{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:54:41Z","timestamp":1791982481195,"version":"4.4.0"},"reference-count":22,"publisher":"Portland Press Ltd.","issue":"11","license":[{"start":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T00:00:00Z","timestamp":1603065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,11,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>To determine whether up-regulation of miR-1183 targeting the gene for anti-apoptotic factor, B-cell lymphoma 2 (BCL-2) contributes to apoptosis in patients with rheumatic heart disease (RHD). Peripheral blood samples were isolated for miR-1183 characterization. The function of miRNA-1183 in RHD using miRNA mimic on PBMCs and THP-1 cell models. The binding of miR-1183 and Bcl-2 gene was confirmed by luciferase activity test. We also measured expression levels of BCL-2 in heart valve tissue from patients with RHD using ELISA and immunohistochemistry. In silico analysis and reporter gene assays indicated that miR-1183 directly targets the mRNA encoding BCL-2. It is found that miR-1183 binds directly to the 3\u2032UTR of the BCL-2 mRNA and down-regulates the mRNA and protein levels of BCL-2. Overexpression of miR-1183 in RHD patients and cell lines down-regulated BCL-2 expression and induced apoptosis. With the progression of the disease, the expression of BCL-2 in the heart valve tissue of patients with RHD decreased. MiRNA-1183 is up-regulated in RHD and induces cardiac myocyte apoptosis through direct targeting and suppression of BCL-2, both of which might play important roles in RHD pathogenesis. During the compensatory period of RHD, up-regulated miR-1183 destroyed the balance of apoptosis proteins (Bax and BAK) in Bcl-2 family, enhance the apoptosis cascade reaction and reduce the anti apoptosis effect. The significantly higher expression levels of miR-1183 appear to play distinct roles in RHD pathogenesis by regulation BCL-2, possibly affecting myocardial apoptosis and remodeling in the context of RHD.<\/jats:p>","DOI":"10.1042\/bsr20201573","type":"journal-article","created":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T13:32:03Z","timestamp":1603114323000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["miRNA-1183-targeted regulation of <i>Bcl-<\/i>2 contributes to the pathogenesis of rheumatic heart disease"],"prefix":"10.1042","volume":"40","author":[{"given":"Ni","family":"Li","sequence":"first","affiliation":[{"name":"Department of Cardiothoracic Surgery, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China"},{"name":"Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linwen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guodong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lebo","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianqing","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5609-7196","authenticated-orcid":false,"given":"Guofeng","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,11,2]]},"reference":[{"key":"2021111620312707400_B1","first-page":"149","article-title":"Rheumatic heart disease associated with secondary renal amyloidosis","volume":"5","author":"Abdallah","year":"2012","journal-title":"Arab. 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