{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:08:03Z","timestamp":1785409683355,"version":"3.56.0"},"reference-count":39,"publisher":"Portland Press Ltd.","issue":"8","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/n\/a","name":"Program for New Clinical Techniques and Therapies of Peking University School and Hospital of Stomatology","doi-asserted-by":"publisher","award":["PKUSSNCT-18A01"],"award-info":[{"award-number":["PKUSSNCT-18A01"]}],"id":[{"id":"10.13039\/n\/a","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/n\/a","name":"Program for New Clinical Techniques and Therapies of Peking University School and Hospital of Stomatology","doi-asserted-by":"publisher","award":["PKUSSNCT-19A04"],"award-info":[{"award-number":["PKUSSNCT-19A04"]}],"id":[{"id":"10.13039\/n\/a","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/n\/a","name":"National Program for Multidisciplinary Cooperative Treatment on Major Diseases","doi-asserted-by":"publisher","award":["PKUSSNMP-202019"],"award-info":[{"award-number":["PKUSSNMP-202019"]}],"id":[{"id":"10.13039\/n\/a","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,8,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Pemphigus vulgaris (PV) is a life-threatening autoimmune bullous disease with an unclear pathogenesis. Our previous study indicated that the expression of miR-125b-5p was significantly elevated in PV patients. MiR-125b-5p has been reported to target P63 mRNA and inhibit the expression of P63, while p53-apoptosis-effector-related-to-PMP-22 (PERP) is a downstream protein regulated by P63. However, the role and underlying molecular mechanisms of miR-125b-5p in PV remain unclear. Here, we explored the roles of miR-125b-5p, P63, and PERP in regulating desmosome integrity and their contribution to PV pathogenesis. The dual-luciferase reporter assay showed that miR-125b-5p inhibited P63 expression by directly binding to the 3\u2032 UTR of P63 mRNA. Mouse tongue tissues were cultured in vitro and treated with desmoglein (Dsg) 3-monoclonal antibody (mAb) or with miR-125b-5p overexpression before Dsg3-mAb treatment. Overexpression of miR-125b-5p significantly reduced the mRNA and protein levels of P63 and PERP in Dsg3-mAb-treated tongue tissue. Transmission electron microscopy analysis of the structure of desmosomes revealed that, in tongue tissue with miR-125b-5p overexpression and Dsg3-mAb treatment, the interdesmosal width was significantly increased, the thickness of keratin insertion into the membrane was markedly reduced, and the desmosome structures exhibited evident damage. Together, these findings indicate that miR-125b-5p reduced the expression of both P63 and PERP by targeting P63, thereby contributing to the disruption of desmosome integrity.<\/jats:p>","DOI":"10.1042\/bsr20260220","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T08:27:56Z","timestamp":1783931276000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["MiR-125b-5p promotes pemphigus vulgaris pathogenesis by modulating desmosome structures"],"prefix":"10.1042","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1635-7437","authenticated-orcid":false,"given":"Wenxiu","family":"He","sequence":"first","affiliation":[{"name":"1Department of Stomatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China"},{"name":"2Department of Oral Medicine, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, PR China"},{"name":"3Division of Oral & Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingning","family":"Xiao","sequence":"additional","affiliation":[{"name":"2Department of Oral Medicine, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1198-508X","authenticated-orcid":false,"given":"Hong","family":"Hua","sequence":"additional","affiliation":[{"name":"2Department of Oral Medicine, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixiao","family":"Xing","sequence":"additional","affiliation":[{"name":"2Department of Oral Medicine, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, PR China"},{"name":"4Department of Stomatology, Zhongshan Hospital, Fudan University, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panpan","family":"Shang","sequence":"additional","affiliation":[{"name":"5Department of Dermatology, Peking University First Hospital & National Clinical Research Center for Skin and Immune Diseases & Beijing Key Laboratory of Molecular Diagnosis on Dermatoses & National Medical Products Administration Key Laboratory for Quality Control and Evaluation of Cosmetics, Beijing, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingya","family":"Fu","sequence":"additional","affiliation":[{"name":"6First Clinical Division, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7593-0208","authenticated-orcid":false,"given":"Li\u00a0Wu","family":"Zheng","sequence":"additional","affiliation":[{"name":"3Division of Oral & Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8938-6880","authenticated-orcid":false,"given":"Pan","family":"Wei","sequence":"additional","affiliation":[{"name":"2Department of Oral Medicine, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"2026073006365232000_B1","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1111\/1346-8138.12486","article-title":"Japanese guidelines for the management of 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