{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T02:23:10Z","timestamp":1778638990265,"version":"3.51.4"},"reference-count":17,"publisher":"Portland Press Ltd.","issue":"3","license":[{"start":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T00:00:00Z","timestamp":1582675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,3,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A newly identified lncRNA designated as RP11-284P20.2 has been identified to be up-regulated in hepatocellular carcinoma (HCC), but its role in HCC remain poorly understood. Quantitative PCR and immunocytochemical analysis were performed using the HCC tissues to identify the potential interaction partners of RP11-284P20.2. Moreover, RP11-284P20.2 was knocked down in HCC cell lines, HepG2 and SMMC7721, to investigate the influence of this lncRNA on cell growth properties. Additionally, RNA fluorescence in situ hybridization and immunofluorescence, RNA immunoprecipitation, and RNA pull-down assays were performed to determine the interaction of RP11-284P20.2 with c-met mRNA and eukaryotic translation initiation factor 3b (EIF3b). Silencing RP11-284P20.2 inhibited cell viability, migration, invasion, and colony formation, and increased apoptosis. Overexpression of c-met abolished these effects of RP11-284P20.2 in HCC cells. Histopathological examination showed that HCC tissues with high RP11-284P20.2 expression had higher c-met protein level than that in HCC tissues with low RP11-284P20.2 expression. However, there was no positive correlation between the expression levels of RP11-284P20.2 and c-met mRNA. RP11-284P20.2 knockdown led to a decease in c-met protein expression level, but did not affect the c-met mRNA expression level. These data suggest that RP11-284P20.2 regulates c-met protein expression level, which is independent of c-Met mRNA expression level. It was also confirmed that RP11-284P20.2 has high affinity toward both c-met mRNA and EIF3b protein, and hence RP11-284P20.2 probably recruits EIF3b protein to c-met mRNA and further facilitates its translation. RP11-284P20.2 promotes cell proliferation and invasion in hepatocellular carcinoma by recruiting EIF3b to induce c-met protein synthesis.<\/jats:p>","DOI":"10.1042\/bsr20200297","type":"journal-article","created":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T11:39:10Z","timestamp":1582717150000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Long non-coding RNA RP11-284P20.2 promotes cell proliferation and invasion in hepatocellular carcinoma by recruiting EIF3b to induce c-met protein synthesis"],"prefix":"10.1042","volume":"40","author":[{"given":"Qin-Liang","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery, Zhongshan Hospital of Xiamen University, Hubin South Road, No. 201-209, Siming District, Xiamen 361004, China"},{"name":"Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, Zhongshan Hospital of Xiamen University, Hubin South Road, No. 201-209, Siming District, Xiamen 361004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Yin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Zhongshan Hospital of Xiamen University, Hubin South Road, No. 201-209, Siming District, Xiamen 361004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Zhongshan Hospital of Xiamen University, Hubin South Road, No. 201-209, Siming District, Xiamen 361004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng-Rong","family":"Xie","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, Zhongshan Hospital of Xiamen University, Hubin South Road, No. 201-209, Siming District, Xiamen 361004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fu-Qiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Zhongshan Hospital of Xiamen University, Hubin South Road, No. 201-209, Siming District, Xiamen 361004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun-Tong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Zhongshan Hospital of Xiamen University, Hubin South Road, No. 201-209, Siming District, Xiamen 361004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen-Yu","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Zhongshan Hospital of Xiamen University, Hubin South Road, No. 201-209, Siming District, Xiamen 361004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9827-0257","authenticated-orcid":false,"given":"Guo-Hui","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Zhongshan Hospital of Xiamen University, Hubin South Road, No. 201-209, Siming District, Xiamen 361004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2020,3,12]]},"reference":[{"key":"2021111620041280400_B1","doi-asserted-by":"publisher","first-page":"16018","DOI":"10.1038\/nrdp.2016.18","article-title":"Hepatocellular carcinoma","volume":"2","author":"Llovet","year":"2016","journal-title":"Nat. Rev. Dis. Primers"},{"key":"2021111620041280400_B2","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1002\/hep.29486","article-title":"Therapies for advanced stage hepatocellular carcinoma with macrovascular invasion or metastatic disease: A systematic review and meta-analysis","volume":"67","author":"Finn","year":"2018","journal-title":"Hepatology"},{"key":"2021111620041280400_B3","doi-asserted-by":"publisher","first-page":"1132","DOI":"10.1002\/hep.29496","article-title":"Recent developments of c-Met as a therapeutic target in hepatocellular carcinoma","volume":"67","author":"Bouattour","year":"2018","journal-title":"Hepatology"},{"key":"2021111620041280400_B4","doi-asserted-by":"publisher","first-page":"3695","DOI":"10.3748\/wjg.v24.i33.3695","article-title":"Updates on the hepatocyte growth factor\/c-Met axis in hepatocellular carcinoma and its therapeutic implications","volume":"24","author":"Garc\u00eda-Vilas","year":"2018","journal-title":"World J. Gastroenterol."