{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T04:27:50Z","timestamp":1767155270725,"version":"build-2238731810"},"reference-count":37,"publisher":"Portland Press Ltd.","issue":"6","license":[{"start":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T00:00:00Z","timestamp":1591056000000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,6,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Objective: To seek the clinical significance and regulatory mechanism of miR-135a and Rho-associated protein kinase 1 (ROCK1) in non-small cell lung cancer (NSCLC).<\/jats:p>\n                  <jats:p>Methods: NSCLC cells were purchased, and miR-135a-mimics, miR-135a-inhibitor, miR-NC, si-ROCK1 and Sh-ROCK1 were transfected into NSCLC cells HCC827 and NCI-H524. qRT-PCR and Western blot were used to detect the expression of miR-135a, ROCK1, Bax, Caspase3, Bcl-2, N-cadherin, vimentin and E-cadherin. MTT, scratch test, Transwell and flow cytometry were used to analyze the cell proliferation, migration, invasion and apoptosis.<\/jats:p>\n                  <jats:p>Results: miR-135a was low expressed in serum of NSCLC group, while ROCK1 was opposite. miR-135a low level or ROCK1 high level was associated with poor prognosis of NSCLC and lower 3-year OS. Over-expression of miR-135a and inhibition of ROCK1 expression could control malignant growth and diffusion of cells and expression of Bcl-2, N-cadherin and vimentin proteins, and promote apoptosis and expression of Bax, Caspase3 and E-cadherin proteins. After transfection of miR-135a-mimics+sh-ROCK1 to HCC827 and NCI-H524, the malignant proliferation and diffusion behavior of the cells were not different from those of the miR-NC group with no transfection sequence. The double luciferase report revealed that miR-135a has a targeting relationship with ROCK1.<\/jats:p>\n                  <jats:p>Conclusion: miR-135a is abnormally down-regulated in NSCLC. As a serum indicator, miR-135a has the potential to diagnose NSCLC and predict prognosis. The up-regulated expression of miR-135a protein can down-regulate the ROCK1 protein, inhibit the malignant proliferation, migration, invasion, EMT and other diffusion behaviors of NSCLC cells, and increase the apoptosis ability of cells.<\/jats:p>","DOI":"10.1042\/bsr20201276","type":"journal-article","created":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T08:01:01Z","timestamp":1591084861000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["miR-135a inhibits malignant proliferation and diffusion of non-small cell lung cancer cells by down-regulating ROCK1 protein"],"prefix":"10.1042","volume":"40","author":[{"given":"Yanan","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Oncology and Hematology, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaosong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Thyroid-Head & Neck Surgery, Jilin Cancer Hospital, Changchun, Jilin Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2974-7418","authenticated-orcid":false,"given":"Min","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2020,6,15]]},"reference":[{"key":"2021111620160556200_B1","first-page":"8153","article-title":"The association between NFKB1-94ins\/del ATTG polymorphism and non-small cell lung cancer risk in a Chinese Han population","volume":"8","author":"Wang","year":"2015","journal-title":"Int. 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