{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T19:26:09Z","timestamp":1778786769424,"version":"3.51.4"},"reference-count":42,"publisher":"Portland Press Ltd.","issue":"4","license":[{"start":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T00:00:00Z","timestamp":1618531200000},"content-version":"vor","delay-in-days":15,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neonatal jaundice is a common disease that affects up to 60% of newborns. Herein, we performed a comparative analysis of the gut microbiome in neonatal jaundice and non-neonatal jaundice infants (NJIs) and identified gut microbial alterations in neonatal jaundice pre- and post-treatment. We prospectively collected 232 fecal samples from 51 infants at five time points (0, 1, 3, 6, and 12 months). Finally, 114 samples from 6 NJIs and 19 non-NJI completed MiSeq sequencing and analysis. We characterized the gut microbiome and identified microbial differences and gene functions. Meconium microbial diversity from NJI was decreased compared with that from non-NJI. The genus Gemella was decreased in NJI versus non-NJI. Eleven predicted microbial functions, including fructose 1,6-bisphosphatase III and pyruvate carboxylase subunit B, decreased, while three functions, including acetyl-CoA acyltransferase, increased in NJI. After treatments, the microbial community presented significant alteration-based \u03b2 diversity. The phyla Firmicutes and Actinobacteria were increased, while Proteobacteria and Fusobacteria were decreased. Microbial alterations were also analyzed between 6 recovered NJI and 19 non-NJI. The gut microbiota was unique in the meconium microbiome from NJI, implying that early gut microbiome intervention could be promising for the management of neonatal jaundice. Alterations of gut microbiota from NJI can be of great value to bolster evidence-based prevention against \u2018bacterial dysbiosis\u2019.<\/jats:p>","DOI":"10.1042\/bsr20210362","type":"journal-article","created":{"date-parts":[[2021,4,17]],"date-time":"2021-04-17T07:34:54Z","timestamp":1618644894000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Gut microbial alterations in neonatal jaundice pre- and post-treatment"],"prefix":"10.1042","volume":"41","author":[{"given":"Juan","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Quality Control, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Quality Control, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianlan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4972-4910","authenticated-orcid":false,"given":"Ang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China"},{"name":"Gene Hospital of Henan Province, Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Xin","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weining","family":"Lian","sequence":"additional","affiliation":[{"name":"Department of Quality Control, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyan","family":"Ren","sequence":"additional","affiliation":[{"name":"Shanghai Mobio Biomedical Technology Co., Ltd"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0798-3444","authenticated-orcid":false,"given":"Zhigang","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China"},{"name":"Gene Hospital of Henan Province, Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2021,4,30]]},"reference":[{"key":"2021111620443276800_B1","doi-asserted-by":"publisher","first-page":"c2409","DOI":"10.1136\/bmj.c2409","article-title":"Neonatal jaundice: summary of NICE guidance","volume":"340","author":"Rennie","year":"2010","journal-title":"BMJ"},{"key":"2021111620443276800_B2","doi-asserted-by":"publisher","first-page":"1691","DOI":"10.1093\/aje\/kwt248","article-title":"Neonatal bilirubin levels and childhood asthma in the US Collaborative Perinatal Project, 1959-1965","volume":"178","author":"Huang","year":"2013","journal-title":"Am. J. Epidemiol."},{"key":"2021111620443276800_B3","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1007\/s00592-011-0326-5","article-title":"Neonatal jaundice is associated with a small increase in the risk of childhood type 1 diabetes: a meta-analysis of observational studies","volume":"49","author":"McNamee","year":"2012","journal-title":"Acta Diabetol."},{"key":"2021111620443276800_B4","doi-asserted-by":"publisher","first-page":"6443","DOI":"10.1167\/iovs.14-14261","article-title":"Visuocortical function in infants with a history of neonatal jaundice","volume":"55","author":"Hou","year":"2014","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"2021111620443276800_B5","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1056\/NEJM200102223440807","article-title":"Neonatal hyperbilirubinemia","volume":"344","author":"Dennery","year":"2001","journal-title":"N. Engl. J. Med."},{"key":"2021111620443276800_B6","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1002\/hep.27927","article-title":"Absence of the intestinal microbiota exacerbates hepatobiliary disease in a murine model of primary sclerosing cholangitis","volume":"63","author":"Tabibian","year":"2016","journal-title":"Hepatology"},{"key":"2021111620443276800_B7","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.jnutbio.2018.09.016","article-title":"Association of serum bilirubin in newborns affected by jaundice with gut microbiota dysbiosis","volume":"63","author":"Zhou","year":"2019","journal-title":"J. Nutr. Biochem."