{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T07:43:41Z","timestamp":1771919021201,"version":"3.50.1"},"reference-count":18,"publisher":"Portland Press Ltd.","issue":"1","license":[{"start":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:00:00Z","timestamp":1671580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,1,31]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hypoxic-ischemic brain injury contributes to major neurodevelopmental disorders and is one of the leading causes of seizures, which substantially results in neurodevelopmental impairments with long-lasting outcomes and is one of the main causes of death in neonates. We aimed to investigate the correlation between miRNA-210 and SCN1B, a voltage-gated sodium channel gene, in brain tissue of fetal rats with hypoxic-ischemic brain injury. We found that after 10 min of hypoxia-ischemia, all reperfusion groups showed different degrees of damage. The degree of the injury increased in all the groups after 30 min of hypoxia-ischemia. Those changes include changes in the pericellular lumen, capillaries in the cortex, erythrocytes, enlarged pericellular lumen, the enlarged pericapillary lumen in the cortex, edema around glial cells, enlarged gap to form multiple necrotic foci, deformation of neurons, and loss of cell structure. The expression levels of HIF-1\u03b1, miRNA-210, and HIF-1\u03b1 mRNA were higher in the hypoxic-ischemic groups than that in the control groups, among which the expression levels in the severe group were higher than that in mild group. SCN1B is down-regulated in both the mild and severe groups, and the lowest level was found at 30 min after hypoxia in both groups. MiRNA-210 plays a role in the development of hypoxic-ischemic encephalopathy (HIE) by regulating the expression changes of SCN1B. The brain tissue of fetal rats in the hypoxic-ischemic animal model showed pathological changes of brain injury.<\/jats:p>","DOI":"10.1042\/bsr20222016","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T10:19:46Z","timestamp":1671617986000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The relationship between miRNA-210 and SCN1B in fetal rats with hypoxic-ischemic brain injury"],"prefix":"10.1042","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4087-8275","authenticated-orcid":false,"given":"Hisham","family":"Al-Ward","sequence":"first","affiliation":[{"name":"1Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Liu","sequence":"additional","affiliation":[{"name":"2Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2473-5891","authenticated-orcid":false,"given":"Moussa","family":"Omorou","sequence":"additional","affiliation":[{"name":"2Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiwei","family":"Huang","sequence":"additional","affiliation":[{"name":"2Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"1Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"3Department of Rehabilitation therapy, School of Medicine, Ankang University, Ankang 725000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaochun","family":"Lv","sequence":"additional","affiliation":[{"name":"4Provincial Key Laboratory of Microecology Immune Regulation Network and Related Diseases, Basic Medical\u00a0College, Jiamusi University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abduh","family":"Murshed","sequence":"additional","affiliation":[{"name":"5Department of Clinical Laboratory, Shanghai 10th People\u2019s Hospital of Tongji University, Shanghai 200072, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2218-0121","authenticated-orcid":false,"given":"Fahmi","family":"Shaher","sequence":"additional","affiliation":[{"name":"6Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Li","sequence":"additional","affiliation":[{"name":"7Department of Clinical Laboratory Diagnosis, School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"6Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linxia","family":"Lu","sequence":"additional","affiliation":[{"name":"7Department of Clinical Laboratory Diagnosis, School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenxia","family":"Gao","sequence":"additional","affiliation":[{"name":"1Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u00a0Eve","family":"Sun","sequence":"additional","affiliation":[{"name":"1Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Xu","sequence":"additional","affiliation":[{"name":"2Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2023,1,16]]},"reference":[{"key":"2023012511511283300_B1","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1038\/s41372-019-0551-2","article-title":"Characteristics and short-term outcomes of neonates with mild hypoxic-ischemic encephalopathy treated with hypothermia","volume":"40","author":"Goswami","year":"2020","journal-title":"J. Perinatol."},{"key":"2023012511511283300_B2","doi-asserted-by":"publisher","first-page":"22368","DOI":"10.3390\/ijms160922368","article-title":"Neuroprotective strategies after neonatal hypoxic ischemic encephalopathy","volume":"16","author":"Dixon","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"2023012511511283300_B3","doi-asserted-by":"publisher","first-page":"1754","DOI":"10.1055\/s-0041-1726451","article-title":"Preventing brain damage from hypoxic\u2013ischemic encephalopathy in neonates: update on mesenchymal stromal cells and umbilical cord blood cells","volume":"39","author":"Nabetani","year":"2021","journal-title":"Am. J. Perinatol."