{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T13:34:12Z","timestamp":1758893652797,"version":"3.41.2"},"reference-count":27,"publisher":"Portland Press Ltd.","issue":"4","license":[{"start":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T00:00:00Z","timestamp":1586390400000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Purpose: The indispensable role of long non-coding RNAs (lncRNAs) in tumorigenesis has been increasingly reported. In the present study, LINC01694 was found to regulate the proliferation, invasion, as well as apoptosis in gallbladder cancer (GBC) cells through sponging miR-340-5p.<\/jats:p><jats:p>Methods: LINC01694 level in GBC cells was quantified by quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation, invasion, and apoptosis were determined by Cell Counting Kit-8 (CCK-8), Transwell, and flow cytometry, respectively. The expression of Sry-related high-mobility group box 4 (Sox4) was detected by Western blot (WB). The interaction between LINC01694 and miR-340-5p was measured by dual-luciferase reporter (DLR) assay, RNA immunoprecipitation (RIP) test, and RNA pull-down. Tumor formation was examined by in vivo experiment.<\/jats:p><jats:p>Results: qRT-PCR illustrated that cancerous tissues had higher LINC01694 than normal tissues. Survival analysis demonstrated that the prognosis of patients with high LINC01694 was significantly poorer than those with low LINC01694. Down-regulation of LINC01694 slowed down the proliferation and invasion in GBC cells and accelerated the apoptosis. DLR assay indicated that LINC01694 elevated Sox4 expression by regulating miR-340-5p. LINC01694 functioned as miR-340-5p sponge to inhibit Sox4 expression.<\/jats:p><jats:p>Conclusion: LINC01694 level is elevated in GBC by regulating miR-340-5p\/Sox4 axis, which indicates the poor prognosis of the patients.<\/jats:p>","DOI":"10.1042\/bsr20194444","type":"journal-article","created":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T10:58:30Z","timestamp":1586429910000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Knockdown of LINC01694 inhibits growth of gallbladder cancer cells via miR-340-5p\/Sox4"],"prefix":"10.1042","volume":"40","author":[{"given":"Lei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of General Surgery, Hanting District People\u2019s Hospital of Weifang, Weifang, Shandong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuexiang","family":"Yan","sequence":"additional","affiliation":[{"name":"General Branch 2, Weifang Yidu Central Hospital, Weifang, Shandong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guanyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medicine, Nanchang Univeristy, Nanchang, Jiangxi Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengxue","family":"Chen","sequence":"additional","affiliation":[{"name":"Dingtao District People\u2019s Hospital of Heze, Heze, Shandong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weihong","family":"Shen","sequence":"additional","affiliation":[{"name":"Dingtao District People\u2019s Hospital of Heze, Heze, Shandong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8811-2132","authenticated-orcid":false,"given":"Peixiang","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2020,4,24]]},"reference":[{"key":"2021111620080371700_B1","doi-asserted-by":"publisher","first-page":"967472","DOI":"10.1155\/2015\/967472","article-title":"Gallbladder cancer in the 21st century","volume":"2015","author":"Kanthan","year":"2015","journal-title":"J. Oncol."},{"key":"2021111620080371700_B2","doi-asserted-by":"publisher","first-page":"3978","DOI":"10.3748\/wjg.v23.i22.3978","article-title":"Gallbladder cancer epidemiology, pathogenesis and molecular genetics: recent update","volume":"23","author":"Sharma","year":"2017","journal-title":"World J. Gastroenterol."},{"key":"2021111620080371700_B3","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3322\/caac.21492","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA Cancer J. Clin."},{"key":"2021111620080371700_B4","first-page":"CD012233","article-title":"Transabdominal ultrasound and endoscopic ultrasound for diagnosis of gallbladder polyps","volume":"8","author":"Wennmacker","year":"2018","journal-title":"Cochrane Database Syst. Rev."},{"key":"2021111620080371700_B5","doi-asserted-by":"publisher","first-page":"744","DOI":"10.3748\/wjg.v24.i6.744","article-title":"Value of contrast-enhanced ultrasound in the differential diagnosis of gallbladder lesion","volume":"24","author":"Zhang","year":"2018","journal-title":"World J. Gastroenterol."},{"key":"2021111620080371700_B6","first-page":"2015","article-title":"Correlation of HMGB1 expression to progression and poor prognosis of adenocarcinoma and squamous cell\/adenosquamous carcinoma of gallbladder","volume":"7","author":"Shi","year":"2015","journal-title":"Am. J. Transl. Res."},{"key":"2021111620080371700_B7","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.3390\/cells8091015","article-title":"Long non-coding RNA in the pathogenesis of cancers","volume":"8","author":"Chi","year":"2019","journal-title":"Cells"},{"key":"2021111620080371700_B8","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1186\/s12943-019-1016-0","article-title":"Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy","volume":"18","author":"Cai","year":"2019","journal-title":"Mol. Cancer"},{"key":"2021111620080371700_B9","doi-asserted-by":"publisher","first-page":"1837","DOI":"10.15252\/embr.201744147","article-title":"LncRNA-PAGBC acts as a microRNA sponge and promotes gallbladder tumorigenesis","volume":"18","author":"Wu","year":"2017","journal-title":"EMBO Rep."