},{"key":"2021111620443276800_B8","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1097\/MPG.0b013e31827a964b","article-title":"Breast milk jaundice: effect of bacteria present in breast milk and infant feces","volume":"56","author":"Tuzun","year":"2013","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"2021111620443276800_B9","first-page":"157","article-title":"Pharmacopoeia of the People\u2019s Republic of China (2010 Edition): a milestone in development of China\u2019s healthcare","volume":"02","author":"Qian","year":"2010","journal-title":"Chinese Herb. Med."},{"key":"2021111620443276800_B10","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1542\/peds.114.1.297","article-title":"Management of hyperbilirubinemia in the newborn infant 35 or more weeks of gestation","volume":"114","author":"American Academy of Pediatrics Subcommittee on Hyperbilirubinemia","year":"2004","journal-title":"Pediatrics"},{"key":"2021111620443276800_B11","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1136\/archdischild-2016-311556","article-title":"Jaundice in newborn babies under 28\u2005days: NICE guideline 2016 (CG98)","volume":"102","author":"Amos","year":"2017","journal-title":"Arch. Dis. Childhood Educ. Pract. Ed."},{"key":"2021111620443276800_B12","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1038\/nmeth.2604","article-title":"UPARSE: highly accurate OTU sequences from microbial amplicon reads","volume":"10","author":"Edgar","year":"2013","journal-title":"Nat. Methods"},{"key":"2021111620443276800_B13","doi-asserted-by":"publisher","first-page":"5261","DOI":"10.1128\/AEM.00062-07","article-title":"Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy","volume":"73","author":"Wang","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"2021111620443276800_B14","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1093\/bioinformatics\/btg412","article-title":"APE: analyses of phylogenetics and evolution in R language","volume":"20","author":"Paradis","year":"2004","journal-title":"Bioinformatics"},{"key":"2021111620443276800_B15","doi-asserted-by":"publisher","first-page":"e61217","DOI":"10.1371\/journal.pone.0061217","article-title":"phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data","volume":"8","author":"McMurdie","year":"2013","journal-title":"PLoS ONE"},{"key":"2021111620443276800_B16","doi-asserted-by":"publisher","first-page":"R60","DOI":"10.1186\/gb-2011-12-6-r60","article-title":"Metagenomic biomarker discovery and explanation","volume":"12","author":"Segata","year":"2011","journal-title":"Genome Biol."},{"key":"2021111620443276800_B17","doi-asserted-by":"publisher","first-page":"814","DOI":"10.1038\/nbt.2676","article-title":"Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences","volume":"31","author":"Langille","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"2021111620443276800_B18","doi-asserted-by":"publisher","first-page":"e1002358","DOI":"10.1371\/journal.pcbi.1002358","article-title":"Metabolic reconstruction for metagenomic data and its application to the human microbiome","volume":"8","author":"Abubucker","year":"2012","journal-title":"PLoS Comput. Biol."},{"key":"2021111620443276800_B19","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1111\/j.1651-2227.2006.tb02378.x","article-title":"WHO Child Growth Standards based on length\/height, weight and age","volume":"450","author":"WHO Multicentre Growth Reference Study Group","year":"2006","journal-title":"Acta Paediatr."},{"key":"2021111620443276800_B20","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1038\/s41424-018-0048-x","article-title":"Meconium microbiome associates with the development of neonatal jaundice","volume":"9","author":"Dong","year":"2018","journal-title":"Clin. Transl. Gastroenterol."},{"key":"2021111620443276800_B21","doi-asserted-by":"publisher","first-page":"1928","DOI":"10.1016\/S0140-6736(16)00081-7","article-title":"Gut bacteria dysbiosis and necrotising enterocolitis in very low birthweight infants: a prospective case-control study","volume":"387","author":"Warner","year":"2016","journal-title":"Lancet"},{"key":"2021111620443276800_B22","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1016\/j.jaci.2011.10.025","article-title":"Low diversity of the gut microbiota in infants with atopic eczema","volume":"129","author":"Abrahamsson","year":"2012","journal-title":"J. Allergy Clin. Immunol."},{"key":"2021111620443276800_B23","doi-asserted-by":"publisher","first-page":"F456","DOI":"10.1136\/fetalneonatal-2011-301373","article-title":"Gut microbial colonisation in premature neonates predicts neonatal sepsis","volume":"97","author":"Madan","year":"2012","journal-title":"Arch. Dis. Child. Fetal Neonatal Ed."},{"key":"2021111620443276800_B24","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1136\/gut.47.5.646","article-title":"Bifidobacterium strains from resident infant human gastrointestinal microflora exert antimicrobial activity","volume":"47","author":"Li\u00e9vin","year":"2000","journal-title":"Gut"},{"key":"2021111620443276800_B25","doi-asserted-by":"publisher","first-page":"325","DOI":"10.3920\/BM2014.0081","article-title":"Human intestinal mucosa-associated Lactobacillus and Bifidobacterium strains with probiotic properties modulate IL-10, IL-6 and IL-12 gene expression in THP-1 cells","volume":"6","author":"\u010citar","year":"2015","journal-title":"Benef. Microb."},{"key":"2021111620443276800_B26","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/j.earlhumdev.2015.08.013","article-title":"Human breast milk: a review on its composition and bioactivity","volume":"91","author":"Andreas","year":"2015","journal-title":"Early Hum. Dev."