{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T18:28:01Z","timestamp":1793384881502,"version":"4.5.0"},"reference-count":31,"publisher":"Portland Press Ltd.","issue":"7","license":[{"start":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T00:00:00Z","timestamp":1594944000000},"content-version":"vor","delay-in-days":16,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>To assess the vaginal microbiome throughout full-term uncomplicated pregnancy, a longitudinal study was designed for 12 healthy women who had prepared to become pregnant and then delivered at term (38\u201342 weeks) without complications. The vaginal microbial community was studied at pre-pregnancy, 8\u201312, 24\u201328, 37\u201338 weeks of gestation, and puerperium, using hypervariable tag sequencing of the V3\u2013V4 region of the 16S rRNA gene. Sequencing produced approximately 10 million reads on the Illumina MiSeq. Members of the Firmicutes phyla were prevailing before and during pregnancy periods, and the proportion was quite as Proteobacteria until puerperium. Lactobacillus genus was abundant before and during pregnancy, but post-delivery vaginal microflora variety turned diverse. The species-level analysis revealed that a healthy vaginal microbiome before or during pregnancy was prominently dominated by Lactobacillus crispatus. Furthermore, PCoA analysis revealed for differences in the bacterial community composition between the two levels of Lactobacillus species in pre-pregnancy and pregnancy period (PC1 contribution of 58.46%, PC3 contribution of 8.64%). Based on the taxonomic and PCoA analysis, we found that L. crispatus was dominant in the vaginal microflora of healthy women before or during pregnancy, but at the puerperium, the status changed leading to decreased abundance of protective Lactobacillus species that made vaginal micro-ecological barrier vulnerable to diseases. Additionally, vaginal pH was an important environmental property affecting the vaginal microbial community.<\/jats:p>","DOI":"10.1042\/bsr20201766","type":"journal-article","created":{"date-parts":[[2020,7,24]],"date-time":"2020-07-24T08:36:11Z","timestamp":1595579771000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Vaginal microbiome analysis of healthy women during different periods of gestation"],"prefix":"10.1042","volume":"40","author":[{"given":"Dan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Xuan Wu Hospital, Capital Medical University, Beijing 100053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Zuo","family":"Chi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Xuan Wu Hospital, Capital Medical University, Beijing 100053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Beijing Tsinghua Chang Gung Hospital, Beijing 102218, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Beijing Tsinghua Chang Gung Hospital, Beijing 102218, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-rong","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Clinincal Laboratory, Xuan Wu Hospital, Capital Medical University, Beijing 100053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-yan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Xuan Wu Hospital, Capital Medical University, Beijing 100053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He-qin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Xuan Wu Hospital, Capital Medical University, Beijing 100053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7233-8826","authenticated-orcid":false,"given":"Qin-ping","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Xuan Wu Hospital, Capital Medical University, Beijing 100053, China"},{"name":"Department of Obstetrics and Gynecology, Beijing Tsinghua Chang Gung Hospital, Beijing 102218, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,7,24]]},"reference":[{"key":"2021111620215402100_B1","doi-asserted-by":"publisher","first-page":"1069","DOI":"10.1038\/ismej.2013.2","article-title":"Exploring the bovine rumen bacterial community from birth to adulthood","volume":"7","author":"Jami","year":"2013","journal-title":"ISME J."},{"key":"2021111620215402100_B2","doi-asserted-by":"publisher","first-page":"582","DOI":"10.3389\/fmicb.2016.00582","article-title":"Development of ruminal and fecal microbiomes are affected by weaning but not weaning strategy in dairy calves","volume":"7","author":"Meale","year":"2016","journal-title":"Front. 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