":"Micro-RNA-122 levels in acute liver failure and chronic hepatitis C","volume":"86","author":"Dubin","year":"2014","journal-title":"J. Med. Virol."},{"key":"2021111619494204900_B13","doi-asserted-by":"publisher","first-page":"e92112","DOI":"10.1371\/journal.pone.0092112","article-title":"Comparison of circulating, hepatocyte specific messenger RNA and microRNA as biomarkers for chronic hepatitis B and C","volume":"9","author":"Zhang","year":"2014","journal-title":"PLoS One"},{"key":"2021111619494204900_B14","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1136\/bmj.323.7305.157","article-title":"Systematic reviews in health care: systematic reviews of evaluations of diagnostic and screening tests","volume":"323","author":"Deeks","year":"2001","journal-title":"BMJ"},{"key":"2021111619494204900_B15","doi-asserted-by":"publisher","first-page":"1293","DOI":"10.1002\/sim.4780121403","article-title":"Combining independent studies of a diagnostic test into a summary roc curve: data-analytic approaches and some additional considerations","volume":"12","author":"Moses","year":"1993","journal-title":"Stat. Med."},{"key":"2021111619494204900_B16","doi-asserted-by":"publisher","first-page":"1830","DOI":"10.1373\/clinchem.2010.147850","article-title":"Plasma microRNA-122 as a biomarker for viral-, alcohol-, and chemical-related hepatic diseases","volume":"56","author":"Zhang","year":"2010","journal-title":"Clin. Chem."},{"key":"2021111619494204900_B17","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1002\/mc.20712","article-title":"Circulating microRNAs, mir-21, mir-122, and mir-223, in patients with hepatocellular carcinoma or chronic hepatitis","volume":"50","author":"Xu","year":"2011","journal-title":"Mol. Carcinog."},{"key":"2021111619494204900_B18","doi-asserted-by":"publisher","first-page":"e23937","DOI":"10.1371\/journal.pone.0023937","article-title":"Circulating microRNAs in patients with chronic hepatitis C and non-alcoholic fatty liver disease","volume":"6","author":"Cermelli","year":"2011","journal-title":"PLoS One"},{"key":"2021111619494204900_B19","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1111\/jvh.12001","article-title":"Sensitive detection of hepatocellular injury in chronic hepatitis C patients with circulating hepatocyte-derived microRNA-122","volume":"20","author":"van der Meer","year":"2013","journal-title":"J. Viral Hepat."},{"key":"2021111619494204900_B20","first-page":"5188","article-title":"Serum levels of microRNAs can specifically predict liver injury of chronic hepatitis B","volume":"18","author":"Zhang","year":"2012","journal-title":"World J. Gastroenterol."},{"key":"2021111619494204900_B21","doi-asserted-by":"publisher","first-page":"435476","DOI":"10.1155\/2014\/435476","article-title":"Severity of hepatitis C virus (genotype-3) infection positively correlates with circulating microRNA-122 in patients sera","volume":"2014","author":"Kumar","year":"2014","journal-title":"Dis. Markers"},{"key":"2021111619494204900_B22","doi-asserted-by":"publisher","first-page":"590","DOI":"10.7150\/ijms.11525","article-title":"Dysregulated serum microRNA expression profile and potential biomarkers in hepatitis C virus-infected patients","volume":"12","author":"Zhang","year":"2015","journal-title":"Int. J. Med. Sci."},{"key":"2021111619494204900_B23","first-page":"805","article-title":"Dysregulated serum microRNA expression profile and potential biomarkers in hepatitis C virus-infected patients","volume":"80","author":"Shaker","year":"2015","journal-title":"Med. J. Cairo Univ."},{"key":"2021111619494204900_B24","doi-asserted-by":"publisher","first-page":"9865","DOI":"10.1007\/s13277-016-4884-6","article-title":"Elevated serum microRNA-122\/222 levels are potential diagnostic biomarkers in egyptian patients with chronic hepatitis C but not hepatic cancer","volume":"37","author":"Motawi","year":"2016","journal-title":"Tumour Biol."},{"key":"2021111619494204900_B25","doi-asserted-by":"publisher","first-page":"21510","DOI":"10.1038\/srep21510","article-title":"Parallel expression profiling of hepatic and serum microRNA-122 associated with clinical features and treatment responses in chronic hepatitis C patients","volume":"6","author":"Butt","year":"2016","journal-title":"Sci. Rep."},{"key":"2021111619494204900_B26","doi-asserted-by":"publisher","first-page":"1806069","DOI":"10.1155\/2017\/1806069","article-title":"Detection of microRNA in hepatic cirrhosis and hepatocellular carcinoma in hepatitis C genotype-4 in Egyptian patients","volume":"2017","author":"Demerdash","year":"2017","journal-title":"BioMed. Res. Int."},{"key":"2021111619494204900_B27","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1186\/s13148-017-0404-9","article-title":"Identification and characterization of interferon signaling-related microRNAs in occult hepatitis B virus infection","volume":"9","author":"Wang","year":"2017","journal-title":"Clin. Epigenetics"},{"key":"2021111619494204900_B28","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.mgene.2018.08.005","article-title":"Assessment of serum level of miRNAs before and after treatment with sofosbuvir in Egyptian patients with chronic HCV infection","volume":"18","author":"Amr","year":"2018","journal-title":"Meta Gene"},{"key":"2021111619494204900_B29","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1007\/s12072-018-9871-0","article-title":"Plasma miRNA-122-5p and miRNA-151a-3p identified as potential biomarkers for liver injury among CHB patients with PNALT","volume":"12","author":"Cheng","year":"2018","journal-title":"Hepatol. Int."