{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:38:26Z","timestamp":1753871906068,"version":"3.41.2"},"reference-count":37,"publisher":"Portland Press Ltd.","issue":"7","license":[{"start":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T00:00:00Z","timestamp":1718150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Direkt\u00f6r Albert P\u00e5hlssons Foundation"},{"name":"Royal Physiographic Society of Lund"},{"name":"FANG Foundation"},{"name":"Ulla Hamberg Angeby och Lennart Angebys Stiftelse"},{"name":"Foundation for Geriatric Diseases at Karolinska Institutet"},{"DOI":"10.13039\/501100003792","name":"Hj\u00e4rnfonden","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003792","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100023867","name":"Swedish Stroke Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100023867","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Obesity and Type 2 diabetes (T2D) are known to exacerbate cerebral injury caused by stroke. Metabolomics can provide signatures of metabolic disease, and now we explored whether the analysis of plasma metabolites carries biomarkers of how obesity and T2D impact post-stroke recovery. Male mice were fed a high-fat diet (HFD) for 10 months leading to development of obesity with T2D or a standard diet (non-diabetic mice). Then, mice were subjected to either transient middle cerebral artery occlusion (tMCAO) or sham surgery and allowed to recover on standard diet for 2 months before serum samples were collected. Nuclear magnetic resonance (NMR) spectroscopy of serum samples was used to investigate metabolite signals and metabolic pathways that were associated with tMCAO recovery in either T2D or non-diabetic mice. Overall, after post-stroke recovery there were different serum metabolite profiles in T2D and non-diabetic mice. In non-diabetic mice, which show full neurological recovery after stroke, we observed a reduction of isovalerate, and an increase of kynurenate, uridine monophosphate, gluconate and N6-acetyllysine in tMCAO relative to sham mice. In contrast, in mice with T2D, which show impaired stroke recovery, there was a reduction of N,N-dimethylglycine, succinate and proline, and an increase of 2-oxocaproate in serum of tMCAO versus sham mice. Given the inability of T2D mice to recover from stroke, in contrast with non-diabetic mice, we propose that these specific metabolite changes following tMCAO might be used as biomarkers of neurophysiological recovery after stroke in T2D.<\/jats:p>","DOI":"10.1042\/bsr20240249","type":"journal-article","created":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T11:59:34Z","timestamp":1718193574000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Nuclear magnetic resonance spectroscopy reveals biomarkers of stroke recovery in a mouse model of obesity-associated type 2 diabetes"],"prefix":"10.1042","volume":"44","author":[{"given":"Jo\u00e3o\u00a0P.P.","family":"Vieira","sequence":"first","affiliation":[{"name":"1Diabetes and Brain Function Unit, Department of Experimental Medical Science, Faculty of Medicine, Lund University, 221 84 Lund, Sweden"},{"name":"2Wallenberg Centre for Molecular Medicine, Lund University, 221 84 Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitra","family":"Karampatsi","sequence":"additional","affiliation":[{"name":"3NeuroCardioMetabol Group, Department of Clinical Science and Education, S\u00f6dersjukhuset, Internal Medicine, Karolinska Institutet, 118 83 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen","family":"Vercalsteren","sequence":"additional","affiliation":[{"name":"3NeuroCardioMetabol Group, Department of Clinical Science and Education, S\u00f6dersjukhuset, Internal Medicine, Karolinska Institutet, 118 83 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimer","family":"Darsalia","sequence":"additional","affiliation":[{"name":"3NeuroCardioMetabol Group, Department of Clinical Science and Education, S\u00f6dersjukhuset, Internal Medicine, Karolinska Institutet, 118 83 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0470-4606","authenticated-orcid":false,"given":"Cesare","family":"Patrone","sequence":"additional","affiliation":[{"name":"3NeuroCardioMetabol Group, Department of Clinical Science and Education, S\u00f6dersjukhuset, Internal Medicine, Karolinska Institutet, 118 83 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5984-1574","authenticated-orcid":false,"given":"Joao\u00a0M.N.","family":"Duarte","sequence":"additional","affiliation":[{"name":"1Diabetes and Brain Function Unit, Department of Experimental Medical Science, Faculty of Medicine, Lund University, 221 84 Lund, Sweden"},{"name":"2Wallenberg Centre for Molecular Medicine, Lund University, 221 84 Lund, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2024,7,5]]},"reference":[{"key":"2024070509205441000_B1","doi-asserted-by":"publisher","first-page":"1269","DOI":"10.1016\/S0140-6736(18)31269-8","article-title":"Prevention of stroke: a global perspective","volume":"392","author":"Pandian","year":"2018","journal-title":"Lancet"},{"key":"2024070509205441000_B2","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1038\/s41574-019-0176-8","article-title":"Obesity: global epidemiology and pathogenesis","volume":"15","author":"Bl\u00fcher","year":"2019","journal-title":"Nat. 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