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However, its underlying mechanism remains unclear. The aim of the present study was to investigate the effect of NaB on globe transcriptome changes in DN. In our study, 8-week-old male db\/db mice suffering from DN were randomly divided into two groups: the DN+NaB group (DN mice treated with NaB, 5 g\/kg\/day) and the DN group (DN mice treated with saline). Further, normal db\/m mice were used as the normal control (NC) group. The blood glucose, body weight, urinary microalbumin and urinary creatinine of mice were measured for all three groups. Whole-transcriptome analysis was performed by RNA sequencing (RNA-Seq) to evaluate the profiling of long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs). Bioinformatics analysis was performed to predict the potential NaB-related lncRNAs and genes in DN. The expressions of lncRNAs and mRNAs were tested using the quantitative real-time polymerase chain reactions (qRT-PCRs) in renal tissues and mesangial cells treated with NaB. The results of the present study demonstrated that NaB ameliorated renal dysfunction in DN mice. Moreover, RNA-Seq results identified that some lncRNAs and mRNAs were reversely changed in the DN+NaB group in comparison to those in the DN group. Additionally, the integrated co-expression networks of NaB-related lncRNAs revealed that these lncRNAs interacted with 155 key mRNAs. Furthermore, the co-expression network of inflammation-related lncRNAs and mRNAs demonstrated that those reversed lncRNAs and mRNAs also play essential roles in the inflammatory response. In summary, the present study suggests that NaB ameliorates diabetes-induced renal dysfunction and regulates transcriptome changes in DN.<\/jats:p>","DOI":"10.1042\/bsr20203005","type":"journal-article","created":{"date-parts":[[2021,3,29]],"date-time":"2021-03-29T11:20:15Z","timestamp":1617016815000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["RNA-Seq analysis reveals critical transcriptome changes caused by sodium butyrate in DN mouse models"],"prefix":"10.1042","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7737-2134","authenticated-orcid":false,"given":"Hansen","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Genetics, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0062-517X","authenticated-orcid":false,"given":"Zheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Handeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Experimental Teaching Center, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiting","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2595-0961","authenticated-orcid":false,"given":"Yan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2021,4,9]]},"reference":[{"key":"2021111620423226100_B1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.2337\/dbi17-0013","article-title":"Diabetes in China: epidemiology and genetic risk factors and their clinical utility in personalized medication","volume":"67","author":"Hu","year":"2018","journal-title":"Diabetes"},{"key":"2021111620423226100_B2","doi-asserted-by":"publisher","first-page":"15018","DOI":"10.1038\/nrdp.2015.18","article-title":"Diabetic kidney disease","volume":"1","author":"Thomas","year":"2015","journal-title":"Nat. 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