},{"key":"2021111620080371700_B10","doi-asserted-by":"publisher","first-page":"1102","DOI":"10.1177\/1533034617733904","article-title":"Long noncoding RNA h19 inhibits cell viability, migration, and invasion via downregulation of IRS-1 in thyroid cancer cells","volume":"16","author":"Wang","year":"2017","journal-title":"Technol. Cancer Res. Treat."},{"key":"2021111620080371700_B11","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1210\/er.2014-1034","article-title":"From discovery to function: the expanding roles of long noncoding RNAs in physiology and disease","volume":"36","author":"Sun","year":"2015","journal-title":"Endocr. Rev."},{"key":"2021111620080371700_B12","doi-asserted-by":"publisher","first-page":"1924","DOI":"10.3390\/ijms20081924","article-title":"Long noncoding RNA and epithelial mesenchymal transition in cancer","volume":"20","author":"Gugnoni","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"2021111620080371700_B13","doi-asserted-by":"publisher","first-page":"a026492","DOI":"10.1101\/cshperspect.a026492","article-title":"Long noncoding RNAs: at the intersection of cancer and chromatin biology","volume":"7","author":"Schmitt","year":"2017","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"2021111620080371700_B14","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1016\/j.ccell.2016.03.010","article-title":"Long noncoding RNAs in cancer pathways","volume":"29","author":"Schmitt","year":"2016","journal-title":"Cancer Cell"},{"key":"2021111620080371700_B15","first-page":"127","article-title":"MicroRNA-338-3p suppresses metastasis of lung cancer cells by targeting the EMT regulator Sox4","volume":"6","author":"Li","year":"2016","journal-title":"Am. J. Cancer Res."},{"key":"2021111620080371700_B16","first-page":"1471","article-title":"American Joint Committee on Cancer cancer staging manual","volume-title":"Ann. Surg. Oncol.","author":"Edge","year":"2010"},{"key":"2021111620080371700_B17","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2021111620080371700_B18","first-page":"1365","article-title":"Bmi-1 in gallbladder carcinoma: Clinicopathology and mechanism of regulation of human gallbladder carcinoma proliferation","volume":"18","author":"Jiao","year":"2019","journal-title":"Oncol. Lett."},{"key":"2021111620080371700_B19","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1038\/nature20149","article-title":"Local regulation of gene expression by lncRNA promoters, transcription and splicing","volume":"539","author":"Engreitz","year":"2016","journal-title":"Nature"},{"key":"2021111620080371700_B20","doi-asserted-by":"crossref","first-page":"13914","DOI":"10.18632\/oncotarget.4227","article-title":"MicroRNA expression and its implications for diagnosis and therapy of gallbladder cancer","volume":"6","author":"Li","year":"2015","journal-title":"Oncotarget"},{"key":"2021111620080371700_B21","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/978-1-4939-3378-5_21","volume-title":"Analyzing miRNA\u2013lncRNA interactions[M]\/Long Non-Coding RNAs","author":"Paraskevopoulou","year":"2016"},{"key":"2021111620080371700_B22","doi-asserted-by":"publisher","first-page":"1310","DOI":"10.3390\/ijms19051310","article-title":"Noncoding RNA:RNA regulatory networks in cancer","volume":"19","author":"Chan","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"2021111620080371700_B23","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1452","article-title":"RNA versatility, flexibility, and thermostability for practice in RNA nanotechnology and biomedical applications","volume":"9","author":"Haque","year":"2018","journal-title":"Wiley Interdiscip. Rev. RNA"},{"key":"2021111620080371700_B24","first-page":"1094","article-title":"lncRNA-ATB functions as a competing endogenous RNA to promote YAP1 by sponging miR-590-5p in malignant melanoma","volume":"53","author":"Mou","year":"2018","journal-title":"Int. J. Oncol."},{"key":"2021111620080371700_B25","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1038\/cgt.2016.5","article-title":"Integrative analysis of microRNA and mRNA expression profiles in non-small-cell lung cancer","volume":"23","author":"Yang","year":"2016","journal-title":"Cancer Gene Ther."},{"key":"2021111620080371700_B26","first-page":"127","article-title":"MicroRNA-338-3p suppresses metastasis of lung cancer cells by targeting the EMT regulator Sox4","volume":"6","author":"Li","year":"2016","journal-title":"Am. J. Cancer Res."},{"key":"2021111620080371700_B27","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1186\/1746-1596-7-41","article-title":"Clinicopathological significance of SOX4 expression in primary gallbladder carcinoma","volume":"7","author":"Wang","year":"2012","journal-title":"Diagn. Pathol."}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20194444\/872554\/bsr-2019-4444.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20194444\/872554\/bsr-2019-4444.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T18:34:55Z","timestamp":1722710095000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/40\/4\/BSR20194444\/222622\/Knockdown-of-LINC01694-inhibits-growth-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4]]},"references-count":27,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,4,30]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20194444","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"type":"print","value":"0144-8463"},{"type":"electronic","value":"1573-4935"}],"subject":[],"published-other":{"date-parts":[[2020,4]]},"published":{"date-parts":[[2020,4]]},"article-number":"BSR20194444"}}