{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T20:33:54Z","timestamp":1788640434167,"version":"build-2803163510"},"reference-count":45,"publisher":"Portland Press Ltd.","issue":"10","license":[{"start":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T00:00:00Z","timestamp":1602115200000},"content-version":"vor","delay-in-days":7,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,10,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Bone morphogenetic protein-2 (BMP-2) is commonly applied in spinal surgery to augment spinal fusion. Nevertheless, its pro-inflammatory potential could induce dangerous side effects such as vascular hyper-permeability, posing the need for manners against this condition. The present study aims to investigate the protective effect of Zanthoxylum nitidum (ZN) on BMP-2-related hyperpermeability and inflammation on the human umbilical vein endothelial cells (HUVECs). The results revealed that, in a concentration-dependent manner, BMP-2 enhanced the production of pro-inflammatory cytokines, including interleukin (IL)-1\u03b1, IL-1\u03b2, and tumor necrosis factor-\u03b1, which were, however, suppressed by ZN. ZN inhibited BMP-2-induced inflammatory response by suppressing the phosphorylation of NF-\u03baBp65 and I\u03baB, and the abnormal nuclear translocation of p65. Moreover, the inhibited expression intercellular tight junction protein VE-cadherin and Occludin caused by BMP-2 was blocked by ZN. The hyper-permeability of HUVECs induced by BMP-2, as expressed as the higher fluorescent intensity of dextran, was also reversed by ZN. Overall, these findings demonstrated that ZN antagonized BMP-2-induced inflammation and hyperpermeability. It could be a therapeutic candidate for the treatment of BMP-2-induced side effects during spinal fusion.<\/jats:p>","DOI":"10.1042\/bsr20201098","type":"journal-article","created":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T13:07:40Z","timestamp":1602162460000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Zanthoxylum nitidum extract attenuates BMP-2-induced inflammation and hyperpermeability"],"prefix":"10.1042","volume":"40","author":[{"given":"Tao","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Spine Surgery, Shanghai East Hospital, Tongji University School of Medicine, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwen","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, Huashan Hospital, Fudan University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Inorganic Coating Materials CAS, Shanghai Institute of Ceramics, Chinese Academy of Sciences, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanjin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery, Shanghai East Hospital, Tongji University School of Medicine, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8916-5626","authenticated-orcid":false,"given":"Desheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery, Shanghai East Hospital, Tongji University School of Medicine, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,10,23]]},"reference":[{"key":"2021111620302824100_B1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/bs.vh.2015.06.002","article-title":"Bone Morphogenetic Proteins","volume":"99","author":"Carreira","year":"2015","journal-title":"Vitamins and Hormones"},{"key":"2021111620302824100_B2","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1089\/ten.teb.2015.0357","article-title":"A Review of the Clinical Side Effects of Bone Morphogenetic Protein-2","volume":"22","author":"James","year":"2016","journal-title":"Tissue Eng. - Part B. 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