{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:19:03Z","timestamp":1772137143839,"version":"3.50.1"},"reference-count":9,"publisher":"Portland Press Ltd.","issue":"5","license":[{"start":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T00:00:00Z","timestamp":1588809600000},"content-version":"vor","delay-in-days":6,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,5,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Since 2002, \u03b2 coronaviruses (CoVs) have caused three zoonotic outbreaks, SARS-CoV in 2002, MERS-CoV in 2012, and the recent outbreak of SARS-CoV-2 late in 2019 (also named as COVID-19 or novel coronavirus 2019 or nCoV2019). Spike (S) protein, one of the structural proteins of this virus plays key role in receptor (ACE2) binding and thus virus entry. Thus, this protein has attracted scientists for detailed study and therapeutic targeting. As the nCoV2019 takes its course throughout the world, more and more sequence analyses are being done and genome sequences are being deposited in various databases. From India, two clinical isolates have been sequenced and the full genome has been deposited in GenBank. We have performed sequence analyses of the Spike protein of the Indian isolates and compared with that of the Wuhan, China (where the outbreak was first reported). While all the sequences of Wuhan isolates are identical, we found point mutations in the Indian isolates. Out of the two isolates, one was found to harbor a mutation in its receptor-binding domain (RBD) at position 407. At this site, arginine (a positively charged amino acid) was replaced by isoleucine (a hydrophobic amino acid that is also a C-\u03b2 branched amino acid). This mutation has been seen to change the secondary structure of the protein at that region and this can potentially alter receptor binding of the virus. Although this finding needs further validation and more sequencing, the information might be useful in rational drug designing and vaccine engineering.<\/jats:p>","DOI":"10.1042\/bsr20201312","type":"journal-article","created":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T07:13:59Z","timestamp":1588835639000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":84,"title":["A virus that has gone viral: amino acid mutation in S protein of Indian isolate of Coronavirus COVID-19 might impact receptor binding, and thus, infectivity"],"prefix":"10.1042","volume":"40","author":[{"given":"Priyanka","family":"Saha","sequence":"first","affiliation":[{"name":"Cancer Biology and Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C., Mullick Road, Jadavpur, Kolkata 700032, West Bengal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arup Kumar","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, North Bengal Medical College and Hospital, Sushrutanagar, Siliguri 734012, West Bengal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prem Prakash","family":"Tripathi","sequence":"additional","affiliation":[{"name":"Cell Biology and Physiology Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C., Mullick Road, Jadavpur, Kolkata 700032, West Bengal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit Kumar","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Cancer Biology and Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C., Mullick Road, Jadavpur, Kolkata 700032, West Bengal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4783-9295","authenticated-orcid":false,"given":"Upasana","family":"Ray","sequence":"additional","affiliation":[{"name":"Infectious Biology and Immunology Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C., Mullick Road, Jadavpur, Kolkata 700032, West Bengal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2020,5,15]]},"reference":[{"key":"2021111620112358100_B1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s40779-020-00240-0","article-title":"The origin, transmission and clinical therapies on coronavirus disease 2019 (COVID-19) outbreak\u2013an update on the status","volume":"7","author":"Guo","year":"2020","journal-title":"Military Med. Res."},{"key":"2021111620112358100_B2","article-title":"Fractal kinetics of Covid-19 pandemics (with update 3\/1\/20)","author":"Ziff","year":"2020","journal-title":"MedRxiv Preprint"},{"key":"2021111620112358100_B3","article-title":"Repurposing therapeutics for COVID-19: Supercomputer-based docking to the SARS-CoV-2 viral spike protein and viral spike protein-human ACE2 interface","volume-title":"ChemRxiv.","author":"Smith","year":"2020"},{"key":"2021111620112358100_B4","doi-asserted-by":"publisher","first-page":"254","DOI":"10.3390\/v12030254","article-title":"Preliminary identification of potential vaccine targets for the COVID-19 coronavirus (SARS-CoV-2) based on SARS-CoV immunological studies","volume":"12","author":"Ahmed","year":"2020","journal-title":"Viruses"},{"key":"2021111620112358100_B5","article-title":"Strong evolutionary convergence of receptor-binding protein spike between COVID-19 and SARS-related coronaviruses","author":"Wu","year":"2020","journal-title":"bioRxiv."},{"key":"2021111620112358100_B6","first-page":"410","article-title":"Role of changes in SARS-CoV-2 spike protein in the interaction with the human ACE2 receptor: an in silico analysis","volume":"19","author":"Ortega","year":"2020","journal-title":"EXCLI J."},{"key":"2021111620112358100_B7","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25726","article-title":"Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS-CoV-2","author":"Liu","year":"2020","journal-title":"J. Med. Virol."},{"key":"2021111620112358100_B8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41423-020-0400-4","article-title":"Characterization of the receptor-binding domain (RBD) of 2019 novel coronavirus: implication for development of RBD protein as a viral attachment inhibitor and vaccine","volume":"2020","author":"Wanbo","year":"2020","journal-title":"Cell. Mol. Immunol."},{"key":"2021111620112358100_B9","doi-asserted-by":"publisher","first-page":"12022","DOI":"10.1021\/bi00206a003","article-title":"Stabilizing and destabilizing effects of placing beta-branched amino acids in protein alpha-helices","volume-title":"Biochemistry","author":"Cornish","year":"1994"}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20201312\/879280\/bsr-2020-1312.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20201312\/879280\/bsr-2020-1312.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T15:46:58Z","timestamp":1637077618000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/40\/5\/BSR20201312\/223851\/A-virus-that-has-gone-viral-amino-acid-mutation-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5]]},"references-count":9,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5,29]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20201312","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.04.07.029132","asserted-by":"object"}]},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,5]]},"published":{"date-parts":[[2020,5]]},"article-number":"BSR20201312"}}