{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T13:19:27Z","timestamp":1788873567526,"version":"build-2803163510"},"reference-count":30,"publisher":"Portland Press Ltd.","issue":"9","license":[{"start":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T00:00:00Z","timestamp":1693872000000},"content-version":"vor","delay-in-days":4,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institut Pasteur","award":["024009E"],"award-info":[{"award-number":["024009E"]}]},{"name":"Ministry of Higher Education and Scientific Research","award":["LR11IPT05"],"award-info":[{"award-number":["LR11IPT05"]}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,9,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Gut microbiota plays a key role in the regulation of metabolism and immunity. We investigated the profile of gut microbiota and the impact of dietary intake on gut bacterial distribution in diabetic and healthy Tunisian subjects, aiming to identify a dysbiotic condition, hence opening the way to restore eubiosis and facilitate return to health. In the present research, we enrolled 10 type 1 diabetic (T1D), 10 type 2 diabetic (T2D) patients and 13 healthy (H) subjects. Illumina Miseq technology was used to sequence V3-V4 hypervariable regions of bacterial 16SrRNA gene. Data were analyzed referring to QIIME 2 pipeline. RStudio software was used to explore the role of nutrition in gut bacterial distribution. At the phylum level, we identified an imbalanced gut microbiota composition in diabetic patients marked by a decrease in the proportion of Firmicutes and an increase in the abundance of Bacteroidetes compared with H subjects. We observed higher amounts of Fusobacteria and a decline in the levels of TM7 phyla in T1D patients compared with H subjects. However, we revealed a decrease in the proportions of Verrucomicrobia in T2D patients compared with H subjects. At the genus level, T2D subjects were more affected by gut microbiota alteration, showing a reduction in the relative abundance of Faecalibacterium, Akkermansia, Clostridium, Blautia and Oscillibacter, whereas T1D group shows a decrease in the proportion of Blautia. The gut bacteria distribution was mainly affected by fats and carbohydrates consumption. Gut microbiota composition was altered in Tunisian diabetic patients and affected by dietary habits.<\/jats:p>","DOI":"10.1042\/bsr20220803","type":"journal-article","created":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T16:31:13Z","timestamp":1693931473000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Gut microbiota profile and the influence of nutritional status on bacterial distribution in diabetic and healthy Tunisian subjects"],"prefix":"10.1042","volume":"43","author":[{"given":"Meriem","family":"Fassatoui","sequence":"first","affiliation":[{"name":"1Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, Tunis, Tunisia"},{"name":"2University of Tunis El Manar, Campus Universitaire Farhat Hached, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azadeh","family":"Saffarian","sequence":"additional","affiliation":[{"name":"3Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, INSERM U1202, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Mulet","sequence":"additional","affiliation":[{"name":"3Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, INSERM U1202, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henda","family":"Jamoussi","sequence":"additional","affiliation":[{"name":"1Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, Tunis, Tunisia"},{"name":"4Research Unit on Obesity UR18ES01, Faculty of Medicine, University Tunis El Manar, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amel","family":"Gamoudi","sequence":"additional","affiliation":[{"name":"5Department of Nutritional Diseases A. National Institute of Nutrition and Food Technology, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosra","family":"Ben Halima","sequence":"additional","affiliation":[{"name":"1Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meriem","family":"Hechmi","sequence":"additional","affiliation":[{"name":"1Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, Tunis, Tunisia"},{"name":"6University of Carthage, National Institute of Applied Science and Technology, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Abdelhak","sequence":"additional","affiliation":[{"name":"1Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, Tunis, Tunisia"},{"name":"2University of Tunis El Manar, Campus Universitaire Farhat Hached, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelmajid","family":"Abid","sequence":"additional","affiliation":[{"name":"1Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe\u00a0J.","family":"Sansonetti","sequence":"additional","affiliation":[{"name":"3Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, INSERM U1202, Institut Pasteur, Paris, France"},{"name":"7Chaire de