{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T15:45:17Z","timestamp":1776699917271,"version":"3.51.2"},"reference-count":34,"publisher":"Portland Press Ltd.","issue":"9","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Some microRNAs (miRs or miRNAs) have been reported to function as tumor suppressors in gallbladder cancer (GBC). However, the specific effect of miR-205-5p on GBC remains unclear. The objective of the present study was to unravel the effects of miR-205-5p on the drug resistance in GBC. For this purpose, the expression of miR-205-5p and protein kinase C \u03f5 (PRKCE) was quantified in the peripheral blood sample harvested from GBC patients and healthy volunteers. Then the relationship between miR-205-5p and PRKCE was validated. After isolating the GBC stem cells, ectopic expression and depletion experiments were conducted to analyze the effect of miR-205-5p and PRKCE on cell proliferation, drug resistance, apoptosis, and colony formation rate as well as the expression of apoptotic factors (Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), and cleaved caspase 3). Finally, the mouse xenograft model of GBC was established to verify the function of miR-205-5p in vivo. Intriguingly, our results manifested that miR-205-5p was down-regulated, while PRKCE was up-regulated in peripheral blood samples and stem cells of patients with GBC. Moreover, miR-205-5p targeted PRKCE and negatively regulated its expression. The overexpression of miR-205-5p or silencing of PRKCE inhibited the drug resistance, proliferation, and colony formation rate while promoting apoptosis of GBC stem cells. Additionally, the overexpression of miR-205-5p attenuated drug resistance to gemcitabine but promoted the gemcitabine-induced cell apoptosis by inhibiting the PRKCE in vivo. Overall, an intimate correlation between miR-205-5p and PRKCE is a key determinant of drug resistance of GBC stem cells, thus, suggesting a novel miR-205-5p-based clinical intervention target for GBC patients.<\/jats:p>","DOI":"10.1042\/bsr20194509","type":"journal-article","created":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T10:58:37Z","timestamp":1598957917000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Overexpression of microRNA-205-5p exerts suppressive effects on stem cell drug resistance in gallbladder cancer by down-regulating PRKCE"],"prefix":"10.1042","volume":"40","author":[{"given":"Guo-Feng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery, The Second Hospital of Jilin University, Changchun 130041, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia-Cheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery, The Second Hospital of Jilin University, Changchun 130041, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, The Second Hospital of Jilin University, Changchun 130041, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Dong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery, The Second Hospital of Jilin University, Changchun 130041, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3635-6917","authenticated-orcid":false,"given":"Ling","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, The Second Hospital of Jilin University, Changchun 130041, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2020,9,29]]},"reference":[{"key":"2021111620271458700_B1","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1111\/j.1440-1746.2011.07048.x","article-title":"Gallbladder cancer: can newer insights improve the outcome?","volume":"27","author":"Dutta","year":"2012","journal-title":"J. Gastroenterol. 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