{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T11:32:08Z","timestamp":1785843128043,"version":"3.56.0"},"reference-count":40,"publisher":"Portland Press Ltd.","issue":"6","license":[{"start":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T00:00:00Z","timestamp":1590537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,6,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Metastasis is the main cause of cancer-associated deaths, yet this complex process is still not well understood. Many studies have shown that acetate is involved in cancer metastasis, but the molecular mechanisms remain to be elucidated. In the present study, we first measured the effect of acetate on zinc finger transcriptional repressor SNAI1 and acetyl-CoA synthetase 2 (ACSS2) under glucose limitation in renal cell carcinoma cell lines, 786-O and ACHN. Then, RNA interference and overexpression of ACSS2 were used to detect the role of acetate on SNAI1 expression and cell migration. Finally, chromatin immunoprecipitation assay (ChIP) was used to investigate the regulatory mechanism of acetate on SNAI1 expression. The results showed that acetate increased the expressions of SNAI1 and ACSS2 under glucose limitation. ACSS2 knockdown significantly decreased acetate-induced SNAI1 expression and cell migration, whereas overexpression of ACSS2 increased SNAI1 level and histone H3K27 acetylation (H3K27ac). ChIP results revealed that acetate increased H3K27ac levels in regulatory region of SNAI1, but did not increase ACSS2-binding ability. Our study identified a novel inducer, acetate, which can promote SNAI1 expression by ACSS2-mediated histone acetylation in partly. This finding has important implication in treatment of metastatic cancers.<\/jats:p>","DOI":"10.1042\/bsr20200382","type":"journal-article","created":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T10:50:58Z","timestamp":1590576658000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Acetate promotes <i>SNAI1<\/i> expression by ACSS2-mediated histone acetylation under glucose limitation in renal cell carcinoma cell"],"prefix":"10.1042","volume":"40","author":[{"given":"Lv","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linying","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuxing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minghua","family":"Li","sequence":"additional","affiliation":[{"name":"Central Laboratory, Peking University Shenzhen Hospital, Shenzhen 518036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Central Laboratory, Peking University Shenzhen Hospital, Shenzhen 518036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1068-5926","authenticated-orcid":false,"given":"Xiaoqiang","family":"Guo","sequence":"additional","affiliation":[{"name":"Central Laboratory, Peking University Shenzhen Hospital, Shenzhen 518036, China"},{"name":"Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China"},{"name":"Xiaobei Medical Research Institute, Department of Physical Education, Shijiazhuang Vocational College of Technology, Shijiazhuang, Hebei 050081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,6,15]]},"reference":[{"key":"2021111620145225000_B1","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1016\/j.cell.2011.02.013","article-title":"Hallmarks of cancer: the next generation","volume":"144","author":"Hanahan","year":"2011","journal-title":"Cell"},{"key":"2021111620145225000_B2","doi-asserted-by":"publisher","first-page":"1559","DOI":"10.1126\/science.1203543","article-title":"A perspective on cancer cell metastasis","volume":"331","author":"Chaffer","year":"2011","journal-title":"Science"},{"key":"2021111620145225000_B3","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.ceb.2016.06.002","article-title":"Epithelial-mesenchymal transition (EMT) and metastasis: yes, no, maybe?","volume":"43","author":"Diepenbruck","year":"2016","journal-title":"Curr. 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