{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:44:52Z","timestamp":1791877492879,"version":"4.3.4"},"reference-count":43,"publisher":"Portland Press Ltd.","issue":"10","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017642","name":"MEC | Spanish National Plan for Scientific and Technical Research and Innovation","doi-asserted-by":"publisher","award":["PID2019-103868RB-I00"],"award-info":[{"award-number":["PID2019-103868RB-I00"]}],"id":[{"id":"10.13039\/501100017642","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003030","name":"Government of Catalonia | Ag\u00e8ncia de Gesti\u00f3 d'Ajuts Universitaris i de Recerca","doi-asserted-by":"publisher","award":["2021 SGR 00068"],"award-info":[{"award-number":["2021 SGR 00068"]}],"id":[{"id":"10.13039\/501100003030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,10,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The intracellular unorthodox aquaporins, AQP11 and AQP12, remain the least characterized members of the aquaporin superfamily. Mammalian and teleost AQP12 channels have recently been established as polytransporters in native membranes. They are localized intracellularly in pancreatic acinar cells and considered to play roles in zymogen granule maturation and exocytosis. In contrast with the vertebrate channels, the permeability properties and physiological roles of invertebrate AQP12-like (Aqp12L) orthologs are completely unknown. Here, we report that the Aqp12L channel from the extremophile crustacean Artemia franciscana is specifically targeted to the membrane of the yolk platelets (YPs) when heterologously expressed in frog (Xenopus laevis) oocytes, as recently reported for the vertebrate orthologs. Using YPs isolated from A. franciscana Aqp12L-expressing frog oocytes, we further show that this channel can transport water, H2O2, ammonia, and urea but not glycerol. Immunostaining of the A. franciscana ovary using a specific Aqp12L antibody revealed that the in vivo localization of the channel also occurs in the YP membranes of vitellogenic oocytes. RNAi-mediated silencing of Aqp12L substantially reduces the offspring output of both ovoviviparous and oviparous females through the impairment of oocyte vitellogenesis (oocyte growth) and the premature activation of ovarian apoptotic and atretic pathways. These findings provide the first direct evidence demonstrating that invertebrate Aqp12L is a functional polytransporter with an essential role in crustacean oogenesis.<\/jats:p>","DOI":"10.1042\/bsr20260496","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:22:13Z","timestamp":1790781733000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The unorthodox aquaporin-12L ortholog is essential for oogenesis in the crustacean\n                    <i>Artemia franciscana<\/i>"],"prefix":"10.1042","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0060-013X","authenticated-orcid":false,"given":"\u00c1ngel","family":"Rey-Alfonso","sequence":"first","affiliation":[{"name":"1Institute of Marine Sciences, Spanish National Research Council (CSIC), 08003 Barcelona, Spain"},{"name":"2Institute of Biotechnology and Biomedicine (IBB), Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra (Cerdanyola del Vall\u00e8s), Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5571-7517","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Chauvign\u00e9","sequence":"additional","affiliation":[{"name":"1Institute of Marine Sciences, Spanish National Research Council (CSIC), 08003 Barcelona, Spain"},{"name":"2Institute of Biotechnology and Biomedicine (IBB), Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra (Cerdanyola del Vall\u00e8s), Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8776-3945","authenticated-orcid":false,"given":"Roderick\u00a0Nigel","family":"Finn","sequence":"additional","affiliation":[{"name":"1Institute of Marine Sciences, Spanish National Research Council (CSIC), 08003 Barcelona, Spain"},{"name":"2Institute of Biotechnology and Biomedicine (IBB), Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra (Cerdanyola del Vall\u00e8s), Spain"},{"name":"3Department of Biological Sciences, University of Bergen, 5006 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2568-6398","authenticated-orcid":false,"given":"Joan","family":"Cerd\u00e0","sequence":"additional","affiliation":[{"name":"1Institute of Marine Sciences, Spanish National Research Council (CSIC), 08003 Barcelona, Spain"},{"name":"2Institute of Biotechnology and Biomedicine (IBB), Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra (Cerdanyola del Vall\u00e8s), Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2026,10,13]]},"reference":[{"key":"2026101302470447800_B1","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1038\/nrm1469","article-title":"From structure to disease: the evolving tale of aquaporin biology","volume":"5","author":"King","year":"2004","journal-title":"Nat. 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