{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T01:13:26Z","timestamp":1793236406626,"version":"4.5.0"},"reference-count":34,"publisher":"Portland Press Ltd.","issue":"2","license":[{"start":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T00:00:00Z","timestamp":1580083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,2,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Production of anti-sperm antibody (ASA) often suffers from autoimmune reaction against sperms in human infertility. The antibodies are measured in both blood and seminal plasma of males. Here, we reported a simple protein biochip methodology that takes advantage of a functionalized self-assembled monolayer modified by N-hydroxysuccinimide (NHS) and enables identification of anti-sperm antibody in Chinese male infertility. To validate this biochip platform, we immobilized purified sperm protein on the biochip surface and tested a variety of parameters in quality controls for the protein assay, respectively. Then, we analyzed serum samples from 368 patients with infertility and 116 healthy donors by means of this biochip simultaneously. We found that positive rate of serum ASA was 20.92% (77\/368) in the cases and 1.72% (2\/116) in the controls, respectively. Furthermore, we further corroborated the biochip assay in comparison with ELISA method. We found that both methods were compatible for the detection of serum ASA in the patients. In addition, a follow-up study for natural conception in ASA-positive and ASA-negative patients was conducted. The result showed a significant correlation between serum ASA expression and natural pregnancy rate 6.5% in ASA-positive patients while 18.9% in ASA-negative patients, indicating the potential roles of ASA in naturally reproductive processes.<\/jats:p>","DOI":"10.1042\/bsr20191769","type":"journal-article","created":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T11:30:02Z","timestamp":1580124602000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["A novel protein biochip screening serum anti-sperm antibody expression and natural pregnancy rate in a follow-up study in Chinese infertility"],"prefix":"10.1042","volume":"40","author":[{"given":"Feihong","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Medicine, Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengyun","family":"Cai","sequence":"additional","affiliation":[{"name":"Anhui Academic Institute of Biology, Haiguan Road K-1, Hefei 230088, Anhui, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Anhui Academic Institute of Biology, Haiguan Road K-1, Hefei 230088, Anhui, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqing","family":"Fan","sequence":"additional","affiliation":[{"name":"Reproductive and Genetic Hospital of CITIC Xiangya, Hunan 410008, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Song","sequence":"additional","affiliation":[{"name":"Anhui Academic Institute of Biology, Haiguan Road K-1, Hefei 230088, Anhui, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenshan","family":"Xu","sequence":"additional","affiliation":[{"name":"Anhui Academic Institute of Biology, Haiguan Road K-1, Hefei 230088, Anhui, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2056-0606","authenticated-orcid":false,"given":"Weidong","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Pathology, Anhui Medical University, Hefei 230032, Anhui, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,2,11]]},"reference":[{"key":"2021111620011619100_B1","first-page":"3443","article-title":"Infertility in men: recent advances and continuing controversies","volume":"84","author":"De Kretser","year":"1999","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"2021111620011619100_B2","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1136\/bmj.305.6854.609","article-title":"Evidence for decreasing quality of semen during past 50 years","volume":"305","author":"Carlsen","year":"1992","journal-title":"BMJ"},{"key":"2021111620011619100_B3","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.gde.2014.06.005","article-title":"Environmentally induced epigenetic transgenerational inheritance of male infertility","volume":"26","author":"Guerrero-Bosagna","year":"2014","journal-title":"Curr. Opin. Genet. Dev."},{"key":"2021111620011619100_B4","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1186\/1471-2458-10-24","article-title":"Decreasing sperm quality: a global problem?","volume":"10","author":"Merzenich","year":"2010","journal-title":"BMC Public Health"},{"key":"2021111620011619100_B5","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1289\/ehp.00108961","article-title":"The question of declining sperm density revisited: an analysis of 101 studies published 1934-1996","volume":"108","author":"Swan","year":"2000","journal-title":"Environ. Health Perspect."