},{"key":"2021111620041280400_B5","doi-asserted-by":"publisher","first-page":"E66","DOI":"10.3390\/biom10010066","article-title":"The emerging role of long non-coding rnas in the metastasis of hepatocellular carcinoma","volume":"10","author":"Chen","year":"2019","journal-title":"Biomolecules"},{"key":"2021111620041280400_B6","doi-asserted-by":"publisher","first-page":"2246","DOI":"10.1002\/1878-0261.12553","article-title":"lncRNA-PDPK2P promotes hepatocellular carcinoma progression through the PDK1\/AKT\/Caspase 3 pathway","volume":"13","author":"Pan","year":"2019","journal-title":"Mol. Oncol."},{"key":"2021111620041280400_B7","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.tcb.2018.11.002","article-title":"Tales of detailed poly(A) tails","volume":"29","author":"Nicholson","year":"2019","journal-title":"Trends Cell Biol."},{"key":"2021111620041280400_B8","doi-asserted-by":"publisher","first-page":"2977","DOI":"10.1074\/jbc.REV118.005594","article-title":"Polyadenylation and nuclear export of mRNAs","volume":"294","author":"Stewart","year":"2019","journal-title":"J. Biol. Chem."},{"key":"2021111620041280400_B9","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.bbagrm.2015.06.003","article-title":"Long noncoding RNAs: Re-writing dogmas of RNA processing and stability","volume":"1859","author":"Wilusz","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"2021111620041280400_B10","doi-asserted-by":"publisher","first-page":"18504","DOI":"10.1002\/jcp.28486","article-title":"Silencing of a novel lncRNA LOC105369748 suppresses the progression of hepatocellular carcinoma by sponging miR-5095 from MBD2","volume":"234","author":"E","year":"2019","journal-title":"J. Cell. Physiol."},{"key":"2021111620041280400_B11","doi-asserted-by":"publisher","first-page":"6045","DOI":"10.1002\/jcb.26802","article-title":"LncRNA FLVCR1-AS1 acts as miR-513c sponge to modulate cancer cell proliferation, migration, and invasion in hepatocellular carcinoma","volume":"119","author":"Zhang","year":"2018","journal-title":"J. Cell. Biochem."},{"key":"2021111620041280400_B12","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1002\/jcp.26779","article-title":"LncRNA DGCR5 represses the development of hepatocellular carcinoma by targeting the miR-346\/KLF14 axis","volume":"234","author":"Wang","year":"2018","journal-title":"J. Cell. Physiol."},{"key":"2021111620041280400_B13","doi-asserted-by":"publisher","first-page":"E570","DOI":"10.3390\/ijms19020570","article-title":"Alteration of epigenetic regulation by long noncoding RNAs in cancer","volume":"19","author":"Morlando","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"2021111620041280400_B14","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1016\/j.bbalip.2018.01.005","article-title":"Adiponectin AS lncRNA inhibits adipogenesis by transferring from nucleus to cytoplasm and attenuating Adiponectin mRNA translation","volume":"1863","author":"Cai","year":"2018","journal-title":"Biochim. Biophys. Acta Mol. Cell Biol. Lipids"},{"key":"2021111620041280400_B15","doi-asserted-by":"publisher","first-page":"265","DOI":"10.7150\/thno.36045","article-title":"Long noncoding RNA PiHL regulates p53 protein stability through GRWD1\/RPL11\/MDM2 axis in colorectal cancer","volume":"10","author":"Deng","year":"2020","journal-title":"Theranostics"},{"key":"2021111620041280400_B16","doi-asserted-by":"publisher","first-page":"1855","DOI":"10.1002\/cam4.2860","article-title":"Long non-coding RNA ARAP1-AS1 promotes tumorigenesis and metastasis through facilitating proto-oncogene c-Myc translation via dissociating PSF\/PTB dimer in cervical cancer","volume":"9","author":"Zhang","year":"2020","journal-title":"Cancer Med."},{"key":"2021111620041280400_B17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.canlet.2019.12.032","article-title":"Long noncoding RNA GMAN promotes hepatocellular carcinoma progression by interacting with eIF4B","volume":"473","author":"Xu","year":"2020","journal-title":"Cancer Lett."}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20200297\/869373\/bsr-2020-0297.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20200297\/869373\/bsr-2020-0297.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T20:42:11Z","timestamp":1637095331000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/40\/3\/BSR20200297\/222211\/Long-non-coding-RNA-RP11-284P20-2-promotes-cell"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3,27]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20200297","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,3]]},"published":{"date-parts":[[2020,3]]},"article-number":"BSR20200297"}}