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The objective of the present study was to evaluate the antioxidant, cytotoxic, thrombolytic, and antidiarrheal activities of carbon tetrachloride (CCl4) fraction of leaves of A. angustifolia (CTFAA) in different experimental models. Antioxidant activity was evaluated by using qualitative and quantitative assays, while antidiarrheal effects assessed with castor oil-induced diarrheal models in mice. The clot lysis and brine shrimp lethality bioassay were used to investigate the thrombolytic and cytotoxic activities, respectively. CTFAA showed antioxidant effects in all qualitative and quantitative procedures. The fraction produced dose-dependent and significant (P&amp;lt;0.05 and P&amp;lt;0.01) activities in castor oil-induced diarrheal models. Moreover, CTFAA significantly (P&amp;lt;0.05) demonstrated a 15.29% clot lysis effect in the thrombolytic test, and the brine shrimp lethality assay LC50 value was 424.16 \u03bcg\/ml bioassay. In conclusion, the current study showed CTFAA has significant antidiarrheal effects along with modest antioxidant and thrombolytic effects, and these data warrant further experiment to justify and include CTFAA as a supplement to mitigate the onset of diarrheal and cardiovascular disease.<\/jats:p>","DOI":"10.1042\/bsr20201110","type":"journal-article","created":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T07:19:15Z","timestamp":1592205555000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Evaluation of carbon tetrachloride fraction of <i>Actinodaphne angustifolia<\/i> Nees (Lauraceae) leaf extract for antioxidant, cytotoxic, thrombolytic and antidiarrheal properties"],"prefix":"10.1042","volume":"40","author":[{"given":"Mohammad\u00a0Najim","family":"Uddin","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Science & Engineering, International Islamic University Chittagong, Chattogram 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Towsif","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Science & Engineering, International Islamic University Chittagong, Chattogram 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad\u00a0Azharul","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Science & Engineering, International Islamic University Chittagong, Chattogram 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tawhidul\u00a0Amin","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Science & Engineering, International Islamic University Chittagong, Chattogram 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raihan\u00a0Uz","family":"Zaman","sequence":"additional","affiliation":[{"name":"Department of Thai Traditional Medicine, Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4707-3488","authenticated-orcid":false,"given":"Shofiul","family":"Azam","sequence":"additional","affiliation":[{"name":"Department of Applied Life Sciences and Integrated Bioscience, Graduate School, Konkuk University, Chungju 27478, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ATM\u00a0Mostafa","family":"Kamal","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Science & Engineering, International Islamic University Chittagong, Chattogram 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8216-1171","authenticated-orcid":false,"given":"Md.","family":"Jakaria","sequence":"additional","affiliation":[{"name":"The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,6,22]]},"reference":[{"key":"2021111620174136300_B1","first-page":"50","article-title":"Medicinal plant diversity and local healthcare among the people living in and around a conservation area of Northern Bangladesh","volume":"8","author":"Mukul","year":"2007","journal-title":"Int. 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