{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T06:40:03Z","timestamp":1780641603160,"version":"3.54.1"},"reference-count":36,"publisher":"Portland Press Ltd.","issue":"11","license":[{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 81101318"],"award-info":[{"award-number":["No. 81101318"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002865","name":"Chongqing Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["No. cstc2021 jx j1130038"],"award-info":[{"award-number":["No. cstc2021 jx j1130038"]}],"id":[{"id":"10.13039\/501100002865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,11,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Objectives: Alanine aminotransferase (ALT) expression is highly elevated in the serum of patients with hepatocellular carcinoma. However, the role of ALT isoenzymes in the total ALT activity remains unclear. In the present study, we systematically investigated the role of ALT isoenzymes in alcoholic and non-alcoholic fatty liver cancer. Materials and methods: The expression of ALT1 and ALT2 at the mRNA and protein levels in 25 paired primary liver cancer tissues was detected by reverse transcription quantitative PCR (RT-qPCR), Western blotting, and immunohistochemistry. Serum ALT activity was determined using an automated biochemical analyzer. Results: The mRNA and protein expression levels of ALT1 and ALT2 were lower in the tissues of alcoholic and non-alcoholic fatty liver cancers than in the paracancerous tissues. Notably, ALT2 was highly expressed in non-alcoholic fatty liver cancer tissues compared with alcoholic fatty liver cancer tissues. Total serum ALT activity was mainly contributed by ALT1 in alcoholic fatty liver cancer, whereas ALT1 contributed only marginally more to the total ALT activity than ALT2 in non-alcoholic fatty liver cancer. ALT2\/ALT1 ratio can well discriminate normal control group, alcoholic liver cancer and non-alcoholic liver cancer. Conclusion: ALT1 contributed more to the total ALT activity than ALT2 in both alcoholic and non-alcoholic fatty liver cancer. Serum ALT2 to ALT activity was higher in non-alcoholic fatty liver cancer than that in alcoholic fatty liver cancer. ALT2\/ALT1 ratio has some diagnostic significance for alcoholic and non-alcoholic liver cancer.<\/jats:p>","DOI":"10.1042\/bsr20230222","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T16:25:28Z","timestamp":1698337528000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Diagnostic significance of alanine aminotransferase isoenzymes in alcoholic and non-alcoholic fatty liver cancers"],"prefix":"10.1042","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1830-4320","authenticated-orcid":false,"given":"Dandan","family":"Zhou","sequence":"first","affiliation":[{"name":"1Department of Clinical Laboratory, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China"},{"name":"2Laboratory for Diagnosis and Treatment of Infectious Diseases Integrated Traditional Chinese and Western Medicine, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuowei","family":"Yuan","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqin","family":"Shu","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hejun","family":"Tang","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangmin","family":"Ye","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana","family":"Tao","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangzhu","family":"Zhou","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"3Artron BioResearch Inc., 3938 North Fraser Way, Burnaby, British Columbia, Canada"},{"name":"4Jinan Kangbo Biotechnology, 2711 Ying Xiu Road, Jinan, Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zheng","sequence":"additional","affiliation":[{"name":"3Artron BioResearch Inc., 3938 North Fraser Way, Burnaby, British Columbia, Canada"},{"name":"4Jinan Kangbo Biotechnology, 2711 Ying Xiu Road, Jinan, Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wu","sequence":"additional","affiliation":[{"name":"5Department of Laboratory Medicine, The Second Affiliated Hospital of Chongqing Medical University, 74 Linjiang Road, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5323-7501","authenticated-orcid":false,"given":"Juan","family":"Zhang","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China"},{"name":"2Laboratory for Diagnosis and Treatment of Infectious Diseases Integrated Traditional Chinese and Western Medicine, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2023,11,3]]},"reference":[{"key":"2023110308444449600_B1","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1002\/dmrr.2655","article-title":"Hepatic ALT isoenzymes are elevated in gluconeogenic conditions including diabetes and suppressed by insulin at the protein level","volume":"31","author":"Qian","year":"2015","journal-title":"Diabetes Metab. 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