},{"key":"2021111620443276800_B27","doi-asserted-by":"publisher","DOI":"10.1111\/all.14736","article-title":"Breastfeeding promotes early neonatal regulatory T-cell expansion and immune tolerance of non-inherited maternal antigens","author":"Wood","year":"2021","journal-title":"Allergy"},{"key":"2021111620443276800_B28","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.cell.2013.12.016","article-title":"Microbiota-generated metabolites promote metabolic benefits via gut-brain neural circuits","volume":"156","author":"De Vadder","year":"2014","journal-title":"Cell"},{"key":"2021111620443276800_B29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1574-6968.2009.01514.x","article-title":"Diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine","volume":"294","author":"Louis","year":"2009","journal-title":"FEMS Microbiol. Lett."},{"key":"2021111620443276800_B30","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1128\/JB.01417-07","article-title":"Coupled ferredoxin and crotonyl coenzyme A (CoA) reduction with NADH catalyzed by the butyryl-CoA dehydrogenase\/Etf complex from Clostridium kluyveri","volume":"190","author":"Li","year":"2008","journal-title":"J. Bacteriol."},{"key":"2021111620443276800_B31","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1111\/j.1365-2036.2007.03562.x","article-title":"Review article: the role of butyrate on colonic function","volume":"27","author":"Hamer","year":"2008","journal-title":"Aliment. Pharmacol. Ther."},{"key":"2021111620443276800_B32","doi-asserted-by":"publisher","first-page":"2128","DOI":"10.1073\/pnas.0711093105","article-title":"The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features","volume":"105","author":"Seedorf","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021111620443276800_B33","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1007\/s10482-006-9121-7","article-title":"Relation between phylogenetic position, lipid metabolism and butyrate production by different Butyrivibrio-like bacteria from the rumen","volume":"91","author":"Paillard","year":"2007","journal-title":"Antonie Van Leeuwenhoek"},{"key":"2021111620443276800_B34","doi-asserted-by":"publisher","first-page":"1896","DOI":"10.1099\/ijs.0.65208-0","article-title":"Reclassification of Clostridium coccoides, Ruminococcus hansenii, Ruminococcus hydrogenotrophicus, Ruminococcus luti, Ruminococcus productus and Ruminococcus schinkii as Blautia coccoides gen. nov., comb. nov., Blautia hansenii comb. nov., Blautia hydrogenotrophica comb. nov., Blautia luti comb. nov., Blautia producta comb. nov., Blautia schinkii comb. nov. and description of Blautia wexlerae sp. nov., isolated from human faeces","volume":"58","author":"Liu","year":"2008","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"2021111620443276800_B35","doi-asserted-by":"publisher","first-page":"627","DOI":"10.3390\/d5030627","article-title":"Untangling the genetic basis of fibrolytic specialization by lachnospiraceae and ruminococcaceae in diverse gut communities","volume":"5","author":"Biddle","year":"2013","journal-title":"Diversity"},{"key":"2021111620443276800_B36","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1053\/j.gastro.2007.11.059","article-title":"Microbial influences in inflammatory bowel diseases","volume":"134","author":"Sartor","year":"2008","journal-title":"Gastroenterology"},{"key":"2021111620443276800_B37","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1038\/nature09922","article-title":"Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease","volume":"472","author":"Wang","year":"2011","journal-title":"Nature"},{"key":"2021111620443276800_B38","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1002\/9780470122983.ch2","article-title":"Mechanism of action of fructose 1,6-bisphosphatase","volume":"53","author":"Benkovic","year":"1982","journal-title":"Adv. Enzymol. Relat. Areas Mol. Biol."},{"key":"2021111620443276800_B39","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/j.metabol.2013.09.005","article-title":"Intestinal gluconeogenesis is crucial to maintain a physiological fasting glycemia in the absence of hepatic glucose production in mice","volume":"63","author":"Penhoat","year":"2014","journal-title":"Metabolism"},{"key":"2021111620443276800_B40","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1016\/j.cmet.2017.04.032","article-title":"Gut-brain glucose signaling in energy homeostasis","volume":"25","author":"Soty","year":"2017","journal-title":"Cell Metab."},{"key":"2021111620443276800_B41","doi-asserted-by":"publisher","first-page":"50","DOI":"10.3164\/jcbn.14-147","article-title":"Metabolomics changes in a rat model of obstructive jaundice: mapping to metabolism of amino acids, carbohydrates and lipids as well as oxidative stress","volume":"57","author":"Long","year":"2015","journal-title":"J. Clin. Biochem. Nutr."},{"key":"2021111620443276800_B42","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1186\/1475-2875-12-315","article-title":"Lipid peroxidation and antioxidant enzymes activity in Plasmodium vivax malaria patients evolving with cholestatic jaundice","volume":"12","author":"Fabbri","year":"2013","journal-title":"Malar. J."}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20210362\/908965\/bsr-2021-0362.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20210362\/908965\/bsr-2021-0362.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T07:40:36Z","timestamp":1698910836000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/41\/4\/BSR20210362\/228373\/Gut-microbial-alterations-in-neonatal-jaundice-pre"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4,30]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20210362","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,4]]},"published":{"date-parts":[[2021,4]]},"article-number":"BSR20210362"}}