{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T04:41:42Z","timestamp":1789015302258,"version":"build-2803163510"},"reference-count":31,"publisher":"Portland Press Ltd.","issue":"1","license":[{"start":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T00:00:00Z","timestamp":1575244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,1,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Background: Hydrogen-rich saline (HRS) has been proven effective against ischemia\/reperfusion (I\/R) injury. However, knowledge on the underlying signaling events remain poor. Having recent highlight of microRNAs (miRNAs) in mediating intestinal I\/R injury, we hypothesized that HRS may protect intestine against I\/R injury by regulating miRNAs.<\/jats:p>\n                  <jats:p>Method: Mice were given intraperitoneal injection of saline or HRS once daily for five consecutive days before undergoing intestinal I\/R that was induced by 60-min ischemia followed by 180-min reperfusion of superior mesenteric artery. The intestine was collected for histopathological assay, miRNA microarray profiling, Real-Time PCR, and Western blotting. Next, miR-199a-3p mimics or inhibitors were transfected into IEC-6 cells to explore the relationship between HRS treatment and miR-199a-3p.<\/jats:p>\n                  <jats:p>Results: I\/R-induced mucosal injury and epithelial cells apoptosis were attenuated by HRS pretreatment. A total of 64 intestinal I\/R-responsive miRNAs were altered significantly by HRS pretreatment, in which we validated four novel miRNAs with top significance by Real-Time PCR, namely miR-199a-3p, miR-296-5p, miR-5126, and miR-6538. Particularly, miR-199a-3p was drastically increased by I\/R but reduced by HRS. Computational analysis predicts insulin-like growth factor (IGF)-1, mammalian target of rapamycin (mTOR), and phosphoinositide-3-kinase (PI3K) regulatory subunit 1 as targets of miR-199a-3p, suggesting involvement of the pro-survival pathway, IGF- 1\/PI3K\/Akt\/mTOR. In in vitro experiment, HRS treatment reduced miR-199a-3p level, increase IGF-1, PI3K and mTOR mRNA expression, restore IEC-6 cells viability, and this protective effects were reversed under miR-199a-3p mimics treatment.<\/jats:p>\n                  <jats:p>Conclusion: Collectively, miR-199a-3p may serve a key role in the anti-apoptotic mechanism of HRS that contributes to its protection of the intestine against I\/R injury.<\/jats:p>","DOI":"10.1042\/bsr20191043","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T14:30:03Z","timestamp":1575297003000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["MicroRNA files in the prevention of intestinal ischemia\/reperfusion injury by hydrogen rich saline"],"prefix":"10.1042","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5284-9159","authenticated-orcid":false,"given":"Weifeng","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanfen","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anshun","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziqing","family":"Hei","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianpei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinjie","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,1,24]]},"reference":[{"key":"2021111619561022400_B1","doi-asserted-by":"publisher","first-page":"1376","DOI":"10.1097\/00003246-199309000-00023","article-title":"Reperfusion injury after intestinal ischemia","volume":"21","author":"Schoenberg","year":"1993","journal-title":"Crit. 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