},{"key":"2021111619494204900_B30","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1186\/1471-2288-6-31","article-title":"Meta-disc: a software for meta-analysis of test accuracy data","volume":"6","author":"Zamora","year":"2006","journal-title":"BMC Med. Res. Method"},{"key":"2021111619494204900_B31","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1038\/35005107","article-title":"An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells","volume":"404","author":"Hammond","year":"2000","journal-title":"Nature"},{"key":"2021111619494204900_B32","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1016\/S0092-8674(02)00908-X","article-title":"Single-stranded antisense siRNAs guide target RNA cleavage in RNAi","volume":"110","author":"Martinez","year":"2002","journal-title":"Cell"},{"key":"2021111619494204900_B33","doi-asserted-by":"publisher","first-page":"106","DOI":"10.4161\/rna.1.2.1066","article-title":"Mir-122, a mammalian liver-specific microRNA, is processed from HCR mRNA and may downregulate the high affinity cationic amino acid transporter CAT-1","volume":"1","author":"Chang","year":"2004","journal-title":"RNA Biol."},{"key":"2021111619494204900_B34","doi-asserted-by":"publisher","first-page":"4511","DOI":"10.1096\/fj.11-187781","article-title":"A liver-specific microRNA binds to a highly conserved RNA sequence of hepatitis B virus and negatively regulates viral gene expression and replication","volume":"25","author":"Chen","year":"2011","journal-title":"FASEB J."},{"key":"2021111619494204900_B35","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1002\/hep.24809","article-title":"Loss of microRNA 122 expression in patients with hepatitis B enhances hepatitis B virus replication through cyclin g(1)-modulated p53 activity","volume":"55","author":"Wang","year":"2012","journal-title":"Hepatology"},{"key":"2021111619494204900_B36","doi-asserted-by":"publisher","first-page":"1881","DOI":"10.1073\/pnas.1213515110","article-title":"Competing and noncompeting activities of mir-122 and the 5\u2032 exonuclease XRN1 in regulation of hepatitis C virus replication","volume":"110","author":"Li","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021111619494204900_B37","doi-asserted-by":"publisher","first-page":"1577","DOI":"10.1126\/science.1113329","article-title":"Modulation of hepatitis C virus RNA abundance by a liver-specific microRNA","volume":"309","author":"Jopling","year":"2005","journal-title":"Science"},{"key":"2021111619494204900_B38","doi-asserted-by":"publisher","first-page":"3193","DOI":"10.1073\/pnas.1012464108","article-title":"Masking the 5\u2032 terminal nucleotides of the hepatitis C virus genome by an unconventional microRNA-target RNA complex","volume":"108","author":"Machlin","year":"2011","journal-title":"PNAS"},{"key":"2021111619494204900_B39","doi-asserted-by":"publisher","first-page":"941","DOI":"10.1073\/pnas.1112263109","article-title":"Stabilization of hepatitis C virus RNA by an Ago2-mir-122 complex","volume":"109","author":"Shimakami","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021111619494204900_B40","doi-asserted-by":"publisher","first-page":"1685","DOI":"10.1056\/NEJMoa1209026","article-title":"Treatment of HCV infection by targeting microRNA","volume":"368","author":"Janssen","year":"2013","journal-title":"N. Engl. J. Med."},{"key":"2021111619494204900_B41","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1111\/j.1478-3231.2010.02433.x","article-title":"Clinical significance and potential of hepatic microRNA-122 expression in hepatitis C","volume":"31","author":"Morita","year":"2011","journal-title":"Liver Int."},{"key":"2021111619494204900_B42","doi-asserted-by":"publisher","first-page":"1129","DOI":"10.1016\/S0895-4356(03)00177-X","article-title":"The diagnostic odds ratio: a single indicator of test performance","volume":"56","author":"Glas","year":"2003","journal-title":"J. Clin. Epidemiol."},{"key":"2021111619494204900_B43","doi-asserted-by":"publisher","first-page":"e41561","DOI":"10.1371\/journal.pone.0041561","article-title":"Comparing the microRNA spectrum between serum and plasma","volume":"7","author":"Wang","year":"2012","journal-title":"PLoS One"}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20190900\/854672\/bsr-2019-0900.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20190900\/854672\/bsr-2019-0900.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T15:33:44Z","timestamp":1637076824000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/39\/9\/BSR20190900\/220435\/Diagnostic-value-of-circulating-miRNA-122-for"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":43,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2019,9,30]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20190900","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,9]]},"published":{"date-parts":[[2019,9]]},"article-number":"BSR20190900"}}