{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T13:22:54Z","timestamp":1791638574050,"version":"4.3.4"},"reference-count":20,"publisher":"Portland Press Ltd.","issue":"9","license":[{"start":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T00:00:00Z","timestamp":1569542400000},"content-version":"vor","delay-in-days":26,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study was to identify the drug resistance, resistance mechanism and the extended-spectrum \u03b2-lactamase (ESBLs) genotypes of Shigella flexneri (S. flexneri) in Jinan. Susceptibility tests were performed by MIC-determination. The genotypes of \u03b2-lactamase were identified using PCR and DNA sequencing. The resistance transfer ability of the ESBL-producing strains was examined by conjugation tests. A total of 105 S. flexneri isolates were collected, and 34 (32.4%) were ESBL-producing isolates. All ESBL-producing isolates were susceptible to cefoxitin and imipenem, and 35.3% isolates were resistant to ciprofloxacin. ESBL-producing isolates showed high level resistant to ampicillin (100%), cefotaxime (100%), tetracycline (100%), chloramphenicol (100%), trimethoprim\/sulfamethoxazole (100%), ceftazidime (73.5%) and cefepime (73.5%). Three types of \u03b2-lactamase genes (blaTEM, blaOXA and blaCTX-M) were identified in all ESBL-producing isolates, and the genotypes were confirmed as blaTEM-1 (23\/34), blaOXA-30 (34\/34), blaCTX-M-14 (9\/34) and blaCTX-M-15 (25\/34) by sequencing. In conclusion, the Shigella strains isolated in Jinan are cross-resistant and multi-drug resistant. The main genotypes of ESBLs are CTX-M-14 and CTX-M-15.<\/jats:p>","DOI":"10.1042\/bsr20191741","type":"journal-article","created":{"date-parts":[[2019,9,13]],"date-time":"2019-09-13T15:55:12Z","timestamp":1568390112000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Resistance characteristics of CTX-M type<i>Shigella flexneri<\/i>in China"],"prefix":"10.1042","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3381-0443","authenticated-orcid":false,"given":"Fengzhi","family":"Bian","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The Fourth People\u2019s Hospital of Jinan, Jinan, Shandong Province 250031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxiao","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Viral Infectious Diseases Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, Shandong Province 250014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmei","family":"Fu","sequence":"additional","affiliation":[{"name":"Jinan Blood Supply and Security Center of Shandong Province, Jinan, Shandong Province 250023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangying","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Jinan Infectious Disease Hospital Affiliated to Shandong University, Jinan, Shandong Province 250021, China"},{"name":"Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Taishan Medical University, Taian, Shandong Province 271016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuzhi","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Viral Infectious Diseases Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, Shandong Province 250014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4150-5841","authenticated-orcid":false,"given":"Yuguo","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The Fourth People\u2019s Hospital of Jinan, Jinan, Shandong Province 250031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2019,9,24]]},"reference":[{"key":"2021111619480733500_B1","doi-asserted-by":"publisher","first-page":"1126","DOI":"10.1093\/infdis\/159.6.1126","article-title":"Inoculum size in shigellosis and implications for expected mode of transmission","volume":"159","author":"DuPont","year":"1989","journal-title":"J. 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