Microbiologie et Maladies Infectieuses, Coll\u00e8ge de France, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Pedron","sequence":"additional","affiliation":[{"name":"3Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, INSERM U1202, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rym","family":"Kefi","sequence":"additional","affiliation":[{"name":"1Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, Tunis, Tunisia"},{"name":"2University of Tunis El Manar, Campus Universitaire Farhat Hached, Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2023,9,12]]},"reference":[{"key":"2023091208445820500_B1","volume-title":"IDF Diabetes Atlas 8th edition; 2017","author":"Atlas","year":"2016","edition":"Eight Edition"},{"key":"2023091208445820500_B2","doi-asserted-by":"publisher","first-page":"17737","DOI":"10.3748\/wjg.v20.i47.17737","article-title":"Intestinal microbiota and type 2 diabetes: from mechanism insights to therapeutic perspective","volume":"20","author":"Han","year":"2014","journal-title":"World J. Gastroenterol."},{"key":"2023091208445820500_B3","volume-title":"IDF Diabetes Atlas","author":"International Diabetes Federation","year":"2017","edition":"8th edition"},{"key":"2023091208445820500_B4","doi-asserted-by":"publisher","first-page":"109119","DOI":"10.1016\/j.diabres.2021.109119","article-title":"IDF Diabetes Atlas: Global, regional and country-level diabetes prevalence estimates for 2021 and projections for 2045","volume":"183","author":"Sun","year":"2022","journal-title":"Diabetes Res. Clin. Pract."},{"key":"2023091208445820500_B5","volume-title":"IDF Diabetes Atlas","author":"International Diabetes Federation","year":"2021","edition":"10th edition"},{"key":"2023091208445820500_B6","doi-asserted-by":"publisher","first-page":"901308","DOI":"10.1155\/2014\/901308","article-title":"Gut microbioma population: an indicator really sensible to any change in age, diet, metabolic syndrome, and life-style","volume":"2014","author":"Annalisa","year":"2014","journal-title":"Mediators Inflamm."},{"key":"2023091208445820500_B7","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1038\/nrg.2017.63","article-title":"Human genetic variation and the gut microbiome in disease","volume":"18","author":"Hall","year":"2017","journal-title":"Nat. Rev. Genet."},{"key":"2023091208445820500_B8","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1177\/0884533611436116","article-title":"Effects of gut microbes on nutrient absorption and energy regulation","volume":"27","author":"Krajmalnik-Brown","year":"2012","journal-title":"Nutr. Clin. Pract."},{"key":"2023091208445820500_B9","doi-asserted-by":"publisher","first-page":"358","DOI":"10.14310\/horm.2002.1571","article-title":"Beyond gut microbiota: understanding obesity and type 2 diabetes","volume":"14","author":"Lau","year":"2015","journal-title":"Hormones (Athens)"},{"key":"2023091208445820500_B10","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/J.ENG.2017.01.008","article-title":"The human microbiota in health and disease","volume":"3","author":"Wang","year":"2017","journal-title":"Engineering"},{"key":"2023091208445820500_B11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13099-021-00446-0","article-title":"Gut microbiota influence in type 2 diabetes mellitus (T2DM)","volume":"13","author":"Cunningham","year":"2021","journal-title":"Gut Pathogens"},{"key":"2023091208445820500_B12","doi-asserted-by":"publisher","first-page":"e3043","DOI":"10.1002\/dmrr.3043","article-title":"Gut microbiome in type 1 diabetes: A comprehensive review","volume":"34","author":"Zheng","year":"2018","journal-title":"Diabetes Metab. Res. Rev."},{"key":"2023091208445820500_B13","doi-asserted-by":"publisher","first-page":"10","DOI":"10.3945\/ajcn.116.136051","article-title":"The role of metabolism (and the microbiome) in defining the clinical efficacy of dietary flavonoids","volume":"105","author":"Cassidy","year":"2016","journal-title":"Am. J. Clin. Nutr."},{"key":"2023091208445820500_B14","doi-asserted-by":"publisher","first-page":"1151","DOI":"10.1126\/science.aao5774","article-title":"Gut bacteria selectively promoted by dietary fibers alleviate type 2 diabetes","volume":"359","author":"Zhao","year":"2018","journal-title":"Science"},{"key":"2023091208445820500_B15","doi-asserted-by":"publisher","first-page":"890","DOI":"10.3389\/fmicb.2018.00890","article-title":"Shifts on gut microbiota associated to Mediterranean diet adherence and specific dietary intakes on general adult population","volume":"9","author":"Garcia-Mantrana","year":"2018","journal-title":"Front. Microbiol."},{"key":"2023091208445820500_B16","first-page":"01","article-title":"Dietary fat and gut microbiota: mechanisms involved in obesity control","volume":"59","author":"Coelho","year":"2018","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"2023091208445820500_B17","doi-asserted-by":"publisher","first-page":"BSR20182348","DOI":"10.1042\/BSR20182348","article-title":"Gut microbiota imbalances in Tunisian participants with type 1 and type 2 diabetes mellitus","volume":"39","author":"Fassatoui","year":"2019","journal-title":"Biosci. Rep."