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We applied the checkerboard assay using different concentrations of the commercial drug AmB and the synthetic peptides \u03b331-45PvD1++ and \u03b333-41PvD1++ against C. albicans, aiming to find combinations with synergistic interactions. Between these two interactions involving \u03b331-45PvD1++ and AmB, an additive effect was observed. One such interaction occurred at concentrations of 0.009 \u00b5M of peptide \u03b331-45PvD1++ and 13.23 \u00b5M of AmB and another condition of 0.019 \u00b5M of peptide \u03b331-45PvD1++ and 6.61 \u00b5M of AmB. The other two concentrations of the interaction showed a synergistic effect in the combination of synthetic peptide \u03b331-45PvD1++ and AmB, where the concentrations were 1.40 \u00b5M peptide \u03b331-45PvD1++ and 0.004 \u00b5M AmB and 0.70 \u00b5M \u03b331-45PvD1++ peptide and 0.002 \u00b5M AmB. We proceeded with analysis of the mechanism of action involving synergistic effects. This examination unveiled a range of impactful outcomes, including the impairment of mitochondrial functionality, compromise of cell wall integrity, DNA degradation, and a consequential decline in cell viability. We also observed that both synergistic combinations were capable of causing damage to the plasma membrane and cell wall, causing leakage of intracellular components. This discovery demonstrates for the first time that the synergistic combinations found between the synthetic peptide \u03b331-45PvD1++ and AmB have an antifungal effect against C. albicans, acting on the integrity of the plasma membrane and cell wall.<\/jats:p>","DOI":"10.1042\/bsr20232075","type":"journal-article","created":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T08:29:08Z","timestamp":1712046548000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Synergistic action of synthetic peptides and amphotericin B causes disruption of the plasma membrane and cell wall in <i>Candida albicans<\/i>"],"prefix":"10.1042","volume":"44","author":[{"given":"Thayna\u00a0A.M.","family":"Souza","sequence":"first","affiliation":[{"name":"1Laborat\u00f3rio de Fisiologia e Bioqu\u00edmica de Microrganismos, Centro de Bioci\u00eancias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, CEP: 28013-602, Campos dos Goytacazes, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica\u00a0O.","family":"Mello","sequence":"additional","affiliation":[{"name":"1Laborat\u00f3rio de Fisiologia e Bioqu\u00edmica de Microrganismos, Centro de Bioci\u00eancias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, CEP: 28013-602, Campos dos Goytacazes, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel\u00a0B.","family":"Taveira","sequence":"additional","affiliation":[{"name":"1Laborat\u00f3rio de Fisiologia e Bioqu\u00edmica de Microrganismos, Centro de Bioci\u00eancias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, CEP: 28013-602, Campos dos Goytacazes, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe\u00a0F.","family":"Moreira","sequence":"additional","affiliation":[{"name":"2Laborat\u00f3rio de Biologia Celular e Tecidual, Centro de Bioci\u00eancias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio\u00a0Henrique","family":"Seabra","sequence":"additional","affiliation":[{"name":"2Laborat\u00f3rio de Biologia Celular e Tecidual, Centro de Bioci\u00eancias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9\u00a0O.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"1Laborat\u00f3rio de Fisiologia e Bioqu\u00edmica de Microrganismos, Centro de Bioci\u00eancias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, CEP: 28013-602, Campos dos Goytacazes, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4145-428X","authenticated-orcid":false,"given":"Valdirene\u00a0M.","family":"Gomes","sequence":"additional","affiliation":[{"name":"1Laborat\u00f3rio de Fisiologia e Bioqu\u00edmica de Microrganismos, Centro de Bioci\u00eancias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, CEP: 28013-602, Campos dos Goytacazes, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2024,4,12]]},"reference":[{"key":"2024041207453290000_B1","doi-asserted-by":"publisher","first-page":"4099","DOI":"10.1016\/j.vaccine.2021.06.001","article-title":"Synthetic conjugate peptide Fba-Met6 (MP12) induces complement-mediated resistance against disseminated Candida albicans","volume":"39","author":"Adams","year":"2021","journal-title":"Vaccine"},{"key":"2024041207453290000_B2","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1128\/AAC.01921-19","article-title":"Identification of off-patent drugs that show synergism with amphotericin B or that present antifungal action against Cryptococcus neoformans and Candida spp","volume":"64","author":"Rossi","year":"2020","journal-title":"Antimicrob. 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