{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T14:42:23Z","timestamp":1782139343549,"version":"3.54.5"},"reference-count":43,"publisher":"Portland Press Ltd.","issue":"1","license":[{"start":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T00:00:00Z","timestamp":1701820800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Patanjali Research Foundation Trust","award":["NA"],"award-info":[{"award-number":["NA"]}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,1,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Vitiligo is characterized by the development of white patches on the skin either due to the loss of functional melanocytes or perturbations in the melanogenesis pathway. In the present study, we investigated the therapeutic potential of herbo-mineral formulation, Melanogrit in neutralizing the white patches in the skin. The study utilized UPLC\/MS-QToF technique to determine the diversified phytochemical profile in Melanogrit. The murine B16F10 cells when treated with Melanogrit underwent morphological changes, including increased angularity, enlarged cell size, and greater dendritic protrusions. To establish an equivalent model to study melanogenesis, we carefully optimized the dosage of \u03b1-melanocyte stimulating hormone (\u03b1MSH) in B16F10 cells as an alternative to using melanocyte-keratinocyte cocultures. The study determined a sub-optimal dose of \u03b1MSH (0.2 nM) in B16F10 cells that does not manifest any measurable effects on melanogenesis. In contrast, Melanogrit when used in conjunction with 0.2 nM \u03b1MSH, induced a dose-dependent increase in extracellular and intracellular melanin levels. Melanogrit transcriptionally up-regulated the decisive genes of the melanogenesis pathway, MITF, TYR, and TRP1, which was evident from the increased cellular tyrosine activity. Our findings also demonstrated that Melanogrit ameliorated the MITF protein levels by inhibiting pERK; notably without involving GSK3\u03b2 in the process. Taken together, our findings strongly suggest that Melanogrit has the potential to stimulate melanogenesis, making it a promising candidate for clinical applications in the treatment of white skin patches that develop in vitiligo patients.<\/jats:p>","DOI":"10.1042\/bsr20231324","type":"journal-article","created":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T13:34:18Z","timestamp":1701869658000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Melanogrit potentiates melanogenesis by escalating cellular tyrosinase activity and MITF levels via pERK inhibition"],"prefix":"10.1042","volume":"44","author":[{"given":"Acharya","family":"Balkrishna","sequence":"first","affiliation":[{"name":"1Drug Discovery and Development Division, Patanjali Research Foundation, NH-58, Haridwar 249405, Uttarakhand, India"},{"name":"2Department of Allied and Applied Sciences, University of Patanjali, Patanjali Yog Peeth, Roorkee-Haridwar Road, Haridwar 249405, Uttarakhand, India"},{"name":"3Patanjali Yog Peeth (UK) Trust, 40 Lambhill Street, Kinning Park, Glasgow G41 1AU, U.K."},{"name":"4Vedic Acharya Samaj Foundation, Inc. NFP, 21725 CR 33, Groveland, FL 34736, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savita","family":"Lochab","sequence":"additional","affiliation":[{"name":"1Drug Discovery and Development Division, Patanjali Research Foundation, NH-58, Haridwar 249405, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudeep","family":"Verma","sequence":"additional","affiliation":[{"name":"1Drug Discovery and Development Division, Patanjali Research Foundation, NH-58, Haridwar 249405, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyotish","family":"Srivastava","sequence":"additional","affiliation":[{"name":"1Drug Discovery and Development Division, Patanjali Research Foundation, NH-58, Haridwar 249405, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rishabh","family":"Dev","sequence":"additional","affiliation":[{"name":"1Drug Discovery and Development Division, Patanjali Research Foundation, NH-58, Haridwar 249405, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7878-6934","authenticated-orcid":false,"given":"Anurag","family":"Varshney","sequence":"additional","affiliation":[{"name":"1Drug Discovery and Development Division, Patanjali Research Foundation, NH-58, Haridwar 249405, Uttarakhand, India"},{"name":"2Department of Allied and Applied Sciences, University of Patanjali, Patanjali Yog Peeth, Roorkee-Haridwar Road, Haridwar 249405, Uttarakhand, India"},{"name":"5Special Centre for Systems Medicine, Jawaharlal Nehru University, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2024,1,9]]},"reference":[{"key":"2024010909194173800_B1","doi-asserted-by":"publisher","first-page":"CD003263","DOI":"10.1002\/14651858.CD003263.pub5","article-title":"Interventions for vitiligo","volume":"24","author":"Whitton","year":"2015","journal-title":"Cochrane Database of Systematic Rev."},{"key":"2024010909194173800_B2","doi-asserted-by":"publisher","first-page":"1303","DOI":"10.3390\/molecules22081303","article-title":"Upregulation of melanogenesis and tyrosinase activity: potential agents for vitiligo","volume":"22","author":"Niu","year":"2017","journal-title":"Molecules"},{"key":"2024010909194173800_B3","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1016\/j.jaad.2021.06.880","article-title":"Development and validation of the fingertip unit for assessing Facial Vitiligo Area Scoring Index","volume":"86","author":"Bae","year":"2022","journal-title":"J. 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