},{"key":"2023091208445820500_B18","doi-asserted-by":"publisher","first-page":"1056","DOI":"10.1186\/s12864-015-2265-y","article-title":"Improved taxonomic assignment of human intestinal 16S rRNA sequences by a dedicated reference database","volume":"16","author":"Ritari","year":"2015","journal-title":"BMC Genomics"},{"key":"2023091208445820500_B19","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1016\/j.mad.2016.12.013","article-title":"The gut microbiota of centenarians: signatures of longevity in the gut microbiota profile","volume":"165","author":"Biagi","year":"2017","journal-title":"Mech. Ageing Dev."},{"key":"2023091208445820500_B20","doi-asserted-by":"publisher","first-page":"214","DOI":"10.3389\/fmicb.2017.00214","article-title":"Gut microbial diversity assessment of Indian type-2-diabetics reveals alterations in eubacteria, archaea, and eukaryotes","volume":"8","author":"Bhute","year":"2017","journal-title":"Front. Microbiol."},{"key":"2023091208445820500_B21","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/s13059-018-1616-9","article-title":"Population structure of human gut bacteria in a diverse cohort from rural Tanzania and Botswana","volume":"20","author":"Hansen","year":"2019","journal-title":"Genome Biol."},{"key":"2023091208445820500_B22","doi-asserted-by":"publisher","first-page":"9523","DOI":"10.1038\/s41598-017-10034-5","article-title":"Cross sectional evaluation of the gut-microbiome metabolome axis in an Italian cohort of IBD patients","volume":"7","author":"Santoru","year":"2017","journal-title":"Sci. Rep."},{"key":"2023091208445820500_B23","doi-asserted-by":"publisher","first-page":"1569","DOI":"10.1099\/jmm.0.47719-0","article-title":"Intestinal TM7 bacterial phylogenies in active inflammatory bowel disease","volume":"57","author":"Kuehbacher","year":"2008","journal-title":"J. Med. Microbiol."},{"key":"2023091208445820500_B24","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1055\/s-0042-107792","article-title":"Gut microbiota as a target in the pathogenesis of metabolic disorders: a new approach to novel therapeutic agents","volume":"48","author":"Ejtahed","year":"2016","journal-title":"Horm. Metab. Res."},{"key":"2023091208445820500_B25","doi-asserted-by":"publisher","first-page":"102590","DOI":"10.1016\/j.ebiom.2019.11.051","article-title":"Role of gut microbiota in type 2 diabetes pathophysiology","volume":"51","author":"Gurung","year":"2020","journal-title":"EBioMedicine"},{"key":"2023091208445820500_B26","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.aninu.2021.11.005","article-title":"Gut microbiota-derived short chain fatty acids are potential mediators in gut inflammation","volume":"8","author":"Akhtar","year":"2021","journal-title":"Animal Nutr."},{"key":"2023091208445820500_B27","doi-asserted-by":"publisher","first-page":"103293","DOI":"10.1016\/j.ebiom.2021.103293","article-title":"Short chain fatty acids and its producing organisms: an overlooked therapy for IBD?","volume":"66","author":"Deleu","year":"2021","journal-title":"EBioMedicine"},{"key":"2023091208445820500_B28","doi-asserted-by":"publisher","first-page":"157","DOI":"10.3389\/fnut.2019.00157","article-title":"Plant-based fat, dietary patterns rich in vegetable fat and gut microbiota modulation","volume":"6","author":"Muralidharan","year":"2019","journal-title":"Front. Nutrition"},{"key":"2023091208445820500_B29","doi-asserted-by":"publisher","first-page":"e1194","DOI":"10.1002\/mbo3.1194","article-title":"Low-carbohydrate high-fat weight reduction diet induces changes in human gut microbiota","volume":"10","author":"Jaagura","year":"2021","journal-title":"MicrobiologyOpen"},{"key":"2023091208445820500_B30","doi-asserted-by":"publisher","first-page":"1278","DOI":"10.3390\/antiox10081278","article-title":"Vitamin C supplementation in healthy individuals leads to shifts of bacterial populations in the gut\u2014a pilot study","volume":"10","author":"Otten","year":"2021","journal-title":"Antioxidants"}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20220803\/949926\/bsr-2022-0803.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20220803\/949926\/bsr-2022-0803.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T08:45:18Z","timestamp":1694508318000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/43\/9\/BSR20220803\/233493\/Gut-microbiota-profile-and-the-influence-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9]]},"references-count":30,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2023,9,27]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20220803","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,9]]},"published":{"date-parts":[[2023,9]]},"article-number":"BSR20220803"}}