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The present study isolated C. jejuni and S. typhimurim from fifty chicken carcass samples, all of which were acquired from different companies in Riyadh, Saudi Arabia. The identification of C. jejuni was performed phenotypically by using a hippurate test and genetically using a polymerase chain reaction with primers for 16S rRNA and hippurate hydrolase (hipO gene). For the dentification of S. typhimurim, a serological Widal test was carried out using serum anti-S. typhimurium antibodies. Strains were genetically detected using invA gene primers. The positive isolates for C. jejuni showed a specific molecular size of 1448 bp for 16S rRNA and 1148 bp for hipO genes. However, the positive isolates of the invA gene exhibited a specific molecular size at 244 bp using polymerase chain reaction (PCR). Comparing sequencing was performed with respect to the invA gene and the BLAST nucleotide isolates that were identified as Salmonella enterica subsp. enterica serovar typhimurium strain ST45, thereby producing a similarity of 100%. The testing identified C.jejuni for hippuricase, GenBank: Z36940.1. While many isolates of Salmonella spp. that contained the invA gene were not necessarily identified as S. typhimurim, the limiting factor for the Widal test used antiS. typhimurum antibodies. The multidrug resistance (MDR) of C. jejuni isolates in chickens was compared with the standard C. jejuni strain ATCC 22931. Similarly, S. typhimurium isolates were compared with the standard S. typhimurium strain ATCC 14028.<\/jats:p>","DOI":"10.1042\/bsr20211790","type":"journal-article","created":{"date-parts":[[2021,9,14]],"date-time":"2021-09-14T09:40:03Z","timestamp":1631612403000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Detection of <i>Campylobacter jejuni<\/i> and <i>Salmonella typhimurium<\/i> in chicken using PCR for virulence factor <i>hipO<\/i> and <i>inv<\/i>A genes (Saudi Arabia)"],"prefix":"10.1042","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3762-1498","authenticated-orcid":false,"given":"Khaloud\u00a0M.","family":"Alarjani","sequence":"first","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manal\u00a0F.","family":"Elkhadragy","sequence":"additional","affiliation":[{"name":"Biology Department, Faculty of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulrahman\u00a0H.","family":"Al-Masoud","sequence":"additional","affiliation":[{"name":"Food Science and Nutrition Department, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5387-8026","authenticated-orcid":false,"given":"Hany\u00a0M.","family":"Yehia","sequence":"additional","affiliation":[{"name":"Food Science and Nutrition Department, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia"},{"name":"Food Science and Nutrition Department, Faculty of Home Economics, Helwan University, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2021,9,22]]},"reference":[{"key":"2021111620530386200_B1","doi-asserted-by":"publisher","first-page":"820","DOI":"10.1089\/fpd.2015.1966","article-title":"Estimates of foodborne illness-related hospitalizations and deaths in Canada for 30 specified pathogens and unspecified agents","volume":"12","author":"Thomas","year":"2015","journal-title":"Foodborne Pathog. 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