{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:19:12Z","timestamp":1772137152726,"version":"3.50.1"},"reference-count":41,"publisher":"Portland Press Ltd.","issue":"9","license":[{"start":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T00:00:00Z","timestamp":1724976000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005297","name":"Uniwersytet Medyczny w Bialymstoku","doi-asserted-by":"crossref","award":["SUB\/2\/DN\/20\/001\/2201"],"award-info":[{"award-number":["SUB\/2\/DN\/20\/001\/2201"]}],"id":[{"id":"10.13039\/501100005297","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,9,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Purpose: The aim of the present study was to establish the role of platelets and activated factor XIIIa (FXIIIa) in the structuring of the fibrin network as well as to clarify the effect of network compaction on clot lysis.<\/jats:p>\n                  <jats:p>Methods: Turbidimetry was used for the one-stage clotting test where platelet-free plasma (PFP) is regarded as single factor-deficient plasma (platelets as lacking factor) and autologous platelet-rich plasma (PRP) as deficiency corrected plasma. Structural features of the developed and subsequently lysed fibrin network, formed under static and flow conditions, were visualized by confocal microscopy. Results: Thrombin-initiated plasma clotting revealed changes in the shape of the absorption curve, more pronounced in the presence of platelets. These changes correlate with the transformation of the fibrin scaffold during clot maturing. With the combined action of platelets, thrombin and Ca2+, plasma clotting passes through two phases: initial formation of a platelet-fibrin network (first peak in the polymerization curve), and then the compaction of fibrin, driven by FXIIIa (the second peak) which can be further modulate by the contractile action of platelets. These structural changes, mediated by platelets and FXIIIa, have been shown to determine subsequent clot lysis.<\/jats:p>\n                  <jats:p>Conclusions: Platelet aggregates serve as organizing centers that determine the distribution of fibrin in clot volume. The openwork structure of the platelet-transformed fibrin provides the necessary prerequisites for its timely lysis. The revealed aspects of the interaction of platelets and FXIIIa, which accompanies the maturation of a fibrin clot, may lead to new approaches in the pharmacological correction of disorders associated with both thrombotic episodes and bleeding tendency.<\/jats:p>","DOI":"10.1042\/bsr20240332","type":"journal-article","created":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T09:30:40Z","timestamp":1725010240000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Integration of clotting and fibrinolysis: central role of platelets and factor XIIIa"],"prefix":"10.1042","volume":"44","author":[{"given":"Irina","family":"Patalakh","sequence":"first","affiliation":[{"name":"1Department of Chemistry and Biochemistry of Enzymes, Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Revka","sequence":"additional","affiliation":[{"name":"1Department of Chemistry and Biochemistry of Enzymes, Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agata","family":"Go\u0142aszewska","sequence":"additional","affiliation":[{"name":"2Department of General and Experimental Pathology, Medical University of Bia\u0142ystok, 15089 Bia\u0142ystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Bielicka","sequence":"additional","affiliation":[{"name":"3Department of Biopharmacy and Radiopharmacy, Medical University of Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5403-9467","authenticated-orcid":false,"given":"Tomasz","family":"Misztal","sequence":"additional","affiliation":[{"name":"4Department of Physical Chemistry, Medical University of Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2024,9,23]]},"reference":[{"key":"2024092305465430200_B1","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.blre.2006.11.001","article-title":"Thrombin generation and fibrin clot structure","volume":"21","author":"Wolberg","year":"2007","journal-title":"Blood Rev."},{"key":"2024092305465430200_B2","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1111\/jth.12045","article-title":"Platelet-based coagulation: different populations, different functions","volume":"11","author":"Heemskerk","year":"2013","journal-title":"J. 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