{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:02:14Z","timestamp":1785769334252,"version":"3.56.0"},"reference-count":30,"publisher":"Portland Press Ltd.","issue":"1","license":[{"start":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T00:00:00Z","timestamp":1603843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,1,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Background: Total saponins from Rubus parvifolius L. (TSRP) are the main bioactive fractions responsible for the anti-tumor activities. The work was aimed to evaluate the anti-tumor effect of TSRP in malignant melanoma (MM) in vitro and in vivo.<\/jats:p>\n               <jats:p>Methods and results: Anti-melanoma cell proliferation, invasion and migration effect of TSRP were detected in human MM A375 cells under the indicated time and dosages. In vivo anti-tumor effect of TSRP was measured in A375 xenograft immunodeficient nude mice. Sixty A375 xenografts were randomly divided into five groups: Vehicle, cyclophosphamide (CTX, 20 mg\/kg), TSRP (25 mg\/kg), TSRP (50 mg\/kg) and TSRP (100 mg\/kg) groups for 14 days\u2019 treatment. In addition, the melanoma metastasis in lung in vivo of TSRP was detected in A375 tail vein injection mice, and the histopathalogical analysis of the lung metastasis was detected by Hematoxylin\u2013Eosin (H&amp;E) staining. TSRP significantly inhibited the cell proliferation, invasion and migration of A375 in vitro at the indicated time and dosages. TSRP treatment effectively blocked the tumor growth in immunodeficient nude mice. In addition, TSRP also significantly inhibited the lung metastasis of melanoma.<\/jats:p>\n               <jats:p>Conclusion: The present study indicated that the TSRP has a remarkable anti-MM effect, which mainly through the inhibition of the cell invasion, migration and tumor metastasis.<\/jats:p>","DOI":"10.1042\/bsr20201178","type":"journal-article","created":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T09:50:51Z","timestamp":1603878651000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Total saponins from <i>Rubus parvifolius<\/i> L. inhibits cell proliferation, migration and invasion of malignant melanoma <i>in vitro<\/i> and <i>in vivo<\/i>"],"prefix":"10.1042","volume":"41","author":[{"given":"Jinfeng","family":"Cao","sequence":"first","affiliation":[{"name":"Ophthalmology Department, The Second Hospital of Jilin University, Changchun 130041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Zhao","sequence":"additional","affiliation":[{"name":"Otolaryngology Department, The Second Hospital of Jilin University, Changchun 130041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Ma","sequence":"additional","affiliation":[{"name":"Ophthalmology Department, The Second Hospital of Jilin University, Changchun 130041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Yang","sequence":"additional","affiliation":[{"name":"Ophthalmology Department, The Second Hospital of Jilin University, Changchun 130041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5228-5659","authenticated-orcid":false,"given":"Fuqiang","family":"Li","sequence":"additional","affiliation":[{"name":"Ophthalmology Department, The Second Hospital of Jilin University, Changchun 130041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2021,1,22]]},"reference":[{"key":"2021111620315591700_B1","doi-asserted-by":"publisher","first-page":"510","DOI":"10.4149\/neo_2016_403","article-title":"Malignant melanoma: diagnosis, treatment and cancer stem cells","volume":"63","author":"Kozovska","year":"2016","journal-title":"Neoplasma"},{"key":"2021111620315591700_B2","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1016\/j.jaad.2019.03.066","article-title":"Melanoma on chronically sun damaged skin: lentigo maligna and desmoplastic melanoma","volume":"81","author":"DeWane","year":"2019","journal-title":"J. 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