{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T19:00:16Z","timestamp":1785783616935,"version":"3.56.0"},"reference-count":99,"publisher":"Portland Press Ltd.","issue":"5","license":[{"start":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T00:00:00Z","timestamp":1680739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,5,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The ability to control stem cell function is the key to stem cell-based therapy and living tissue regeneration. In natural conditions, histone deacetylases (HDAC) are regarded as the important defining epigenetic reprogramming for stem cell differentiation. To date, human adipose-derived stem cells (hADSCs) have been widely utilised for bone tissue engineering applications. The present study aimed to examine the effect of a novel HDAC2&amp;3-selective inhibitor, MI192, on hADSCs epigenetic reprogramming for regulating its osteogenic potential in vitro. The results confirmed that MI192 treatment reduced the hADSCs viability in a time and dose-dependent manner. The optimal concentration and pre-treatment time of MI192 for hADSCs osteogenic induction was 30 \u03bcM and 2 days representatively. A quantitative biochemical assay confirmed that the pre-treatment with MI192 (30 \u03bcM) for 2 days significantly enhanced hADSCs alkaline phosphatase (ALP) specific activity (P&amp;lt;0.05) compared with that of the valproic acid (VPA) pre-treatment group. Real-time PCR analysis revealed that MI192 pre-treatment up-regulated hADSCs gene expressions of osteogenic markers (e.g., Runx2, Col1, and OCN) under the osteogenic induction. DNA flow cytometric analysis indicated that two days\u2019 pre-treatment with MI192 (30 \u03bcM) resulted in G2\/M arrest in hADSCs and this G2\/M arrest was reversible. Our results suggest that MI192 is capable of epigenetic reprogramming of hADSCs via HDAC inhibition for controlling the cell cycle, resulting in enhancing hADSCs osteogenic differentiation, which indicates the potential of using MI192 for promoting bone tissue regeneration.<\/jats:p>","DOI":"10.1042\/bsr20221635","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T15:02:38Z","timestamp":1680793358000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The effect of epigenetic reprogramming using MI192 HDAC inhibitor on enhancing the osteogenesis of human adipose-derived stem cells <i>in vitro<\/i>"],"prefix":"10.1042","volume":"43","author":[{"given":"Wei","family":"Lu","sequence":"first","affiliation":[{"name":"1Department of Prosthodontics, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China"},{"name":"2Biomaterials and Tissue Engineering Group, School of Dentistry, University of Leeds, Leeds LS9 7TF, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Ji","sequence":"additional","affiliation":[{"name":"3Department of Paediatric Dentistry, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liam","family":"Lawlor","sequence":"additional","affiliation":[{"name":"2Biomaterials and Tissue Engineering Group, School of Dentistry, University of Leeds, Leeds LS9 7TF, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushmita","family":"Saha","sequence":"additional","affiliation":[{"name":"2Biomaterials and Tissue Engineering Group, School of Dentistry, University of Leeds, Leeds LS9 7TF, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiden","family":"Hempshall","sequence":"additional","affiliation":[{"name":"4Molecular Innovation, Diversity and Automated Synthesis (MIDAS) Centre, School of Chemistry, University of Leeds, Leeds LS9 7TF, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Jin","sequence":"additional","affiliation":[{"name":"5State Key Laboratory of Military Stomatology and National Clinical Research, Center for Oral Diseases and Shaanxi Key Laboratory of Oral Diseases, Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0144-2826","authenticated-orcid":false,"given":"Xuebin\u00a0B.","family":"Yang","sequence":"additional","affiliation":[{"name":"2Biomaterials and Tissue Engineering Group, School of Dentistry, University of Leeds, Leeds LS9 7TF, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2023,4,28]]},"reference":[{"key":"2023042810285754200_B1","doi-asserted-by":"publisher","first-page":"e80004","DOI":"10.1371\/journal.pone.0080004","article-title":"Osteochondral tissue engineering in vivo: a comparative study using layered silk fibroin scaffolds from mulberry and nonmulberry silkworms","volume":"8","author":"Saha","year":"2013","journal-title":"PloS ONE"},{"key":"2023042810285754200_B2","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1007\/s00441-013-1586-x","article-title":"Informing future cartilage repair strategies: a comparative study of three different human cell types for cartilage tissue engineering","volume":"352","author":"Saha","year":"2013","journal-title":"Cell Tissue Res."},{"key":"2023042810285754200_B3","first-page":"21","article-title":"Cell-based regenerative therapy as an alternative to liver transplantation for end-stage liver disease: experience from iran","volume":"1","author":"Malekzadeh","year":"2010","journal-title":"Int. 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