},{"key":"2021111620011619100_B6","doi-asserted-by":"publisher","first-page":"1391","DOI":"10.1007\/s10815-014-0311-3","article-title":"Association of common SNP rs1136410 in PARP1 gene with the susceptibility to male infertility with oligospermia","volume":"31","author":"Chen","year":"2014","journal-title":"J. Assist. Reprod. Genet."},{"key":"2021111620011619100_B7","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1111\/and.12073","article-title":"4977-bp mitochondrial DNA deletion in infertile patients with varicocele","volume":"46","author":"Gashti","year":"2014","journal-title":"Andrologia"},{"key":"2021111620011619100_B8","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1016\/j.cca.2012.03.002","article-title":"Gene transcripts in spermatozoa: markers of male infertility","volume":"413","author":"Li","year":"2012","journal-title":"Clin. Chim. Acta"},{"key":"2021111620011619100_B9","first-page":"s41","article-title":"Genetic dissection of mammalian fertility pathways","author":"Matzuk","year":"2002","journal-title":"Nat. Cell Biol."},{"key":"2021111620011619100_B10","doi-asserted-by":"publisher","first-page":"1614","DOI":"10.1126\/science.1226985","article-title":"Life in a contaminated world","volume":"337","author":"Guillette","year":"2012","journal-title":"Science"},{"key":"2021111620011619100_B11","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.mce.2011.10.004","article-title":"Environmentally induced epigenetic inheritance of phenotype and disease","volume":"354","author":"Guerrero-Bosagna","year":"2012","journal-title":"Mol. Cell. Endocrinol."},{"key":"2021111620011619100_B12","doi-asserted-by":"publisher","first-page":"3363","DOI":"10.1093\/humrep\/13.12.3363","article-title":"Survey of the diagnosis and management of antisperm antibodies","volume":"13","author":"Krapez","year":"1998","journal-title":"Hum. Reprod."},{"key":"2021111620011619100_B13","doi-asserted-by":"publisher","first-page":"799","DOI":"10.1016\/S0015-0282(98)00302-1","article-title":"Antisperm antibodies: etiology, pathogenesis, diagnosis, and treatment","volume":"70","author":"Mazumdar","year":"1998","journal-title":"Fertil. Steril."},{"key":"2021111620011619100_B14","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1111\/j.2047-2927.2013.00160.x","article-title":"Male fertility and varicocoele: role of immune factors","volume":"2","author":"Bozhedomov","year":"2014","journal-title":"Andrology"},{"key":"2021111620011619100_B15","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1016\/S0015-0282(16)42618-X","article-title":"Incidence of antisperm antibody levels and granulomas in men","volume":"28","author":"Alexander","year":"1977","journal-title":"Fertil. Steril."},{"key":"2021111620011619100_B16","doi-asserted-by":"publisher","first-page":"7071","DOI":"10.1007\/s00216-014-8151-7","article-title":"Same-single-cell analysis using the microfluidic biochip to reveal drug accumulation enhancement by an amphiphilic diblock copolymer drug formulation","volume":"406","author":"Khamenehfar","year":"2014","journal-title":"Anal. Bioanal. Chem."},{"key":"2021111620011619100_B17","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.cbpa.2015.10.013","article-title":"Applications in high-content functional protein microarrays","volume":"30","author":"Moore","year":"2016","journal-title":"Curr. Opin. Chem. Biol."},{"key":"2021111620011619100_B18","doi-asserted-by":"publisher","first-page":"750","DOI":"10.3390\/bios5040750","article-title":"A Simple Microfluidic Platform for Long-Term Analysis and Continuous Dual-Imaging Detection of T-Cell Secreted IFN-gamma and IL-2 on Antibody-Based Biochip","volume":"5","author":"Baganizi","year":"2015","journal-title":"Biosensors (Basel)"},{"key":"2021111620011619100_B19","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1016\/j.bios.2015.01.012","article-title":"Silicon nanonets for biological sensing applications with enhanced optical detection ability","volume":"68","author":"Serre","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"2021111620011619100_B20","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1093\/molehr\/7.2.113","article-title":"Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease","volume":"7","author":"Bohring","year":"2001","journal-title":"Mol. Hum. Reprod."},{"key":"2021111620011619100_B21","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1002\/j.1939-4640.1998.tb01987.x","article-title":"A panel of monoclonal antibodies against human sperm","volume":"19","author":"Tang","year":"1998","journal-title":"J. Androl."