},{"key":"2023012511511283300_B4","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1159\/000516388","article-title":"Voltage-gated sodium channel \u03b21 gene: an overview","volume":"85","author":"Al-Ward","year":"2020","journal-title":"Hum. Hered."},{"key":"2023012511511283300_B5","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1159\/000509280","article-title":"The role of miR-210 in the biological system: a current overview","volume":"84","author":"Hui","year":"2019","journal-title":"Hum. Hered."},{"key":"2023012511511283300_B6","doi-asserted-by":"publisher","first-page":"4267","DOI":"10.2147\/CMAR.S174476","article-title":"Abnormal microRNA expression in the course of hematological malignancies","volume":"10","author":"Szymczyk","year":"2018","journal-title":"Cancer Manag. Res."},{"key":"2023012511511283300_B7","doi-asserted-by":"publisher","first-page":"1288","DOI":"10.1126\/science.1121566","article-title":"Encountering microRNAs in cell fate signaling","volume":"310","author":"Karp","year":"2005","journal-title":"Science"},{"key":"2023012511511283300_B8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s12253-016-0101-6","article-title":"MicroRNA-mediated post-transcriptional regulation of epithelial to mesenchymal transition in cancer","volume":"23","author":"Behbahani","year":"2017","journal-title":"Pathol. Oncol. Res."},{"key":"2023012511511283300_B9","doi-asserted-by":"publisher","first-page":"1221","DOI":"10.1158\/0008-5472.CAN-08-2516","article-title":"MicroRNA regulation of DNA repair gene expression in hypoxic stress","volume":"69","author":"Crosby","year":"2009","journal-title":"Cancer Res."},{"key":"2023012511511283300_B10","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1002\/0470846682.ch9","article-title":"Beta subunits: players in neuronal hyperexcitability?","volume":"241","author":"Isom","year":"2002","journal-title":"Novartis Found. Symp."},{"key":"2023012511511283300_B11","doi-asserted-by":"publisher","first-page":"628","DOI":"10.1006\/geno.1994.1551","article-title":"Genomic organization and chromosomal assignment of the human voltage-gated Na+ channel \u03b21 subunit gene (SCN1B)","volume":"23","author":"Makita","year":"1994","journal-title":"Genomics"},{"key":"2023012511511283300_B12","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1007\/s00249-004-0389-0","article-title":"Voltage-gated Na+ channels: multiplicity of expression, plasticity, functional implications and pathophysiological aspects","volume":"33","author":"Diss","year":"2004","journal-title":"Eur. Biophys. J."},{"key":"2023012511511283300_B13","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1097\/GIM.0b013e3182217a3a","article-title":"American College of Medical Genetics standards and guidelines for interpretation and reporting of postnatal constitutional copy number variants","volume":"13","author":"Kearney","year":"2011","journal-title":"Genet. Med."},{"key":"2023012511511283300_B14","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1080\/10409238.2020.1738331","article-title":"AGO2 and its partners: a silencing complex, a chromatin modulator, and new features","volume":"55","author":"Li","year":"2020","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"2023012511511283300_B15","doi-asserted-by":"publisher","first-page":"15878","DOI":"10.1074\/jbc.M800731200","article-title":"MicroRNA-210 modulates endothelial cell response to hypoxia and inhibits the receptor tyrosine kinase ligand Ephrin-A3","volume":"283","author":"Fasanaro","year":"2008","journal-title":"J. Biol. Chem."},{"key":"2023012511511283300_B16","doi-asserted-by":"publisher","first-page":"2696","DOI":"10.1128\/MCB.01242-10","article-title":"A hypoxia-induced positive feedback loop promotes hypoxia-inducible factor 1\u03b1 stability through miR-210 suppression of glycerol-3-phosphate dehydrogenase 1-like","volume":"31","author":"Kelly","year":"2011","journal-title":"Mol. Cell. Biol."},{"key":"2023012511511283300_B17","doi-asserted-by":"publisher","first-page":"255","DOI":"10.4161\/cbt.7.2.5297","article-title":"miR-210 links hypoxia with cell cycle regulation and is deleted in human epithelial ovarian cancer","volume":"7","author":"Giannakakis","year":"2008","journal-title":"Cancer Biology & Therapy"},{"key":"2023012511511283300_B18","doi-asserted-by":"publisher","first-page":"2756","DOI":"10.4161\/cc.8.17.9387","article-title":"MicroRNA miR-210 modulates cellular response to hypoxia through the MYC antagonist MNT","volume":"8","author":"Zhang","year":"2009","journal-title":"Cell Cycle"}],"container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20222016\/942157\/bsr-2022-2016.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/doi\/10.1042\/BSR20222016\/942157\/bsr-2022-2016.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,3]],"date-time":"2023-12-03T10:32:27Z","timestamp":1701599547000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/43\/1\/BSR20222016\/232346\/The-relationship-between-miRNA-210-and-SCN1B-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1,31]]}},"URL":"https:\/\/doi.org\/10.1042\/bsr20222016","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,1]]},"published":{"date-parts":[[2023,1]]},"article-number":"BSR20222016"}}