{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T07:57:08Z","timestamp":1792396628085,"version":"4.4.0"},"reference-count":36,"publisher":"Portland Press Ltd.","issue":"6","license":[{"start":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T00:00:00Z","timestamp":1591660800000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,6,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In the present study, we examined the molecular mechanism of astragaloside IV (AS-IV) in high glucose (HG)-induced epithelial-to-mesenchymal transition (EMT) in renal proximal tubular epithelial cells (PTCs). NRK-52E cell viability and apoptosis were determined by the cell counting kit-8 (CCK-8) assay and flow cytometric analysis, respectively. Expressions of E-cadherin, N-cadherin, vimentin, and occludin were measured by Western blot, and those of E-cadherin and N-cadherin were additionally measured by immunofluorescence analysis. Transforming growth factor-\u03b21 (TGF-\u03b21) and \u03b1-smooth muscle actin (\u03b1-SMA) expressions were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. The expressions of Smad2, Smad3, phosphorylated-Smad2 (p-Smad2), and p-Smad3 were measured using Western blot. We found that AS-IV could recover NRK-52E cell viability and inhibit HG-induced cell apoptosis. TGF-\u03b21, \u03b1-SMA, Smad2, Smad3, p-Smad2, and p-Smad3 expressions were decreased in the AS-IV-treated groups compared with the HG group. Moreover, the expressions of E-cadherin and occludin were remarkably up-regulated and those of N-cadherin and vimentin were down-regulated in the AS-IV-treated groups compared with the HG group. Interestingly, the TGF-\u03b21 activator SRI-011381 hydrochloride had an antagonistic effect to AS-IV on HG-induced EMT behavior. In conclusion, AS-IV attenuates HG-induced EMT by inhibiting the TGF-\u03b2\/Smad pathway in renal PTCs.<\/jats:p>","DOI":"10.1042\/bsr20190987","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T07:06:41Z","timestamp":1591686401000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Astragaloside IV attenuates high glucose-induced EMT by inhibiting the TGF-\u03b2\/Smad pathway in renal proximal tubular epithelial cells"],"prefix":"10.1042","volume":"40","author":[{"given":"Ya-Ning","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Nephrology, Binzhou Medical University Hospital, Binzhou 256603, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Li","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Affiliated Huadu Hospital, Southern Medical University (People's Hospital of Huadu District), Guangzhou 510800, China"},{"name":"The Third School of Clinical Medicine, Southern Medical University, Guangzhou 510800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Yan","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Affiliated Huadu Hospital, Southern Medical University (People's Hospital of Huadu District), Guangzhou 510800, China"},{"name":"The Third School of Clinical Medicine, Southern Medical University, Guangzhou 510800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Xia","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Affiliated Huadu Hospital, Southern Medical University (People's Hospital of Huadu District), Guangzhou 510800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Guangzhou Hospital of Integrated Traditional and West Medicine, Guangzhou 510800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Ming","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of The Combination of Chinese and Western Medicine, Huadu District People\u2019s Hospital, Southern Medical University, Guangzhou 510800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Na","family":"Wang","sequence":"additional","affiliation":[{"name":"The Third School of Clinical Medicine, Southern Medical University, Guangzhou 510800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-Chun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Affiliated Huadu Hospital, Southern Medical University (People's Hospital of Huadu District), Guangzhou 510800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Affiliated Huadu Hospital, Southern Medical University (People's Hospital of Huadu District), Guangzhou 510800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3307-4028","authenticated-orcid":false,"given":"Hong-Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Affiliated Huadu Hospital, Southern Medical University (People's Hospital of Huadu District), Guangzhou 510800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9966-5023","authenticated-orcid":false,"given":"Yun-Fang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Affiliated Huadu Hospital, Southern Medical University (People's Hospital of Huadu District), Guangzhou 510800, China"},{"name":"The Third School of Clinical Medicine, Southern Medical University, Guangzhou 510800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,6,23]]},"reference":[{"key":"2021111620165410200_B1","first-page":"1319","article-title":"Advances in pathogenetic mechanisms of diabetic nephropathy","volume":"49","author":"Nicholas","year":"2003","journal-title":"Cell. 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