},{"key":"2021111620011619100_B22","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1111\/j.1600-0897.1989.tb00631.x","article-title":"Antibodies to sperm basic nuclear proteins detected in infertile patients by dot-immunobinding assay and by enzyme-linked immunosorbent assay","volume":"20","author":"Rousseaux-Prevost","year":"1989","journal-title":"Am. J. Reprod. Immunol."},{"key":"2021111620011619100_B23","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1530\/rep.1.00160","article-title":"Seminal plasma regulates endometrial cytokine expression, leukocyte recruitment and embryo development in the pig","volume":"128","author":"O\u2019Leary","year":"2004","journal-title":"Reproduction"},{"key":"2021111620011619100_B24","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1111\/j.1600-0897.1993.tb00611.x","article-title":"Relationship of sperm antibodies in women and men to human in vitro fertilization, cleavage, and pregnancy rate","volume":"30","author":"Chang","year":"1993","journal-title":"Am. J. Reprod. Immunol."},{"key":"2021111620011619100_B25","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1111\/j.1600-0897.1996.tb00505.x","article-title":"Cervical mucus anti-sperm antibodies: treatment with intrauterine insemination","volume":"35","author":"Kutteh","year":"1996","journal-title":"Am. J. Reprod. Immunol."},{"key":"2021111620011619100_B26","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1016\/S0015-0282(16)48139-2","article-title":"Prognosis of fertility in women with unexplained infertility and sperm agglutinins in the serum","volume":"42","author":"Rumke","year":"1984","journal-title":"Fertil. Steril."},{"key":"2021111620011619100_B27","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.jri.2010.09.002","article-title":"Anti-sperm antibody levels are not related to fertilization or pregnancy rates after IVF or IVF\/ICSI","volume":"88","author":"Zini","year":"2011","journal-title":"J. Reprod. Immunol."},{"key":"2021111620011619100_B28","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1016\/j.fertnstert.2006.02.089","article-title":"Association between sperm autoantibodies and enhanced embryo implantation rates during in vitro fertilization","volume":"86","author":"Clarke","year":"2006","journal-title":"Fertil. Steril."},{"key":"2021111620011619100_B29","doi-asserted-by":"publisher","first-page":"1288","DOI":"10.1093\/humrep\/der074","article-title":"Antisperm antibodies are not associated with pregnancy rates after IVF and ICSI: systematic review and meta-analysis","volume":"26","author":"Zini","year":"2011","journal-title":"Hum. Reprod."},{"key":"2021111620011619100_B30","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1016\/j.fertnstert.2003.09.084","article-title":"Clinical associations and mechanisms of action of antisperm antibodies","volume":"82","author":"Chiu","year":"2004","journal-title":"Fertil. Steril."},{"key":"2021111620011619100_B31","first-page":"52","article-title":"Anti-sperm IgA antibodies against the equatorial segment of the human spermatozoon are associated with impaired sperm penetration and subfertility","volume":"39","author":"Steen","year":"1994","journal-title":"Int. J. Fertil. Menopausal Stud."},{"key":"2021111620011619100_B32","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1038\/aja.2008.41","article-title":"A survey on the status of semen analysis in 118 laboratories in China","volume":"12","author":"Lu","year":"2010","journal-title":"Asian J. Androl."},{"key":"2021111620011619100_B33","doi-asserted-by":"publisher","first-page":"3413","DOI":"10.1007\/s00464-014-3614-7","article-title":"Antisperm antibodies and testicular blood flow after inguinal hernia mesh repair","volume":"28","author":"Stula","year":"2014","journal-title":"Surg. Endosc."},{"key":"2021111620011619100_B34","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.jri.2010.03.006","article-title":"Anti-sperm antibodies are not associated with sperm DNA damage: a prospective study of infertile men","volume":"85","author":"Zini","year":"2010","journal-title":"J. Reprod. Immunol."}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20191769\/867507\/bsr-2019-1769.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20191769\/867507\/bsr-2019-1769.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T18:59:37Z","timestamp":1722365977000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/40\/2\/BSR20191769\/221951\/A-novel-protein-biochip-screening-serum-anti-sperm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2,28]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20191769","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,2]]},"published":{"date-parts":[[2020,2]]},"article-number":"BSR20191769"}}