{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T09:45:03Z","timestamp":1784713503843,"version":"3.55.0"},"reference-count":30,"publisher":"Portland Press Ltd.","issue":"7","license":[{"start":{"date-parts":[[2020,6,23]],"date-time":"2020-06-23T00:00:00Z","timestamp":1592870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Anisodamine hydrobromide (AniHBr) is a Chinese medicine used to treat septic shock. However, whether AniHBr could ameliorate septic acute kidney injury and the underlying mechanism were not investigated. In the present study, 18 male Sprague-Dawley rats (200\u2013250 g) were randomly divided into control, lipopolysaccharide (LPS) and LPS+AniHBr groups. Rats were intravenously administrated with LPS or normal saline (for control). After 4 h, the rats were intravenously administrated with AniHBr (LPS+AniHBr) or normal saline at 4 h intervals. Hemodynamic parameters including blood pressure and heart rate were measured. The histopathologic evaluation of kidney tissues was performed. Lactate, creatine kinase, inflammatory cytokines and oxidative stress indicators were determined. Using Seahorse analysis, the metabolic analysis of mitochondrial stress and glycolytic stress in human renal proximal tubular epithelial cells treated with TNF-\u03b1 in the presence of AniHBr was performed. AniHBr administration significantly reduced serum creatine kinase and lactate following LPS treatment. AniHBr significantly improved hemodynamics in sepsis rats including increase in the mean atrial pressure and reduction in the heart rate. AniHBr significantly attenuated LPS-induced TNF-\u03b1, IL-6 and IL-1\u03b2 in serum, and LPS-induced TNF-\u03b1 and IL-1\u03b2 in renal tissues. The LPS-reduced SOD activity and LPS-increased MDA content were reversed by AniHBr. In vitro, TNF-\u03b1 increased mitochondrial oxygen consumption and glycolysis, but inhibited the ATP generation, which was reversed by AniHBr. Thus, AniHBr protects against the LPS-induced inflammatory cytokines, mitochondrial dysfunction and oxidative stress, and thus attenuates the LPS-induced acute kidney injury, showing AniHBr is a promising therapeutic drug for septic kidney injury.<\/jats:p>","DOI":"10.1042\/bsr20201812","type":"journal-article","created":{"date-parts":[[2020,6,23]],"date-time":"2020-06-23T10:29:37Z","timestamp":1592908177000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Protective effect of anisodamine hydrobromide on lipopolysaccharide-induced acute kidney injury"],"prefix":"10.1042","volume":"40","author":[{"given":"Feng","family":"Wan","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China"},{"name":"Chengdu No.1 Pharmaceutical Co., Ltd. Chengdu 610031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqiang","family":"Du","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueling","family":"He","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center, Sichuan University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5440-6194","authenticated-orcid":false,"given":"Ye","family":"Zeng","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,6,30]]},"reference":[{"key":"2021111620181965500_B1","doi-asserted-by":"publisher","first-page":"e107181","DOI":"10.1371\/journal.pone.0107181","article-title":"Epidemiology and outcome of severe sepsis and septic shock in intensive care units in mainland China","volume":"9","author":"Zhou","year":"2014","journal-title":"PLoS ONE"},{"key":"2021111620181965500_B2","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1007\/s40618-017-0742-7","article-title":"Anti-TNF-\u03b1 antibody alleviates insulin resistance in rats with sepsis-induced stress hyperglycemia","volume":"41","author":"Qu","year":"2018","journal-title":"J. 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