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The present study attempts to reveal the effect and mechanism of XSSJS via regulating miR-29b-3p\/VEGFA axis against pathological angiogenesis in HF. In in vitro model, human embryonic kidney 293T cells were transfected with miR-29b-3p mimics, whereby the expression of miR-29b-3p was tested by real-time quantitative polymerase chain reaction (RT-qPCR), ensued by Luciferase assay determining the relationship between miR-29b-3p and vascular endothelial cell growth factor A (VEGFA). In addition, miR-29b-3p mimic transfected into the activated hepatic stellate cell T6 (HSC-T6). The Cell-Counting-Kit 8 (CCK8) and 5-Bromodeoxyuridine (BrdU) staining were first utilized to detect the antiproliferative efficiency of XSSJS following the XSSJS compound serum intervention, and then used to observe the expression of transforming growth factor-\u03b2 (TGF-\u03b2), VEGFA, platelet-derived growth factor (PDGF) via RT-PCR, Western blot (WB), and Immunofluorescence (IF) methods. During the in vivo model, XSSJS with boil-free granules were fed to Wistar rats with liver fibrosis caused by intraperitoneal injection of pig serum followed by the transfection of miR-29b-3p adeno-associated virus (AAV). Hematoxylin\u2013Eosin (HE) staining was used for histopathology assessment. The expression of miR-29b-3p, VEGFA, PDGF, TGF-\u03b2 have been investigated in liver tissue using RT-PCR, WB, IF. The results verified that XSSJS could up-regulate miR-29b-3p and suppress the expression of VEGFA, PDGA, and TGF-\u03b2. In mechanism, miR-29b-3p primarily targeted the 3\u2032UTR of VEGFA. In conclusion, XSSJS could modulate miR-29b-3p\/VEGFA axis to inhibit the pathological angiogenesis of HF.<\/jats:p>","DOI":"10.1042\/bsr20212241","type":"journal-article","created":{"date-parts":[[2022,2,4]],"date-time":"2022-02-04T09:47:06Z","timestamp":1643968026000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["XSSJS inhibits hepatic fibrosis by promoting the miR-29b-3p\/VEGFA axis <i>in vitro<\/i> and <i>in vivo<\/i>"],"prefix":"10.1042","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9633-3245","authenticated-orcid":false,"given":"Tianyao","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Fundamental Medical Science, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Fundamental Medical Science, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3148-5595","authenticated-orcid":false,"given":"Baojia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Fundamental Medical Science, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Long","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Fundamental Medical Science, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Fundamental Medical Science, Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Fundamental Medical Science, Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinyu","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Fundamental Medical Science, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2022,2,25]]},"reference":[{"key":"2022022508535308100_B1","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1172\/JCI24282","article-title":"Liver fibrosis","volume":"115","author":"Bataller","year":"2005","journal-title":"J. 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